Phosphoserine (pSer) sites are primarily located within disordered protein regions, making it difficult to experimentally ascertain their effects on protein structure and function. Therefore, the production of ^(15)N (and ^(13)C)-labeled proteins with site-specifically encoded pSer for Nuclear Magnetic Resonance (NMR) studies is essential to uncover molecular mechanisms of protein regulation by phosphorylation. While genetic code expansion (GCE) technologies for the translational installation of...
[NMR paper] A genetically encoded 19F NMR probe for lysine acetylation.
A genetically encoded 19F NMR probe for lysine acetylation.
A genetically encoded 19F NMR probe for lysine acetylation.
Chem Commun (Camb). 2018 Mar 29;:
Authors: Zhang F, Zhou Q, Yang G, An L, Li F, Wang J
Abstract
Advances in acetylated protein-protein/DNA interactions depend on the development of a novel NMR (nuclear magnetic resonance) probe to study the conformational changes of acetylated proteins. However, the method for detecting the acetylated protein conformation is underdeveloped. Herein, an acetyllysine mimic has...
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03-30-2018 06:40 PM
[NMR paper] Genetically encoded amino acids with tert-butyl and trimethylsilyl groups for site-selective studies of proteins by NMR spectroscopy.
Genetically encoded amino acids with tert-butyl and trimethylsilyl groups for site-selective studies of proteins by NMR spectroscopy.
Genetically encoded amino acids with tert-butyl and trimethylsilyl groups for site-selective studies of proteins by NMR spectroscopy.
J Biomol NMR. 2017 Dec 02;:
Authors: Loh CT, Adams LA, Graham B, Otting G
Abstract
The amino acids 4-(tert-butyl)phenylalanine (Tbf) and 4-(trimethylsilyl)phenylalanine (TMSf), as well as a partially deuterated version of Tbf (dTbf), were chemically synthesized and...
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12-05-2017 07:35 PM
Genetically encoded amino acids with tert -butyl and trimethylsilyl groups for site-selective studies of proteins by NMR spectroscopy
Genetically encoded amino acids with tert -butyl and trimethylsilyl groups for site-selective studies of proteins by NMR spectroscopy
Abstract
The amino acids 4-(tert-butyl)phenylalanine (Tbf) and 4-(trimethylsilyl)phenylalanine (TMSf), as well as a partially deuterated version of Tbf (dTbf), were chemically synthesized and site-specifically incorporated into different proteins, using an amber stop codon, suppressor tRNA and the broadband aminoacyl-tRNA synthetase originally evolved for the incorporation of p-cyano-phenylalanine. The 1H-NMR signals of...
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12-02-2017 02:54 PM
[NMR paper] Expression and NMR Structural Studies of Isotopically Labeled Cannabinoid Receptor Type II.
Expression and NMR Structural Studies of Isotopically Labeled Cannabinoid Receptor Type II.
Related Articles Expression and NMR Structural Studies of Isotopically Labeled Cannabinoid Receptor Type II.
Methods Enzymol. 2017;593:387-403
Authors: Yeliseev A, Gawrisch K
Abstract
Cannabinoid receptor type II (CB2) is an integral membrane protein with seven transmembrane helices that belongs to the large superfamily of rhodopsin-like G protein-coupled receptors. The CB2 is a part of the endocannabinoid system that plays a vital role in...
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07-29-2017 10:35 AM
[NMR paper] A Genetically Encoded ?-Lactamase Reporter for Ultrasensitive (129) Xe NMR in Mammalian Cells.
A Genetically Encoded ?-Lactamase Reporter for Ultrasensitive (129) Xe NMR in Mammalian Cells.
Related Articles A Genetically Encoded ?-Lactamase Reporter for Ultrasensitive (129) Xe NMR in Mammalian Cells.
Angew Chem Int Ed Engl. 2016 Jun 15;
Authors: Wang Y, Roose BW, Palovcak EJ, Carnevale V, Dmochowski IJ
Abstract
Molecular imaging holds considerable promise for elucidating biological processes in normal physiology as well as disease states, but requires noninvasive methods for identifying analytes at sub-micromolar...
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06-16-2016 12:06 PM
[NMR paper] A Genetically Encoded 19 F NMR Probe for Tyrosine Phosphorylation.
A Genetically Encoded 19 F NMR Probe for Tyrosine Phosphorylation.
A Genetically Encoded 19 F NMR Probe for Tyrosine Phosphorylation.
Angew Chem Int Ed Engl. 2013 Feb 28;
Authors: Li F, Shi P, Li J, Yang F, Wang T, Zhang W, Gao F, Ding W, Li D, Li J, Xiong Y, Sun J, Gong W, Tian C, Wang J
Abstract
Simple and selective: Tyrosine phosphorylation is a pivotal post-translational modification which regulates the enzymatic activity, protein conformation, and protein-protein interactions. The highly efficient genetic incorporation of...
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03-02-2013 11:45 AM
Expression, purification and preliminary NMR characterization of isotopically labeled wild-type human heterotrimeric G protein ?i1
Expression, purification and preliminary NMR characterization of isotopically labeled wild-type human heterotrimeric G protein ?i1
August 2012
Publication year: 2012
Source:Protein Expression and Purification, Volume 84, Issue 2</br>
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Molecular-level investigation of proteins is increasingly important to researchers trying to understand the mechanisms of signal transmission. Heterotrimeric G proteins control the activation of many critical signal transmission cascades and are also implicated in numerous diseases. As part of a longer-term investigation of...
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02-03-2013 10:13 AM
Generation of Pseudocontact Shifts in Protein NMR Spectra with a Genetically Encoded
Generation of Pseudocontact Shifts in Protein NMR Spectra with a Genetically Encoded Cobalt(II)-Binding Amino Acid.
Related Articles Generation of Pseudocontact Shifts in Protein NMR Spectra with a Genetically Encoded Cobalt(II)-Binding Amino Acid.
Angew Chem Int Ed Engl. 2010 Nov 25;
Authors: Nguyen TH, Ozawa K, Stanton-Cook M, Barrow R, Huber T, Otting G
Accessing isotopically labeled proteins containing genetically encoded phosphoserine for NMR with optimized expression conditions
Linear Mode
