4D Non-uniformly sampled HCBCACON and 1J(NCÎ±)-selective HCBCANCO experiments for the sequential assignment and chemical shift analysis of intrinsically disordered proteins

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4D Non-uniformly sampled HCBCACON and 1J(NCÎ±)-selective HCBCANCO experiments for the sequential assignment and chemical shift analysis of intrinsically disordered proteins

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05-17-2012, 08:40 AM

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4D Non-uniformly sampled HCBCACON and 1J(NCÎ±)-selective HCBCANCO experiments for the sequential assignment and chemical shift analysis of intrinsically disordered proteins

4D Non-uniformly sampled HCBCACON and 1J(NCÎ±)-selective HCBCANCO experiments for the sequential assignment and chemical shift analysis of intrinsically disordered proteins

Abstract A pair of 4D NMR experiments for the backbone assignment of disordered proteins is presented. The experiments exploit 13C direct detection and non-uniform sampling of the indirectly detected dimensions, and provide correlations of the aliphatic proton (HÎ±, and HÎ²) and carbon (CÎ±, CÎ²) resonance frequencies to the protein backbone. Thus, all the chemical shifts regularly used to map the transient secondary structure motifs in the intrinsically disordered proteins (HÎ±, CÎ±, CÎ², Câ?², and N) can be extracted from each spectrum. Compared to the commonly used assignment strategy based on matching the CÎ± and CÎ² chemical shifts, inclusion of the HÎ± and HÎ² provides up to three extra resonance frequencies that decrease the chance of ambiguous assignment. The experiments were successfully applied to the original assignment of a 12.8 kDa intrinsically disordered protein having a high content of proline residues (26 %) in the sequence.

Content Type Journal Article
Category Article
Pages 1-10
DOI 10.1007/s10858-012-9631-8
Authors
- JiÅ?Ã* NovÃ¡Ä?ek, Faculty of Science, NCBR, and CEITEC, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
- Noam Y. Haba, Department of Chemistry, Bar Ilan University, Ramat Gan, 52900 Israel
- Jordan H. Chill, Department of Chemistry, Bar Ilan University, Ramat Gan, 52900 Israel
- LukÃ¡Å¡ Å½Ã*dek, Faculty of Science, NCBR, and CEITEC, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
- VladimÃ*r SklenÃ¡Å?, Faculty of Science, NCBR, and CEITEC, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic

- Journal Journal of Biomolecular NMR
- Online ISSN 1573-5001
- Print ISSN 0925-2738

Source: Journal of Biomolecular NMR

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