The higher-order structure (HOS) of protein therapeutics has been confirmed as a critical quality parameter. In this study, we compared 2D ¹H-^(13)C ALSOFAST-HMQC NMR spectra with immunochemical ELISA-based analysis to evaluate their sensitivity in assessing the HOS of a potent human monoclonal antibody (mAb) for the treatment of coronavirus disease 2019 (COVID-19). The study confirmed that the methyl region of the 2D ¹H-^(13)C NMR spectrum is sensitive to changes in the secondary and tertiary...
[NMR paper] Use of the 2D (1)H-(13)C HSQC NMR Methyl Region to Evaluate the Higher Order Structural Integrity of Biopharmaceuticals
Use of the 2D (1)H-(13)C HSQC NMR Methyl Region to Evaluate the Higher Order Structural Integrity of Biopharmaceuticals
The higher-order structure (HOS) of protein therapeutics is directly related to the function and represents a critical quality attribute. Currently, the HOS of protein therapeutics is characterized by methods with low to medium structural resolution, such as Fourier transform infrared (FTIR), circular dichroism (CD), intrinsic fluorescence spectroscopy (FLD), and differential scanning calorimetry (DSC). High-resolution nuclear magnetic resonance (NMR) methods have now...
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06-02-2021 05:10 PM
[NMR paper] Assessment of the Higher-Order Structure of Formulated Monoclonal Antibody Therapeutics by 2D Methyl Correlated NMR and Principal Component Analysis.
Assessment of the Higher-Order Structure of Formulated Monoclonal Antibody Therapeutics by 2D Methyl Correlated NMR and Principal Component Analysis.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-7388-69-wiley-full-text.png Assessment of the Higher-Order Structure of Formulated Monoclonal Antibody Therapeutics by 2D Methyl Correlated NMR and Principal Component Analysis.
Curr Protoc Protein Sci. 2020 Jun;100(1):e105
Authors: Arbogast LW, Delaglio F, Brinson RG, Marino JP
Abstract
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05-16-2020 02:10 AM
[NMR paper] Comparative Analysis of 1D PROFILE and 2D 1H,13C Methyl NMR Methods for Characterization of the Higher Order Structure of IgG1 Monoclonal Antibodies.
Comparative Analysis of 1D PROFILE and 2D 1H,13C Methyl NMR Methods for Characterization of the Higher Order Structure of IgG1 Monoclonal Antibodies.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Comparative Analysis of 1D PROFILE and 2D 1H,13C Methyl NMR Methods for Characterization of the Higher Order Structure of IgG1 Monoclonal Antibodies.
Anal Chem. 2020 Apr 08;:
Authors: Elliott KW, Ghasriani H, Wikstrom M, Giddens JP, Aubin Y, Delaglio F, Marino JP, Arbogast L
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04-09-2020 07:27 PM
Quality assessment of biologics: higher order structure analysis using NMR - European Pharmaceutical Review
Quality assessment of biologics: higher order structure analysis using NMR European Pharmaceutical ReviewThe webinar will describe how 2D NMR can offer protein biochemists unprecedented analytical precision. By assigning just one spectrum, users can obtain a ...
Quality assessment of biologics: higher order structure analysis using NMR - European Pharmaceutical Review
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01-07-2019 05:49 AM
[NMR paper] Structural insights into the interaction of a monoclonal antibody and Nodal peptides by STD-NMR spectroscopy.
Structural insights into the interaction of a monoclonal antibody and Nodal peptides by STD-NMR spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Structural insights into the interaction of a monoclonal antibody and Nodal peptides by STD-NMR spectroscopy.
Bioorg Med Chem. 2017 Dec 15;25(24):6589-6596
Authors: Calvanese L, Focą A, Sandomenico A, Focą G, Caporale A, Doti N, Iaccarino E, Leonardi A, D'Auria G, Ruvo M, Falcigno L
Abstract
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12-22-2017 07:55 PM
[NMR paper] Effect of Polysorbate 20 and Polysorbate 80 on the Higher Order Structure of a Monoclonal Antibody and its Fab and Fc Fragments Probed Using 2D NMR.
Effect of Polysorbate 20 and Polysorbate 80 on the Higher Order Structure of a Monoclonal Antibody and its Fab and Fc Fragments Probed Using 2D NMR.
Effect of Polysorbate 20 and Polysorbate 80 on the Higher Order Structure of a Monoclonal Antibody and its Fab and Fc Fragments Probed Using 2D NMR.
J Pharm Sci. 2017 Aug 23;:
Authors: Singh SM, Bandi S, Jones DNM, Mallela KMG
Abstract
We examined how polysorbate 20 (PS20; Tween 20) and polysorbate 80 (PS80; Tween 80) affect the higher order structure of a monoclonal antibody (mAb)...
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08-28-2017 10:59 AM
[NMR paper] Simple NMR methods for evaluating higher order structures of monoclonal antibody therapeutics with quinary structure.
Simple NMR methods for evaluating higher order structures of monoclonal antibody therapeutics with quinary structure.
Related Articles Simple NMR methods for evaluating higher order structures of monoclonal antibody therapeutics with quinary structure.
J Pharm Biomed Anal. 2016 Jun 7;128:398-407
Authors: Chen K, Long DS, Lute SC, Levy MJ, Brorson KA, Keire DA
Abstract
Monoclonal antibody (mAb) drugs constitute the largest class of protein therapeutics currently on the market. Correctly folded protein higher order structure...
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06-28-2016 06:25 PM
[NMR paper] Mapping monoclonal antibody structure by 2D 13C NMR at natural abundance.
Mapping monoclonal antibody structure by 2D 13C NMR at natural abundance.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Mapping monoclonal antibody structure by 2D 13C NMR at natural abundance.
Anal Chem. 2015 Apr 7;87(7):3556-61
Authors: Arbogast LW, Brinson RG, Marino JP
Abstract
Monoclonal antibodies (mAbs) represent an important and rapidly growing class of biotherapeutics. Correct folding of a mAb is critical for drug efficacy, while misfolding...
2D NMR Analysis as a Sensitive Tool for Evaluating the Higher-Order Structural Integrity of Monoclonal Antibody against COVID-19
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