Related ArticlesA 2H solid-state NMR spectroscopic investigation of biomimetic bicelles containing cholesterol and polyunsaturated phosphatidylcholine.
Chem Phys Lipids. 2004 Nov;132(1):55-64
Authors: Minto RE, Adhikari PR, Lorigan GA
Deuterium solid-state NMR spectroscopy was used to qualitatively study the effects of both 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphatidylcholine (PLiPC) and cholesterol on magnetically aligned phospholipid bilayers (bicelles) as a function of temperature utilizing the chain-perdeuterated probe 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC-d54) in DMPC/dihexanoylPC (DHPC) phospholipid bilayers. The results demonstrate that polyunsaturated PC and cholesterol were successfully incorporated into DMPC/DHPC phospholipid bilayers, leading to a bicelle that will be useful for investigations of eukaryotic membrane protein-lipid interactions. The data indicate that polyunsaturated PC increases membrane fluidity and decreases the minimum magnetic alignment temperature for DMPC/DHPC bicelles. Conversely, the introduction of cholesterol into aligned DMPC/DHPC bilayers decreases fluidity in the membrane and increases the minimum temperature necessary to magnetically align the phospholipid bilayers. Finally, the addition of Tm3+ to magnetically aligned DMPC/DMPC-d54/PLiPC/DHPC bilayers doubles the quadrupolar splittings, indicating that this unique bicelle system can be aligned with the bilayer normal parallel to the static magnetic field.
Long-Term-Stable Ether-Lipid vs Conventional Ester-Lipid Bicelles in Oriented Solid-State NMR: Altered Structural Information in Studies of Antimicrobial Peptides.
Long-Term-Stable Ether-Lipid vs Conventional Ester-Lipid Bicelles in Oriented Solid-State NMR: Altered Structural Information in Studies of Antimicrobial Peptides.
Long-Term-Stable Ether-Lipid vs Conventional Ester-Lipid Bicelles in Oriented Solid-State NMR: Altered Structural Information in Studies of Antimicrobial Peptides.
J Phys Chem B. 2011 Feb 10;
Authors: Bertelsen K, Vad B, Nielsen EH, Hansen SK, Skrydstrup T, Otzen DE, Vosegaard T, Nielsen NC
Recently, ether lipids have been introduced as long-term stable alternatives to the more natural,...
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[NMR paper] Solid-state NMR spectroscopic studies of an integral membrane protein inserted into a
Solid-state NMR spectroscopic studies of an integral membrane protein inserted into aligned phospholipid bilayer nanotube arrays.
Related Articles Solid-state NMR spectroscopic studies of an integral membrane protein inserted into aligned phospholipid bilayer nanotube arrays.
J Am Chem Soc. 2004 Aug 11;126(31):9504-5
Authors: Lorigan GA, Dave PC, Tiburu EK, Damodaran K, Abu-Baker S, Karp ES, Gibbons WJ, Minto RE
This communication demonstrates for the first time that solid-state NMR spectroscopic studies can be used to investigate aligned...
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[NMR paper] Phosphorylated amino acids: model compounds for solid-state 31P NMR spectroscopic stu
Phosphorylated amino acids: model compounds for solid-state 31P NMR spectroscopic studies of proteins.
Related Articles Phosphorylated amino acids: model compounds for solid-state 31P NMR spectroscopic studies of proteins.
Magn Reson Chem. 2004 Apr;42(4):369-72
Authors: Iuga A, Brunner E
Solid-state 31P NMR spectroscopy was applied to measure the isotropic chemical shifts, chemical shift anisotropies and asymmetry parameters of three phosphorylated amino acids, O-phospho-L-serine, O-phospho-L-threonine and O-phospho-L-tyrosine. The...
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[NMR paper] Investigation of the dynamics of an elastin-mimetic polypeptide using solid-state NMR
Investigation of the dynamics of an elastin-mimetic polypeptide using solid-state NMR.
Related Articles Investigation of the dynamics of an elastin-mimetic polypeptide using solid-state NMR.
Magn Reson Chem. 2004 Feb;42(2):267-75
Authors: Yao XL, Conticello VP, Hong M
Elastin is the main structural protein that provides elasticity to various tissues and organs in vertebrates. Molecular motions are believed to play a significant role in its elasticity. We have used solid-state NMR spectroscopy to characterize the dynamics of an elastin-mimetic...
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[NMR paper] Solid-state NMR investigation of the buried X-proline peptide bonds of bacteriorhodop
Solid-state NMR investigation of the buried X-proline peptide bonds of bacteriorhodopsin.
Related Articles Solid-state NMR investigation of the buried X-proline peptide bonds of bacteriorhodopsin.
Biochemistry. 2003 Apr 1;42(12):3586-93
Authors: Lansing JC, Hu JG, Belenky M, Griffin RG, Herzfeld J
The role of proline residues in the photocycle of bacteriorhodopsin (bR) is addressed using solid-state NMR. (13)C and (15)N chemical shifts from X-Pro peptide bonds in bR are assigned from REDOR difference spectra of pairwise labeled samples, and...
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[NMR paper] Solid-state NMR investigation of the dynamics of the soluble and membrane-bound colic
Solid-state NMR investigation of the dynamics of the soluble and membrane-bound colicin Ia channel-forming domain.
Related Articles Solid-state NMR investigation of the dynamics of the soluble and membrane-bound colicin Ia channel-forming domain.
Biochemistry. 2001 Jun 26;40(25):7662-74
Authors: Huster D, Xiao L, Hong M
Solid-state NMR spectroscopy was employed to study the molecular dynamics of the colicin Ia channel domain in the soluble and membrane-bound states. In the soluble state, the protein executes small-amplitude librations (with...
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NMR-Spectroscopic and Solid-State Investigations of Cometal-Free Asymmetric Conjugate
NMR-Spectroscopic and Solid-State Investigations of Cometal-Free Asymmetric Conjugate Addition: A Dinuclear Paracyclophaneimine Zinc Methyl Complex
S. Ay et al
http://pubs.acs.org//appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja1032502/aop/images/medium/ja-2010-032502_0003.gifJournal of the American Chemical Society, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable).
Source: Journal of the American Chemical Society
A 2H solid-state NMR spectroscopic investigation of biomimetic bicelles containing ch
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