Related ArticlesADAPT-NMR 3.0: utilization of BEST-type triple-resonance NMR experiments to accelerate the process of data collection and assignment.
J Biomol NMR. 2015 Jul;62(3):247-52
Authors: Dashti H, Tonelli M, Markley JL
Abstract
ADAPT-NMR (Assignment-directed Data collection Algorithm utilizing a Probabilistic Toolkit in NMR) is a software package whose Bayesian core uses on-the-fly chemical shift assignments to guide data acquisition by non-uniform sampling from a panel of through-bond NMR experiments. The new version of ADAPT-NMR (ADAPT-NMR v3.0) has the option of utilizing 2D tilted-plane versions of 3D fast spectral acquisition with BEST-type pulse sequences, while also retaining the capability of acquiring and processing data from tilted-plane versions of conventional sensitivity-enhanced experiments. The use of BEST experiments significantly reduces data collection times and leads to enhanced performance by ADAPT-NMR.
ADAPT-NMR 3.0: utilization of BEST-type triple-resonance NMR experiments to accelerate the process of data collection and assignment
ADAPT-NMR 3.0: utilization of BEST-type triple-resonance NMR experiments to accelerate the process of data collection and assignment
Abstract
ADAPT-NMR (Assignment-directed Data collection Algorithm utilizing a Probabilistic Toolkit in NMR) is a software package whose Bayesian core uses on-the-fly chemical shift assignments to guide data acquisition by non-uniform sampling from a panel of through-bond NMR experiments. The new version of ADAPT-NMR (ADAPT-NMR v3.0) has the option of utilizing 2D tilted-plane versions of 3D fast spectral acquisition with...
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05-29-2015 01:24 PM
Triple resonance-based 13 C α and 13 C β CEST experiments for studies of ms timescale dynamics in proteins
Triple resonance-based 13 C α and 13 C β CEST experiments for studies of ms timescale dynamics in proteins
Abstract
A pair of triple resonance based CEST pulse schemes are presented for measuring 13Cα and 13Cβ chemical shifts of sparsely populated and transiently formed conformers that are invisible to traditional NMR experiments. CEST profiles containing dips at resonance positions of 13Cα or 13Cβ spins of major (ground) and minor (excited) conformers are obtained in a pseudo 3rd dimension that is generated by quantifying modulations of cross...
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10-28-2014 02:42 PM
Time-shared experiments for efficient assignment of triple-selectively labeled proteins
Time-shared experiments for efficient assignment of triple-selectively labeled proteins
Publication date: Available online 30 September 2014
Source:Journal of Magnetic Resonance</br>
Author(s): Frank Löhr , Aisha Laguerre , Christoph Bock , Sina Reckel , Peter J. Connolly , Norzehan Abdul-Manan , Franz Tumulka , Rupert Abele , Jonathan M. Moore , Volker Dötsch</br>
Combinatorial triple-selective labeling facilitates the NMR assignment process for proteins that are subject to signal overlap and insufficient signal-to-noise in standard triple-resonance...
[NMR paper] Fast automated protein NMR data collection and assignment by ADAPT-NMR on Bruker spectrometers.
Fast automated protein NMR data collection and assignment by ADAPT-NMR on Bruker spectrometers.
Related Articles Fast automated protein NMR data collection and assignment by ADAPT-NMR on Bruker spectrometers.
J Magn Reson. 2013 Aug 30;236C:83-88
Authors: Lee W, Hu K, Tonelli M, Bahrami A, Neuhardt E, Glass KC, Markley JL
Abstract
ADAPT-NMR (Assignment-directed Data collection Algorithm utilizing a Probabilistic Toolkit in NMR) supports automated NMR data collection and backbone and side chain assignment for -labeled proteins. Given the...
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10-06-2013 06:11 AM
[NMR paper] Fast automated protein NMR data collection and assignment by ADAPT-NMR on Bruker spectrometers
Fast automated protein NMR data collection and assignment by ADAPT-NMR on Bruker spectrometers
Publication date: Available online 30 August 2013
Source:Journal of Magnetic Resonance</br>
Author(s): Woonghee Lee , Kaifeng Hu , Marco Tonelli , Arash Bahrami , Elizabeth Neuhardt , Karen C. Glass , John L. Markley</br>
ADAPT-NMR (Assignment-directed Data collection Algorithm utilizing a Probabilistic Toolkit in NMR) supports automated NMR data collection and backbone and side chain assignment for -labeled proteins. Given the sequence of the protein and data for...
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08-30-2013 04:35 PM
Pseudo-4D triple resonance experiments to resolve HN overlap in the backbone assignment of unfolded proteins
Pseudo-4D triple resonance experiments to resolve HN overlap in the backbone assignment of unfolded proteins
Abstract The solution NMR resonance assignment of the protein backbone is most commonly carried out using triple resonance experiments that involve 15N and 1HN resonances. The assignment becomes problematic when there is resonance overlap of 15Nâ??1HN cross peaks. For such residues, one cannot unambiguously link the â??leftâ?? side of the NH root to the â??rightâ?? side, and the residues associated with such overlapping HN resonances remain often unassigned. Here we present a...
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12-31-2010 08:38 PM
[NMR paper] Double and triple resonance NMR methods for protein assignment.
Double and triple resonance NMR methods for protein assignment.
Related Articles Double and triple resonance NMR methods for protein assignment.
Methods Mol Biol. 1997;60:29-52
Authors: Whitehead B, Craven CJ, Waltho JP
ADAPT-NMR 3.0: utilization of BEST-type triple-resonance NMR experiments to accelerate the process of data collection and assignment.
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