[NMR paper] Acetonitrile allows indirect replacement of non-deuterated lipid detergents by deuterated lipid detergents for the NMR study of detergent-soluble proteins
Acetonitrile allows indirect replacement of non-deuterated lipid detergents by deuterated lipid detergents for the NMR study of detergent-soluble proteins
Detergent-soluble proteins (DSPs) are commonly dissolved in lipid buffers for NMR experiments, but the huge lipid proton signal prevents recording of high-quality spectra. The use of costly deuterated lipids is thus required to replace non-deuterated ones. With conventional methods, detergents like dodecylphosphocholine (DPC) cannot be fully exchanged due to their high binding affinity to hydrophobic proteins. We propose an original and simple protocol which combines the use of acetonitrile,...
Acetonitrile allows indirect replacement of non-deuterated lipid detergents by deuterated lipid detergents for the NMR study of detergent-soluble proteins
Acetonitrile allows indirect replacement of non-deuterated lipid detergents by deuterated lipid detergents for the NMR study of detergent-soluble proteins
Abstract
Detergent-soluble proteins (DSPs) are commonly dissolved in lipid buffers for NMR experiments, but the huge lipid proton signal prevents recording of high-quality spectra. The use of costly deuterated lipids is thus required to replace non-deuterated ones. With conventional methods, detergents like dodecylphosphocholine (DPC) cannot be fully exchanged due to their high binding affinity to hydrophobic proteins. We propose an...
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08-31-2021 10:53 AM
[ASAP] Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents
Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.0c00924/20210323/images/medium/bi0c00924_0008.gif
Biochemistry
DOI: 10.1021/acs.biochem.0c00924
http://feeds.feedburner.com/~r/acs/bichaw/~4/1vlEUpFW6vM
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[NMR paper] Detergent titration as an efficient method for NMR resonance assignments of membrane proteins in lipid-bilayer nanodiscs.
Detergent titration as an efficient method for NMR resonance assignments of membrane proteins in lipid-bilayer nanodiscs.
Related Articles Detergent titration as an efficient method for NMR resonance assignments of membrane proteins in lipid-bilayer nanodiscs.
Anal Chem. 2020 May 07;:
Authors: Bibow S, Böhm R, Modaresi SM, Hiller S
Abstract
Lipid bilayer nanodiscs are an attractive tool to study membrane proteins in a detergent-free lipid-bilayer environment. In the case of NMR studies, a sequence-specific resonance assignment is...
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05-08-2020 06:11 PM
[NMR paper] Reconstitution and NMR Characterization of the Ion-Channel Accessory Subunit Barttin in Detergents and Lipid-Bilayer Nanodiscs.
Reconstitution and NMR Characterization of the Ion-Channel Accessory Subunit Barttin in Detergents and Lipid-Bilayer Nanodiscs.
Related Articles Reconstitution and NMR Characterization of the Ion-Channel Accessory Subunit Barttin in Detergents and Lipid-Bilayer Nanodiscs.
Front Mol Biosci. 2019;6:13
Authors: Viennet T, Bungert-Plümke S, Elter S, Viegas A, Fahlke C, Etzkorn M
Abstract
Barttin is an accessory subunit of ClC-K chloride channels expressed in the kidney and the inner ear. Main functions of ClC-K/barttin channels are...
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04-02-2019 02:58 PM
[NMR paper] Dicarboxylate-Oside Detergents for Extraction, Overstabilization and Crystallization of Membrane Proteins
Dicarboxylate-Oside Detergents for Extraction, Overstabilization and Crystallization of Membrane Proteins
To tackle the problem of membrane proteins (MPs) instability in detergent solutions, we designed a series of dicarboxylate-oside detergents (DCODs) in which we optimized the polar head to clamp the membrane domain, including on one side a cluster of two carboxyl groups that promotes salt-bridges with basic residues abundant at the membrane-cytoplasm interface of MPs, and on the other side a sugar to generate H-bonds. Tested on BmrA, an ATP-binding cassette pump, DCODs 8b, 8c, and 9b...
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01-29-2018 01:57 PM
[NMR paper] Functional Dynamics of Deuterated ?2 -Adrenergic Receptor in Lipid Bilayers Revealed by NMR Spectroscopy.
Functional Dynamics of Deuterated ?2 -Adrenergic Receptor in Lipid Bilayers Revealed by NMR Spectroscopy.
Related Articles Functional Dynamics of Deuterated ?2 -Adrenergic Receptor in Lipid Bilayers Revealed by NMR Spectroscopy.
Angew Chem Int Ed Engl. 2014 Oct 3;
Authors: Kofuku Y, Ueda T, Okude J, Shiraishi Y, Kondo K, Mizumura T, Suzuki S, Shimada I
Abstract
G-protein-coupled receptors (GPCRs) exist in conformational equilibrium between active and inactive states, and the former population determines the efficacy of...
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10-07-2014 02:31 PM
[NMR paper] An evaluation of detergents for NMR structural studies of membrane proteins.
An evaluation of detergents for NMR structural studies of membrane proteins.
Related Articles An evaluation of detergents for NMR structural studies of membrane proteins.
J Biomol NMR. 2004 Jan;28(1):43-57
Authors: Krueger-Koplin RD, Sorgen PL, Krueger-Koplin ST, Rivera-Torres IO, Cahill SM, Hicks DB, Grinius L, Krulwich TA, Girvin ME
Structural information on membrane proteins lags far behind that on soluble proteins, in large part due to difficulties producing homogeneous, stable, structurally relevant samples in a membrane-like environment....
Acetonitrile allows indirect replacement of non-deuterated lipid detergents by deuterated lipid detergents for the NMR study of detergent-soluble proteins
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