Related Articles31P-CP-MAS NMR studies on TPP+ bound to the ion-coupled multidrug transport protein EmrE.
FEBS Lett. 2000 Sep 1;480(2-3):127-31
Authors: Glaubitz C, Gröger A, Gottschalk K, Spooner P, Watts A, Schuldiner S, Kessler H
The binding of tetraphenylphosphonium (TPP+) to EmrE, a membrane-bound, 110 residue Escherichia coli multidrug transport protein, has been observed by 31P cross-polarisation-magic-angle spinning nuclear magnetic resonance spectroscopy (CP-MAS NMR). EmrE has been reconstituted into dimyristoyl phosphatidylcholine bilayers. CP-MAS could selectively distinguish binding of TPP+ to EmrE in the fluid membrane. A population of bound ligand appears shifted 4 ppm to lower frequency compared to free ligand in solution, which suggests a rather direct and specific type of interaction of the ligand with the protein. This is also supported by the observed restricted motion of the bound ligand. The observation of another weakly bound substrate population arises from ligand binding to negatively charged residues in the protein loop regions.
Transferred NOESY NMR studies of biotin mimetic peptide (FSHPQNT) bound to streptavidin: A structural model for studies of peptide-protein interactions.
Transferred NOESY NMR studies of biotin mimetic peptide (FSHPQNT) bound to streptavidin: A structural model for studies of peptide-protein interactions.
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Chem Biol Drug Des. 2011 Feb 5;
Authors: Gizachew D, Dratz E
Protein-protein interactions control signaling, specific adhesion and many other biological functions. The three dimensional structures of the interfaces and bound ligand can be...
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[NMR paper] NMR studies of the phosphorylation motif of the HIV-1 protein Vpu bound to the F-box
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Biochemistry. 2003 Dec 23;42(50):14741-51
Authors: Coadou G, Gharbi-Benarous J, Megy S, Bertho G, Evrard-Todeschi N, Segeral E, Benarous R, Girault JP
A protein-protein association regulated by phosphorylation of serine is examined by NMR studies. Degradation of the HIV receptor CD4 by the proteasome, mediated by the HIV-1...
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[NMR paper] 13C/31P NMR studies on the role of glucose transport/phosphorylation in human glycoge
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Int J Sports Med. 2003 May;24(4):238-44
Authors: Price TB, Laurent D, Petersen KF
This study measured muscle glycogen during a 7-day carbohydrate loading protocol. Twenty healthy subjects (12 male, 8 female) performed 1 hr treadmill/toe-raise exercise immediately before a 3-day low carbohydrate (LoCHO) diet (20 %...
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[NMR paper] NMR studies of novel inhibitors bound to farnesyl-protein transferase.
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http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles NMR studies of novel inhibitors bound to farnesyl-protein transferase.
Protein Sci. 1995 Apr;4(4):681-8
Authors: Koblan KS, Culberson JC, Desolms SJ, Giuliani EA, Mosser SD, Omer CA, Pitzenberger SM, Bogusky...
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J Med Chem. 1992 Jun 26;35(13):2467-73
Authors: Petros AM, Gemmecker G, Neri P, Olejniczak ET, Nettesheim D, Xu RX, Gubbins EG, Smith H, Fesik SW
Multidimensional, heteronuclear NMR methods were used to determine the complete 1H and 13C resonance assignments for ascomycin bound to recombinant FKBP, including stereospecific assignment of all 22 methylene protons. The...
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[NMR paper] NMR studies of [U-13C]cyclosporin A bound to cyclophilin: bound conformation and port
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Biochemistry. 1991 Jul 2;30(26):6574-83
Authors: Fesik SW, Gampe RT, Eaton HL, Gemmecker G, Olejniczak ET, Neri P, Holzman TF, Egan DA, Edalji R, Simmer R
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[NMR paper] 15N and 13C NMR studies of ligands bound to the 280,000-dalton protein porphobilinoge
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Biochemistry. 1990 Sep 11;29(36):8345-50
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31P-CP-MAS NMR studies on TPP+ bound to the ion-coupled multidrug transport protein E
Linear Mode
