[NMR paper] Activity-Based Probes Developed by Applying Sequential Dehydroalanine Strategy on Expressed Proteins Reveal a Potential ?-globin Modulating Deubiquitinase
Activity-Based Probes Developed by Applying Sequential Dehydroalanine Strategy on Expressed Proteins Reveal a Potential ?-globin Modulating Deubiquitinase
We report a general and novel semisynthetic strategy for the preparation of ubiquitinated protein activity based probes applying sequential dehydroalanine formation on expressed proteins. We applied this approach to construct physiologically and therapeutically relevant ubiquitinated ?-globin probe, which was used for the enrichment and proteomic identification of ?-globin modulating deubiquitinases. We found USP15 as a potential deubiquitinase, modulating ?-globin, which excess aggravates beta-thalassemia symptoms. This development opens new opportunities for the activity based probe design to shed light on the important aspects underlining ubiquitination and deubiquitination in health and disease.
[NMR paper] Salecan protected against concanavalin A-induced acute liver injury by modulating T cell immune responses and NMR-based metabolic profiles.
Salecan protected against concanavalin A-induced acute liver injury by modulating T cell immune responses and NMR-based metabolic profiles.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Salecan protected against concanavalin A-induced acute liver injury by modulating T cell immune responses and NMR-based metabolic profiles.
Toxicol Appl Pharmacol. 2017 Feb 15;317:63-72
Authors: Sun Q, Xu X, Yang X, Weng D, Wang J, Zhang J
Abstract
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[NMR paper] Defining the Potential of Aglycone Modifications for Affinity/Selectivity Enhancement against Medically Relevant Lectins: Synthesis, Activity Screening, and HSQC-Based NMR Analysis.
Defining the Potential of Aglycone Modifications for Affinity/Selectivity Enhancement against Medically Relevant Lectins: Synthesis, Activity Screening, and HSQC-Based NMR Analysis.
Related Articles Defining the Potential of Aglycone Modifications for Affinity/Selectivity Enhancement against Medically Relevant Lectins: Synthesis, Activity Screening, and HSQC-Based NMR Analysis.
Chembiochem. 2014 Nov 18;
Authors: Rauthu SR, Shiao TC, André S, Miller MC, Madej E, Mayo KH, Gabius HJ, Roy R
Abstract
The emerging significance of...
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[NMR paper] Solution NMR Structures of Active Pyrenophora tritici-repentis ToxB and its Inactive Homolog Reveal Potential Determinants of Toxin Activity.
Solution NMR Structures of Active Pyrenophora tritici-repentis ToxB and its Inactive Homolog Reveal Potential Determinants of Toxin Activity.
Related Articles Solution NMR Structures of Active Pyrenophora tritici-repentis ToxB and its Inactive Homolog Reveal Potential Determinants of Toxin Activity.
J Biol Chem. 2014 Jul 26;
Authors: Nyarko A, Singarapu KK, Figueroa M, Manning VA, Pandelova I, Wolpert TJ, Ciuffetti LM, Barbar E
Abstract
Ptr ToxB (ToxB) is a proteinaceous host-selective toxin produced by Pyrenophora...
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07-30-2014 10:22 AM
[NMR paper] An approach to sequential NMR assignments of proteins: application to chemical shift restraint-based structure prediction.
An approach to sequential NMR assignments of proteins: application to chemical shift restraint-based structure prediction.
Related Articles An approach to sequential NMR assignments of proteins: application to chemical shift restraint-based structure prediction.
J Biomol NMR. 2014 Jun 19;
Authors: Wiedemann C, Bellstedt P, Herbst C, Görlach M, Ramachandran R
Abstract
A procedure for the simultaneous acquisition of {HNCOCANH & HCCCONH} chemical shift correlation spectra employing sequential data acquisition for moderately sized...
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An approach to sequential NMR assignments of proteins: application to chemical shift restraint-based structure prediction
An approach to sequential NMR assignments of proteins: application to chemical shift restraint-based structure prediction
Abstract
A procedure for the simultaneous acquisition of {HNCOCANH & HCCCONH} chemical shift correlation spectra employing sequential \(^{1}\hbox {H}\) data acquisition for moderately sized proteins is presented. The suitability of the approach for obtaining sequential resonance assignments, including complete ...
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06-19-2014 10:21 PM
Synthesis and Characterization of [S2MoS2Cu(n-SPhF)]2- (n=o, m, p) Clusters: Potential 19F-NMR Structural Probes for Orange Protein
Synthesis and Characterization of 2- (n=o, m, p) Clusters: Potential 19F-NMR Structural Probes for Orange Protein
Publication date: Available online 18 April 2014
Source:Inorganic Chemistry Communications</br>
Author(s): Biplab K. Maiti , Teresa Avilés , Isabel Moura , Sofia R. Pauleta , José J.G. Moura</br>
Three fluorinated Mo-Cu-thiolate isomers, 2, , 3-fluorothiophenol (1b), and 4-fluorothiophenol (1c)] were synthesized and spectroscopically characterized. The 19 F-NMR signal of the fluorine atom in the benzene has different chemical shift for each isomer,...
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[NMR paper] A new strategy for sequential assignment of intrinsically unstructured proteins based on 15N single isotope labelling
A new strategy for sequential assignment of intrinsically unstructured proteins based on 15N single isotope labelling
Publication date: Available online 23 July 2013
Source:Journal of Magnetic Resonance</br>
Author(s): Juan Lopez , Puneet Ahuja , Melanie Gérard , Jean Michel Wieruszeski , Guy Lippens</br>
We describe a new efficient strategy for the sequential assignment of amide resonances of a conventional 15N-1H HSQC spectrum of intrinsically unfolded proteins, based on composite NOESY-TOCSY and TOCSY-NOESY mixing times. These composite mixing times lead to a...
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[NMR paper] Advantage of Applying OSC to (1)H NMR-Based Metabonomic Data of Celiac Disease.
Advantage of Applying OSC to (1)H NMR-Based Metabonomic Data of Celiac Disease.
Advantage of Applying OSC to (1)H NMR-Based Metabonomic Data of Celiac Disease.
Int J Endocrinol Metab. 2012;10(3):548-552
Authors: Rezaei-Tavirani M, Fathi F, Darvizeh F, Zali MR, Rostami Nejad M, Rostami K, Tafazzoli M, Arefi Oskouie A, Mortazavi-Tabatabaei SA
Abstract
BACKGROUND: Celiac disease (CD) is a disorder associated with body reaction to gluten. After the gluten intake, an immune reaction against the protein occurs and damages villi of small...
Activity-Based Probes Developed by Applying Sequential Dehydroalanine Strategy on Expressed Proteins Reveal a Potential ?-globin Modulating Deubiquitinase
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