Related Articles31P NMR examination of phosphorus metabolites in the aqueous, acidic, and organic extracts of Phaseolus vulgaris seeds.
Anal Biochem. 1993 Feb 15;209(1):85-94
Authors: Crans DC, Mikus M, Marshman RW
31P NMR spectroscopy was used to compare the phosphorus compound content in aqueous, acidic, and organic extracts of Phaseolus vulgaris seeds. Two chloroform-methanol and three ethanol extractions were used to isolate phospholipids from dry cotyledons and the phospholipid profiles were compared. Variations in phospholipid composition and artificially high concentrations of lysophosphatidylcholine were observed in the ethanol extracts. Other phosphorus compounds were extracted using perchloric acid, trichloroacetic acid, trichloroacetic acid in ether, hydrochloric acid, boiling water, and aqueous Hepes. The Hepes extraction was introduced in order to compare the results of a gentle procedure with those involving heat and acid treatments. Low concentrations of phospho-sugars and other phosphorus metabolites were found in all these extracts. High concentrations of phytate were found in all aqueous and acidic extractions; however, 31P NMR spectra of aqueous extractions did not show the phytate resonance. The NMR silence of the phytate resonances is attributed to complexation of phytate. The 31P NMR spectra of aqueous extracts contained a broad resonance at 0.1 ppm assigned to protein-bound RNA. Various extraction procedures on the same biological material are presented, and the comparison of these methods will facilitate future analysis and interpretation of literature reports involving these methods of tissue analysis.
(1)H NMR study of monocrotaline and its metabolites in human blood.
(1)H NMR study of monocrotaline and its metabolites in human blood.
(1)H NMR study of monocrotaline and its metabolites in human blood.
Food Chem Toxicol. 2011 Aug 6;
Authors: Yang YC, Crowder J, Wardle NJ, Yang L, White KN, Wang ZT, Annie Bligh SW
Monocrotaline (MCT) is a naturally occurring hepatotoxic pyrrolizidine alkaloid found in plants. This investigation is aimed at furthering the understanding of the role of blood in mediating the transport of MCT and its reactive metabolites in humans. Reactions of monocrotaline and its metabolites,...
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[NMR paper] Reinvestigation by phosphorus NMR of lipid distribution in bicelles.
Reinvestigation by phosphorus NMR of lipid distribution in bicelles.
Related Articles Reinvestigation by phosphorus NMR of lipid distribution in bicelles.
Biophys J. 2005 Mar;88(3):1887-901
Authors: Triba MN, Warschawski DE, Devaux PF
Mixtures of dimyristoyl-phosphatidylcholine (DMPC) and dihexanoyl-phosphatidylcholine (DHPC) in water form disks also called bicelles and different bilayer organizations when the mol ratio of the two lipids and the temperature are varied. The spontaneous alignment in a magnetic field of these bilayers above the...
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[NMR paper] Characterization of intracellular metabolites of axenic amastigotes of Leishmania don
Characterization of intracellular metabolites of axenic amastigotes of Leishmania donovani by 1H NMR spectroscopy.
Related Articles Characterization of intracellular metabolites of axenic amastigotes of Leishmania donovani by 1H NMR spectroscopy.
Acta Trop. 1999 Jul 30;73(2):121-33
Authors: Gupta N, Goyal N, Singha UK, Bhakuni V, Roy R, Rastogi AK
The intracellular metabolites of long-term in vitro cultured axenic amastigotes of Leishmania donovani (strain Dd8) were determined and compared with those of promastigotes and intracellular...
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11-18-2010 08:31 PM
Evolution of organic matter during composting of different organic wastes assessed by
Evolution of organic matter during composting of different organic wastes assessed by CPMAS (13)C NMR spectroscopy.
Related Articles Evolution of organic matter during composting of different organic wastes assessed by CPMAS (13)C NMR spectroscopy.
Waste Manag. 2010 Oct 19;
Authors: Caricasole P, Provenzano MR, Hatcher PG, Senesi N
In this paper, the evolution of organic matter (OM) during composting of different mixtures of various organic wastes was assessed by means of chemical analyses and CPMAS (13)C NMR spectroscopy measured during...
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A quantitative NMR spectroscopic examination of the flexibility of the C-terminal ext
A quantitative NMR spectroscopic examination of the flexibility of the C-terminal extensions of the molecular chaperones, alphaA- and alphaB-crystallin.
Related Articles A quantitative NMR spectroscopic examination of the flexibility of the C-terminal extensions of the molecular chaperones, alphaA- and alphaB-crystallin.
Exp Eye Res. 2010 Aug 20;
Authors: Treweek TM, Rekas A, Walker MJ, Carver JA
The principal lens proteins alphaA- and alphaB-crystallin are members of the small heat-shock protein (sHsp) family of molecular chaperone proteins. Via...
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[NMR paper] The structure of apo-calmodulin. A 1H NMR examination of the carboxy-terminal domain.
The structure of apo-calmodulin. A 1H NMR examination of the carboxy-terminal domain.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles The structure of apo-calmodulin. A 1H NMR examination of the carboxy-terminal domain.
FEBS Lett. 1993 Dec 27;336(2):368-74
Authors: Finn BE, Drakenberg T, Forsén S
The structure of the carboxy-terminal domain of bovine calmodulin, TR2C, in the calcium-free form was investigated using two-dimensional 1H NMR. Sequential resonance...
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[NMR paper] An in vitro kinetic assay of ATPase by phosphorus-31 NMR.
An in vitro kinetic assay of ATPase by phosphorus-31 NMR.
Related Articles An in vitro kinetic assay of ATPase by phosphorus-31 NMR.
Kurume Med J. 1990;37(3):153-7
Authors: Chinami M, Shingu M
The ATPase activity of RecA protein was examined by monitoring the changes of NMR phosphorus signals of ATP, ADP and inorganic phosphate. The areas of phosphorus-31 NMR peaks from inorganic phosphate and ADP, which increased with time, and the signals from ATP, which decreased with time, were fitted by a linear least square method to obtain the initial...
31P NMR examination of phosphorus metabolites in the aqueous, acidic, and organic ext
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