Related ArticlesA 31P NMR study of mitochondrial inorganic phosphate visibility: effects of Ca2+, Mn2+, and the pH gradient.
Biochemistry. 1992 Feb 11;31(5):1322-30
Authors: Hutson SM, Williams GD, Berkich DA, LaNoue KF, Briggs RW
The effects of external pH, temperature, and Ca2+ and Mn2+ concentrations on the compartmentation and NMR visibility of inorganic phosphate (Pi) were studied in isolated rat liver mitochondria respiring on succinate and glutamate. Mitochondrial matrix Pi is totally visible by NMR at 8 degrees C and at low external concentrations of Pi. However, when the external Pi concentration is increased above 7 mM, the pH gradient decreases, the amount of matrix Pi increases, and the fraction not observed by NMR increases. Raising the temperature to 25 degrees C also decreases the pH gradient and the Pi fraction observed by NMR. At physiologically relevant concentrations, Ca2+ and Mn2+ do not seem to play a major role in matrix Pi NMR invisibility. For Ca2+ concentrations above 30 nmol/mg of protein, formation of insoluble complexes will cause loss of Pi signal intensity. For Mn2+ concentrations above 2 nmol/mg of protein, the Pi peak can be broadened sufficiently to preclude detection of a high-resolution signal. The results indicate that mitochondrial matrix Pi should be mostly observable up to 25 degrees C by high-resolution NMR. While the exact nature of the NMR-invisible phosphate in perfused or in vivo liver is yet to be determined, better success at detecting and resolving both Pi pools by NMR is indicated at high field, low temperature, and optimized pulsing conditions.
Postdoctoral teaching fellowship in NMR methodology for the study of inorganic materials
Postdoctoral teaching fellowship in NMR methodology for the study of inorganic materials
A postdoctoral teaching fellowship in NMR methodology is available in the ENSCL (Lille, France). It will start in September 2011. This position may be turned into a permanent position of assistant professor in Lille after a training period.
You will join the team of Glasses and NMR methodology in Lille NMR center. You will contribute to the development of original NMR methods for the study of glasses and catalysts. In particular you will be expected to develop techniques for the observation of...
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Study of acute biochemical effects of thallium toxicity in mouse urine by NMR spectroscopy.
Study of acute biochemical effects of thallium toxicity in mouse urine by NMR spectroscopy.
Study of acute biochemical effects of thallium toxicity in mouse urine by NMR spectroscopy.
J Appl Toxicol. 2011 Jan 7;
Authors: Tyagi R, Rana P, Khan AR, Bhatnagar D, Devi MM, Chaturvedi S, Tripathi RP, Khushu S
Thallium (Tl) is a toxic heavy metal and its exposure to the human body causes physiological and biochemical changes due to its interference with potassium-dependent biological reactions. A high-resolution (1) H NMR spectroscopy based metabonomic...
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01-11-2011 11:27 PM
[NMR paper] Phosphate ions in bone: identification of a calcium-organic phosphate complex by 31P
Phosphate ions in bone: identification of a calcium-organic phosphate complex by 31P solid-state NMR spectroscopy at early stages of mineralization.
Related Articles Phosphate ions in bone: identification of a calcium-organic phosphate complex by 31P solid-state NMR spectroscopy at early stages of mineralization.
Calcif Tissue Int. 2003 May;72(5):610-26
Authors: Wu Y, Ackerman JL, Strawich ES, Rey C, Kim HM, Glimcher MJ
Previous 31P cross-polarization and differential cross-polarization magic angle spinning (CP/MAS and DCP/MAS) solid-state NMR...
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11-24-2010 09:01 PM
[NMR paper] 13C NMR study of the effects of mutation on the tryptophan dynamics in chymotrypsin i
13C NMR study of the effects of mutation on the tryptophan dynamics in chymotrypsin inhibitor 2: correlations with structure and stability.
Related Articles 13C NMR study of the effects of mutation on the tryptophan dynamics in chymotrypsin inhibitor 2: correlations with structure and stability.
Biochemistry. 1993 Jan 19;32(2):657-62
Authors: Matthews SJ, Jandu SK, Leatherbarrow RJ
Recombinant chymotrypsin inhibitor 2 (CI-2) and the three mutants Ile39-->Val, Ile39-->Leu, and Arg67-->Ala were successfully enriched with tryptophan at position...
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08-21-2010 11:53 PM
[NMR paper] NMR study of the phosphate-binding elements of Escherichia coli elongation factor Tu
NMR study of the phosphate-binding elements of Escherichia coli elongation factor Tu catalytic domain.
Related Articles NMR study of the phosphate-binding elements of Escherichia coli elongation factor Tu catalytic domain.
Biochemistry. 1991 Nov 12;30(45):10872-7
Authors: Lowry DF, Cool RH, Redfield AG, Parmeggiani A
The phosphoryl-binding elements in the GDP-binding domain of elongation factor Tu were studied by heteronuclear proton observe methods. Five proton resonances were found below 10.5 ppm. Two of these were assigned to the amide...
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[NMR paper] NMR study of the phosphate-binding elements of Escherichia coli elongation factor Tu
NMR study of the phosphate-binding elements of Escherichia coli elongation factor Tu catalytic domain.
Related Articles NMR study of the phosphate-binding elements of Escherichia coli elongation factor Tu catalytic domain.
Biochemistry. 1991 Nov 12;30(45):10872-7
Authors: Lowry DF, Cool RH, Redfield AG, Parmeggiani A
The phosphoryl-binding elements in the GDP-binding domain of elongation factor Tu were studied by heteronuclear proton observe methods. Five proton resonances were found below 10.5 ppm. Two of these were assigned to the amide...
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08-21-2010 11:12 PM
[NMR paper] 31P NMR saturation-transfer study of the in situ kinetics of the mitochondrial adenin
31P NMR saturation-transfer study of the in situ kinetics of the mitochondrial adenine nucleotide translocase.
Related Articles 31P NMR saturation-transfer study of the in situ kinetics of the mitochondrial adenine nucleotide translocase.
Biochemistry. 1991 Aug 27;30(34):8351-7
Authors: Masiakos PT, Williams GD, Berkich DA, Smith MB, LaNoue KF
The exchange of intramitochondrial ATP (ATP(in)) for extramitochondrial ATP (ATP(out)) was measured by using 31P NMR spectroscopy over a range of temperatures in isolated rat liver mitochondria oxidizing...
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08-21-2010 11:12 PM
[NMR paper] 31P NMR saturation-transfer study of the in situ kinetics of the mitochondrial adenin
31P NMR saturation-transfer study of the in situ kinetics of the mitochondrial adenine nucleotide translocase.
Related Articles 31P NMR saturation-transfer study of the in situ kinetics of the mitochondrial adenine nucleotide translocase.
Biochemistry. 1991 Aug 27;30(34):8351-7
Authors: Masiakos PT, Williams GD, Berkich DA, Smith MB, LaNoue KF
The exchange of intramitochondrial ATP (ATP(in)) for extramitochondrial ATP (ATP(out)) was measured by using 31P NMR spectroscopy over a range of temperatures in isolated rat liver mitochondria oxidizing...
A 31P NMR study of mitochondrial inorganic phosphate visibility: effects of Ca2+, Mn2
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