Abstract
We demonstrate a novel 3D NNC magic angle spinning (MAS) NMR experiment that generates 15N- 15N internuclear contacts in protein systems using an optimized 15N- 15N proton assisted recoupling (PAR) mixing period and a 13C dimension for improved resolution. The optimized PAR condition permits the acquisition of high signal-to-noise 3D data that enables backbone chemical shift assignments using a strategy that is complementary to current schemes. The spectra can also provide distance constraints. The utility of the experiment is demonstrated on an M0Ab1-42 fibril sample that yields high-quality data that is readily assigned and interpreted. The 3D NNC experiment therefore provides a powerful platform for solid-state protein studies and is broadly applicable to a variety of systems and experimental conditions.
PMID: 28447786 [PubMed - as supplied by publisher]
HN-NCA heteronuclear TOCSY-NH experiment for 1 H N and 15 N sequential correlations in ( 13 C, 15 N) labelled intrinsically disordered proteins
HN-NCA heteronuclear TOCSY-NH experiment for 1 H N and 15 N sequential correlations in ( 13 C, 15 N) labelled intrinsically disordered proteins
Abstract
A simple triple resonance NMR experiment that leads to the correlation of the backbone amide resonances of each amino acid residue â??iâ?? with that of residues â??iâ??1â?? and â??i+1â?? in (13C, 15N) labelled intrinsically disordered proteins (IDPs) is presented. The experimental scheme, {HN-NCA heteronuclear TOCSY-NH}, exploits the favourable relaxation properties of IDPs and the presence of 1 ...
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08-18-2015 10:07 AM
Space Race: Inside a high-stakes experiment in protein crystallization - UAB News
http://www.bionmr.com//t1.gstatic.com/images?q=tbn:ANd9GcTdLSDTqT9KHcynbDyRGfWeKnEzt1ralm67yDa3WwS2dRd9txZmssQcRxq5L2_rgLOrksdK31E
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Space Race: Inside a high-stakes experiment in protein crystallization
UAB News
(The other is nuclear magnetic resonance spectroscopy.) Crystallography starts with crystals â?? comprising hundreds of thousands of protein molecules, packed together like a three-dimensional wall of bricks. By bombarding a crystal with X-rays, and ...
Space Race: Inside a high-stakes experiment in protein crystallization - UAB...
[NMR paper] 3D 15N/15N/1H Chemical Shift Correlation Experiment Utilizing an RFDR-based 1H/1H Mixing Period at 100 kHz MAS
3D 15N/15N/1H Chemical Shift Correlation Experiment Utilizing an RFDR-based 1H/1H Mixing Period at 100 kHz MAS
Publication date: Available online 19 April 2014
Source:Journal of Magnetic Resonance</br>
Author(s): Yusuke Nishiyama , Michal Malon , Yuji Ishii , Ayyalusamy Ramamoorthy</br>
Homonuclear correlation NMR experiments are commonly used in the high-resolution structural studies of proteins. While 13C/13C chemical shift correlation experiments utilizing dipolar recoupling techniques are fully utilized under MAS, correlation of the chemical shifts of 15N...
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04-20-2014 07:58 AM
[NMR paper] A NMR experiment for simultaneous correlations of valine and leucine/isoleucine methyls with carbonyl chemical shifts in proteins.
A NMR experiment for simultaneous correlations of valine and leucine/isoleucine methyls with carbonyl chemical shifts in proteins.
A NMR experiment for simultaneous correlations of valine and leucine/isoleucine methyls with carbonyl chemical shifts in proteins.
J Biomol NMR. 2013 Dec 18;
Authors: Tugarinov V, Venditti V, Marius Clore G
Abstract
A methyl-detected 'out-and-back' NMR experiment for obtaining simultaneous correlations of methyl resonances of valine and isoleucine/leucine residues with backbone carbonyl chemical shifts,...
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12-19-2013 06:38 PM
[Question from NMRWiki Q&A forum] From what experiment only two bond correlations we can get in varian nmr?
From what experiment only two bond correlations we can get in varian nmr?
Hi., i have one compound i need only two bond correlation contours from quaternary carbon to proton. Is there any pulse sequence to find only two bond correlations ie, from quaternary carbon to protons with minimum amount of sample in "varian nmr".
Check if somebody has answered this question on NMRWiki QA forum
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12-28-2010 05:27 AM
[NMR paper] 1H(C) and 1H(N) total NOE correlations in a single 3D NMR experiment. 15N and 13C tim
1H(C) and 1H(N) total NOE correlations in a single 3D NMR experiment. 15N and 13C time-sharing in t1 and t2 dimensions for simultaneous data acquisition.
Related Articles 1H(C) and 1H(N) total NOE correlations in a single 3D NMR experiment. 15N and 13C time-sharing in t1 and t2 dimensions for simultaneous data acquisition.
J Biomol NMR. 2003 Nov;27(3):193-203
Authors: Xia Y, Yee A, Arrowsmith CH, Gao X
Simultaneous data acquisition in time-sharing (TS) multi-dimensional NMR experiments has been shown an effective means to reduce experimental...
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11-24-2010 09:16 PM
Simultaneous detection of amide and methyl correlations using a time shared NMR experiment: application to binding epitope mapping
Simultaneous detection of amide and methyl correlations using a time shared NMR experiment: application to binding epitope mapping
Peter Würtz, Olli Aitio, Maarit Hellman and Perttu Permi
Journal of Biomolecular NMR; 2007; 39(2) pp 97 - 105
Abstract:
Simultaneous recording of different NMR parameters is an efficient way to reduce the overall experimental time and speed up structural studies of biological macromolecules. This can especially be beneficial in the case of fast NMR-based drug screening applications or for collecting NOE restraints, where prohibitively long data collection...
A 3D MAS NMR experiment utilizing through-space 15N-15N correlations.
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