Related ArticlesAbsolute quantification of poly(dl-lactide-co-glycolide) in microspheres using quantitative 1H NMR spectroscopy.
J Pharm Biomed Anal. 2017 Nov 30;146:273-278
Authors: Zhang Q, Guo N, Sun Y, Li X, Yang H
Abstract
The complex nature and the manufacturing process of poly(dl-lactide-co-glycolide) (PLGA), a key component of PLGA-based microspheres, have made the quantification of this copolymer difficult. The main purpose of the current study was to investigate the potential of three different methods for the quantitative analysis of the PLGA content of clinical products. In this regard, leuprorelin acetate microspheres from different vendors were chosen as templates to validate quantitative 1H nuclear magnetic resonance (qHNMR) spectroscopy, size exclusion chromatography (SEC), and high-performance liquid chromatography (HPLC) methods qHNMR proved to be an excellent and rapid PLGA quantification method compared to the other two. The recovery value was 99.12% and the linearity correlation coefficient was 0.9999. The results obtained from the qHNMR method were found to match the data provided by the vendor, suggesting that qHNMR can be utilized as a reliable quality control and inspection tool for PLGA-based clinical products.
Protein delivery nanosystem of six-arm copolymer poly(ε-caprolactone)â??poly(ethylene glycol) for long-term sustained ... - Dove Medical Press
<img alt="" height="1" width="1">
Protein delivery nanosystem of six-arm copolymer poly(ε-caprolactone)â??poly(ethylene glycol) for long-term sustained ...
Dove Medical Press
Hydrogen-1 nuclear magnetic resonance spectrum, Fourier-transform infrared spectroscopy, and gel-permeation chromatography were conducted to identify the structure of 6S-PCL-PEG. The biocompatibility of the 6S-PCL-PEG was evaluated by a cell ...
Protein delivery nanosystem of six-arm copolymer poly(ε-caprolactone)â??poly(ethylene glycol) for long-term sustained ... - Dove Medical Press
More...
nmrlearner
Online News
0
05-08-2018 02:41 PM
[NMR paper] A simple and accurate protocol for absolute polar metabolite quantification in cell cultures using q-NMR.
A simple and accurate protocol for absolute polar metabolite quantification in cell cultures using q-NMR.
Related Articles A simple and accurate protocol for absolute polar metabolite quantification in cell cultures using q-NMR.
Anal Biochem. 2016 Feb 17;
Authors: Goldoni L, Beringhelli T, Rocchia W, Realini N, Piomelli D
Abstract
Absolute analyte quantification by NMR spectroscopy is rarely pursued in metabolomics, even though this would allow researchers to compare results obtained using different techniques. Here we report on...
Absolute quantification of poly(dl-lactide-co-glycolide) in microspheres using quantitative 1H NMR spectroscopy.
Linear Mode
