4,4-Difluoroproline as a Unique (19)F NMR Probe of Proline Conformation
 

4,4-Difluoroproline as a Unique (19)F NMR Probe of Proline Conformation

Despite the importance of proline conformational equilibria (trans versus cis amide and exo versus endo ring pucker) on protein structure and function, there is a lack of convenient ways to probe proline conformation. 4,4-Difluoroproline (Dfp) was identified to be a sensitive ^(19)F NMR-based probe of proline conformational biases and cis-trans isomerism. Within model compounds and disordered peptides, the diastereotopic fluorines of Dfp exhibit similar chemical shifts (??(FF) = 0-3 ppm) when a...

More...