[NMR paper] A 3D MAS NMR experiment utilizing through-space 15N-15N correlations.
A 3D MAS NMR experiment utilizing through-space 15N-15N correlations.
Related Articles A 3D MAS NMR experiment utilizing through-space 15N-15N correlations.
J Am Chem Soc. 2017 Apr 27;:
Authors: Donovan KJ, Silvers R, Linse S, Griffin RG
Abstract
We demonstrate a novel 3D NNC magic angle spinning (MAS) NMR experiment that generates 15N- 15N internuclear contacts in protein systems using an optimized 15N- 15N proton assisted recoupling (PAR) mixing period and a 13C dimension for improved resolution. The optimized PAR...
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04-28-2017 02:27 PM
Space Race: Inside a high-stakes experiment in protein crystallization - UAB News
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Space Race: Inside a high-stakes experiment in protein crystallization
UAB News
(The other is nuclear magnetic resonance spectroscopy.) Crystallography starts with crystals â?? comprising hundreds of thousands of protein molecules, packed together like a three-dimensional wall of bricks. By bombarding a crystal with X-rays, and ...
Space Race: Inside a high-stakes experiment in protein crystallization - UAB...
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04-13-2015 09:11 PM
[NMR paper] 3D 15N/15N/1H Chemical Shift Correlation Experiment Utilizing an RFDR-based 1H/1H Mixing Period at 100 kHz MAS
3D 15N/15N/1H Chemical Shift Correlation Experiment Utilizing an RFDR-based 1H/1H Mixing Period at 100 kHz MAS
Publication date: Available online 19 April 2014
Source:Journal of Magnetic Resonance</br>
Author(s): Yusuke Nishiyama , Michal Malon , Yuji Ishii , Ayyalusamy Ramamoorthy</br>
Homonuclear correlation NMR experiments are commonly used in the high-resolution structural studies of proteins. While 13C/13C chemical shift correlation experiments utilizing dipolar recoupling techniques are fully utilized under MAS, correlation of the chemical shifts of 15N...
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04-20-2014 07:58 AM
[Question from NMRWiki Q&A forum] Sensitivity gain by utilizing steady state polarization
Sensitivity gain by utilizing steady state polarization
What do we exactly mean by "utilizing steady state polarization" to gain sensitivity in an experiment?...I know that steady state should be achieved before the start of acquisition which means, in a way, it is already being used for 1H,13C/15N(isn't it?)...I am lost in understanding this concept!...also, how/why does it differ in standard pulse sequences and spin state selective coherence transfer experiments?
Check if somebody has answered this question on NMRWiki QA forum
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08-05-2013 09:06 AM
[NMR paper] Utilizing NMR and EPR spectroscopy to probe the role of copper in prion diseases.
Utilizing NMR and EPR spectroscopy to probe the role of copper in prion diseases.
Utilizing NMR and EPR spectroscopy to probe the role of copper in prion diseases.
Magn Reson Chem. 2013 Feb 24;
Authors: Emwas AH, Al-Talla ZA, Guo X, Al-Ghamdi S, Al-Masri HT
Abstract
Copper is an essential nutrient for the normal development of the brain and nervous system, although the hallmark of several neurological diseases is a change in copper concentrations in the brain and central nervous system. Prion protein (PrP) is a copper-binding,...
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02-26-2013 06:35 PM
An NMR strategy for fragment-based ligand screening utilizing a paramagnetic lanthanide probe
An NMR strategy for fragment-based ligand screening utilizing a paramagnetic lanthanide probe
Abstract A nuclear magnetic resonance-based ligand screening strategy utilizing a paramagnetic lanthanide probe is presented. By fixing a paramagnetic lanthanide ion to a target protein, a pseudo-contact shift (PCS) and a paramagnetic relaxation enhancement (PRE) can be observed for both the target protein and its bound ligand. Based on PRE and PCS information, the bound ligand is then screened from the compound library and the structure of the ligandâ??protein complex is determined. PRE is an...
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09-20-2011 05:02 AM
[Stan NMR blog] Is every finite-dimensional Hilbert space a spin-space?
Is every finite-dimensional Hilbert space a spin-space?
Every linear operator on any finite Hilbert space can be generated from spin operators.
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08-21-2010 06:14 PM
Precise structural determination of weakly binding peptides by utilizing dihedral ang
Abstract Structural determination of target-bound conformations of peptides is of primary importance for the optimization of peptide ligands and peptideâ??mimetic design. In the structural determination of weakly binding ligands, transferred nuclear Overhauser effect (TrNOE) methods have been widely used. However, not many distance constraints can be obtained from small peptide ligands by TrNOE, especially for peptides bound to a target molecule in an extended conformation. Therefore, for precise structural determination of weakly binding peptides, additional structural constraints are...
3D MASNMR Experiment Utilizing Through-Space 15N–15NCorrelations
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