A 250 GHz Gyrotron with a 3 GHz Tuning Bandwidth for Dynamic Nuclear Polarization

		BioNMR > Educational resources > Journal club
A 250 GHz Gyrotron with a 3 GHz Tuning Bandwidth for Dynamic Nuclear Polarization

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

03-30-2012, 10:45 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,776

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

A 250 GHz Gyrotron with a 3 GHz Tuning Bandwidth for Dynamic Nuclear Polarization

A 250 GHz Gyrotron with a 3 GHz Tuning Bandwidth for Dynamic Nuclear Polarization

Publication year: 2012
Source:Journal of Magnetic Resonance

Alexander B. Barnes, Emilio A. Nanni, Judith Herzfeld, Robert G. Griffin, Richard J. Temkin

We describe the design and implementation of a novel tunable 250 GHz gyrotron oscillator with >10 W output power over most of a 3 GHz band and >35 W peak power. The tuning bandwidth and power are sufficient to generate a >1 MHz nutation frequency across the entire nitroxide EPR lineshape for cross effect DNP, as well as to excite solid effect transitions utilizing other radicals, without the need for sweeping the NMR magnetic field. Substantially improved tunability is achieved by implementing a long (23 mm) interaction cavity that can excite higher order axial modes by changing either the magnetic field of the gyrotron or the cathode potential. This interaction cavity excites the rotating TE5,2,q mode, and an internal mode converter outputs a high-quality microwave beam with >94% Gaussian content. The gyrotron was integrated into a DNP spectrometer, resulting in a measured DNP enhancement of 54 on the membrane protein bacteriorhodopsin.
Graphical Abstract

Graphical abstract Highlights

? 3 GHz tunable bandwidth for DNP with > 10 watts of output power. ? Larger enhancements for bacteriohodopsin. ? TE5,2,q operating mode.

Source: Journal of Magnetic Resonance

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Dynamic nuclear polarization at high magnetic fields in liquids Dynamic nuclear polarization at high magnetic fields in liquids Publication year: 2011 Source:Progress in Nuclear Magnetic Resonance Spectroscopy</br> C. Griesinger, M. Bennati, H.M. Vieth, C. Luchinat, G. Parigi, P. Höfer, F. Engelke, S.J. Glaser, V. Denysenkov, T.F. Prisner</br> Graphical Abstract http://ars.sciencedirect.com/content/image/1-s2.0-S0079656511000689-fx1.jpg Graphical abstract Highlights	nmrlearner	Journal club	0	03-09-2012 09:16 AM
Neurotoxin II Bound to Acetylcholine Receptors in Native Membranes Studied by Dynamic Nuclear Polarization NMR Neurotoxin II Bound to Acetylcholine Receptors in Native Membranes Studied by Dynamic Nuclear Polarization NMR Arne H. Linden, Sascha Lange, W. Trent Franks, U?mit Akbey, Edgar Specker, Barth-Jan van Rossum and Hartmut Oschkinat http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja206999c/aop/images/medium/ja-2011-06999c_0003.gif Journal of the American Chemical Society DOI: 10.1021/ja206999c http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/4d1LYPHtCFw	nmrlearner	Journal club	0	11-11-2011 08:26 AM
Dynamic Nuclear Polarization at High Magnetic Fields in Liquids Dynamic Nuclear Polarization at High Magnetic Fields in Liquids Publication year: 2011 Source: Progress in Nuclear Magnetic Resonance Spectroscopy, Available online 4 November 2011</br> C.Griesinger, M.Bennati, H.M.Vieth, C.Luchinat, G.*Parigi, ...</br> Highlights ? High field Dynamic Nuclear Polarization spectrometers for liquid samples have been constructed, working at 7, 9.2 and 14 T, respectively. ? The field dependence of the Overhauser DNP efficiency has been measured experimentally for the first time up to a field of 9.2 T and compared with experimental results from...	nmrlearner	Journal club	0	11-05-2011 09:21 AM
Solution NMR of Polypeptides Hyperpolarized by Dynamic Nuclear Polarization. Solution NMR of Polypeptides Hyperpolarized by Dynamic Nuclear Polarization. Solution NMR of Polypeptides Hyperpolarized by Dynamic Nuclear Polarization. Anal Chem. 2011 Jun 7; Authors: Ragavan M, Chen HY, Sekar G, Hilty C Hyperpolarization of nuclear spins through techniques such as Dynamic Nuclear Polarization (DNP) can greatly increase the signal to noise ratio in NMR measurements, thus eliminating the need for signal averaging. This enables the study of many dynamic processes which would otherwise not be amenable to study by NMR spectroscopy....	nmrlearner	Journal club	0	06-10-2011 11:52 AM
Quantitative dynamic nuclear polarization-NMR on blood plasma for assays of drug meta Quantitative dynamic nuclear polarization-NMR on blood plasma for assays of drug metabolism. Related Articles Quantitative dynamic nuclear polarization-NMR on blood plasma for assays of drug metabolism. NMR Biomed. 2010 Sep 22; Authors: Lerche MH, Meier S, Jensen PR, Hustvedt SO, Karlsson M, Duus JO, ArdenkjÃ¦r-Larsen JH Analytical platforms for the fast detection, identification and quantification of circulating drugs with a narrow therapeutic range are vital in clinical pharmacology. As a result of low drug concentrations, analytical tools...	nmrlearner	Journal club	0	09-24-2010 04:57 PM
Surface Enhanced NMR Spectroscopy by Dynamic Nuclear Polarization Surface Enhanced NMR Spectroscopy by Dynamic Nuclear Polarization Anne Lesage, Moreno Lelli, David Gajan, Marc A. Caporini, Veronika Vitzthum, Pascal Mie?ville, Johan Alauzun, Arthur Roussey, Chloe? Thieuleux, Ahmad Medhi, Geoffrey Bodenhausen, Christophe Cope?ret and Lyndon Emsley http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja104771z/aop/images/medium/ja-2010-04771z_0004.gif Journal of the American Chemical Society DOI: 10.1021/ja104771z http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA...	nmrlearner	Journal club	0	09-11-2010 01:25 AM
Dynamic nuclear polarization-enhanced solid-state NMR spectroscopy of GNNQQNY nanocry Dynamic nuclear polarization-enhanced solid-state NMR spectroscopy of GNNQQNY nanocrystals and amyloid fibrils. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.rsc.org-images-entities-char_z_RSClogo.gif Related Articles Dynamic nuclear polarization-enhanced solid-state NMR spectroscopy of GNNQQNY nanocrystals and amyloid fibrils. Phys Chem Chem Phys. 2010 Jun 14;12(22):5911-9 Authors: Debelouchina GT, Bayro MJ, van der Wel PC, Caporini MA, Barnes AB, Rosay M, Maas WE, Griffin RG Dynamic nuclear polarization (DNP) utilizes the...	nmrlearner	Journal club	0	08-26-2010 04:41 PM
Dynamic nuclear polarization experiments at 14.1 T for solid-state NMR. Dynamic nuclear polarization experiments at 14.1 T for solid-state NMR. Related Articles Dynamic nuclear polarization experiments at 14.1 T for solid-state NMR. Phys Chem Chem Phys. 2010 Jun 14;12(22):5799-803 Authors: Matsuki Y, Takahashi H, Ueda K, Idehara T, Ogawa I, Toda M, Akutsu H, Fujiwara T Instrumentation for high-field dynamic nuclear polarization (DNP) at 14.1 T was developed to enhance the nuclear polarization for NMR of solids. The gyrotron generated 394.5 GHz submillimeter (sub-mm) wave with a power of 40 W in the second harmonic...	nmrlearner	Journal club	0	08-26-2010 04:41 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off