Despite the importance of proline conformational equilibria (trans versus cis amide and exo versus endo ring pucker) on protein structure and function, there is a lack of convenient ways to probe proline conformation. 4,4-Difluoroproline (Dfp) was identified to be a sensitive ^(19)F NMR-based probe of proline conformational biases and cis-trans isomerism. Within model compounds and disordered peptides, the diastereotopic fluorines of Dfp exhibit similar chemical shifts (??(FF) = 0-3 ppm) when a...
Protein has unique effects on information processing - MIT News
Protein has unique effects on information processing - MIT News
Protein has unique effects on information processing MIT NewsMIT Picower Institute researchers find that a key protein linked to intellectual disability shapes electrical currents in neural connections.
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03-24-2019 10:41 PM
[NMR paper] A Unique Tool for Cellular Structural Biology: In-cell NMR.
A Unique Tool for Cellular Structural Biology: In-cell NMR.
Related Articles A Unique Tool for Cellular Structural Biology: In-cell NMR.
J Biol Chem. 2015 Dec 16;
Authors: Luchinat E, Banci L
Abstract
Conventional structural and chemical biology approaches are applied to macromolecules extrapolated from their native context. When doing so, important structural and functional features of macromolecules may be lost, which depend on their native network of interactions within the cell. In-cell nuclear magnetic resonance is a branch...
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12-28-2015 12:26 AM
[NMR paper] ?-(S)-Trifluoromethyl proline: evaluation as a structural substitute of proline for solid state (19)F-NMR peptide studies.
?-(S)-Trifluoromethyl proline: evaluation as a structural substitute of proline for solid state (19)F-NMR peptide studies.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.rsc.org-images-entities-char_z_RSClogo.gif Related Articles ?-(S)-Trifluoromethyl proline: evaluation as a structural substitute of proline for solid state (19)F-NMR peptide studies.
Org Biomol Chem. 2015 Mar 21;13(11):3171-81
Authors: Kubyshkin V, Afonin S, Kara S, Budisa N, Mykhailiuk PK, Ulrich AS
Abstract
...
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09-30-2015 10:34 PM
[NMR images] 1D 1H, pH 7.4 spectrum for L-Proline, bmse000047
http://bmrb.protein.osaka-u.ac.jp/metabolomics/standards/L_proline/nmr/bmse000047/spectra_png/1H/00.png
8/04/2014 7:46:05 AM GMT
1D 1H, pH 7.4 spectrum for L-Proline, bmse000047
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[Question from NMRWiki Q&A forum] Tuning probe failed after a dual probe was replaced with a BBI probe
Tuning probe failed after a dual probe was replaced with a BBI probe
We generally use Dual to run 13C and BBI to run 2D. After changed the probe, the command "edhead" was used to set the probe. Put the sample tube, lock the solvent, and then type "atma" to tune the probe. We always do it like this, but now we can not tune the proton after installed the BBI probe (13C is OK). The dip can not be found by "atma", and "atmm" was also not work on forming a dip. What is the most possible reason for this error? How to solve it and avoid it in the future ? Thanks. (Instrument: Bruker 400 MHz,...
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08-23-2011 05:31 PM
[NMR paper] Proline pipe helix: structure of the tus proline repeat determined by 1H NMR.
Proline pipe helix: structure of the tus proline repeat determined by 1H NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Proline pipe helix: structure of the tus proline repeat determined by 1H NMR.
Biochemistry. 1996 Jan 23;35(3):698-703
Authors: Butcher DJ, Nedved ML, Neiss TG, Moe GR
The structure of a 22 amino acid peptide, TPPI , that is similar to the proline repeat segment of the replication arrest protein, Tus, has been determined by 1H NMR in 50% trifluroethanol. The...
4,4-Difluoroproline as a Unique (19)F NMR Probe of Proline Conformation
Linear Mode
