Related Articles31P NMR studies of ATP concentrations and Pi-ATP exchange in the rat kidney in vivo: effects of inhibiting and stimulating renal metabolism.
Magn Reson Med. 1990 Jun;14(3):445-60
Authors: Shine N, Xuan A, Weiner MW
Previous investigators found that cyanide (CN-) is a potent inhibitor of renal Na+ transport, while the uncoupling agent 2,4-dinitrophenol (DNP) and fructose (both which lower ATP levels) are weak transport inhibitors. To examine the disparate effects of these substances measurements of ATP were performed, using 31P NMR, while simultaneously monitoring renal Na+ transport. Infusion of CN-, DNP, and fructose lowered whole kidney ATP levels by about the same extent (30%) while only CN- inhibited Na+ transport. This may be due to the fact that CN- has a potent action on the thick ascending limb of Henle, while fructose and DNP may have a more proximal action. Alternatively, ATP turnover may be a more important determinant of transport than ATP concentrations. Saturation transfer experiments were performed to measure Pi-ATP flux. Unilateral nephrectomy, high protein feeding, and methylprednisolone were used to stimulate metabolism and transport. The rate of Pi-ATP flux was 20.1 mumol/min/g. However, because oxygen consumption was stimulated, the ATP/O ratio was 0.85, considerably less than the theoretical value of 3. Finally, atrial natriuretic factor, which increased Na+ transport, had no effect on Pi-ATP flux. The results raise the possibility that the saturation transfer technique does not detect all Pi-ATP flux, especially when renal metabolism is stimulated.
Detection of autosomal dominant polycystic kidney disease by NMR spectroscopic fingerprinting of urine.
Detection of autosomal dominant polycystic kidney disease by NMR spectroscopic fingerprinting of urine.
Detection of autosomal dominant polycystic kidney disease by NMR spectroscopic fingerprinting of urine.
Kidney Int. 2011 Mar 9;
Authors: Gronwald W, Klein MS, Zeltner R, Schulze BD, Reinhold SW, Deutschmann M, Immervoll AK, Böger CA, Banas B, Eckardt KU, Oefner PJ
Autosomal dominant polycystic kidney disease (ADPKD) is a frequent cause of kidney failure; however, urinary biomarkers for the disease are lacking. In a step towards identifying such...
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[NMR paper] NMR studies on Cu(II)-peptide complexes: exchange kinetics and determination of struc
NMR studies on Cu(II)-peptide complexes: exchange kinetics and determination of structures in solution.
Related Articles NMR studies on Cu(II)-peptide complexes: exchange kinetics and determination of structures in solution.
Mol Biosyst. 2005 May;1(1):79-84
Authors: Gaggelli E, Kozlowski H, Valensin D, Valensin G
The interaction of copper(II) with histidine containing peptides has recently acquired renewed interest following the established link between abnormal protein behaviour in neurodegenerative processes and unpaired copper homeostasis....
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[NMR paper] Sub-second 2D NMR spectroscopy at sub-millimolar concentrations.
Sub-second 2D NMR spectroscopy at sub-millimolar concentrations.
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J Am Chem Soc. 2004 Sep 29;126(38):11756-7
Authors: Shapira B, Morris E, Muszkat KA, Frydman L
A recently proposed protocol enables the acquisition of two-dimensional nuclear magnetic resonance (2D NMR) spectra within a single scan. A promising application opened up by this new data acquisition mode concerns its combination with active nuclear polarization methods, whereby spectroscopy is carried...
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[NMR paper] Mutational, NMR, and NH exchange studies of the tight and selective binding of 8-oxo-
Mutational, NMR, and NH exchange studies of the tight and selective binding of 8-oxo-dGMP by the MutT pyrophosphohydrolase.
Related Articles Mutational, NMR, and NH exchange studies of the tight and selective binding of 8-oxo-dGMP by the MutT pyrophosphohydrolase.
Biochemistry. 2004 Mar 30;43(12):3404-14
Authors: Saraswat V, Azurmendi HF, Mildvan AS
The solution structure of the ternary MutT enzyme-Mg(2+)-8-oxo-dGMP complex showed the proximity of Asn119 and Arg78 and the modified purine ring of 8-oxo-dGMP, suggesting specific roles for...
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[Question from NMRWiki Q&A forum] Which are better methods of water suppression at higher salt concentrations?
Which are better methods of water suppression at higher salt concentrations?
Hello, could anyone recommend better technique for water suppression for the samples having higher salt concentration?
At what salt concentration is the method effective?
Dr. Talluri mentioned in his post that water suppression using soft pulses (such as one using excitation sculpting) is not as friendly for high salt samples - what is the mechanism?
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08-22-2010 02:30 AM
[NMR paper] In vivo 13C and 15N NMR studies of methylamine metabolism in Pseudomonas species MA.
In vivo 13C and 15N NMR studies of methylamine metabolism in Pseudomonas species MA.
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J Biol Chem. 1991 Jun 25;266(18):11705-13
Authors: Jones JG, Bellion E
Pseudomonas species MA was grown with methylamine as a sole source of carbon and nitrogen enabling the total flow of carbon and nitrogen into this organism to be simultaneously monitored in vivo using 13C and 15N NMR. Methylamine was rapidly and extensively incorporated into the methyl...
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Lipoprotein size, concentrations associated with incident type 2 diabetes - Endocrine
Lipoprotein size, concentrations associated with incident type 2 diabetes - Endocrine Today
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Lipoprotein size, concentrations associated with incident type 2 diabetes
Endocrine Today
Mora and colleagues used nuclear magnetic resonance spectroscopy on a small blood specimen to measure baseline lipoprotein particle size and concentration, ...
31P NMR studies of ATP concentrations and Pi-ATP exchange in the rat kidney in vivo:
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