Publication year: 2010 Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 3 September 2010
S., Jayanthi , N., Sinha , K.V., Ramanathan
Separated Local Field (SLF) spectroscopy is a powerful tool for the determination of structure and dynamics of oriented systems such as membrane proteins oriented in lipid bilayers and liquid crystals. Of many SLF techniques available, Polarization Inversion Spin Exchange at Magic Angle (PISEMA) has found wide application due to its many favorable characteristics. However the pulse sequence suffers from its sensitivity to proton resonance frequency offset. Recently we have proposed a new sequence named 24-SEMA (J. Chem. Phys. 132, 134301, 2010) that overcomes this problem of PISEMA. The present work demonstrates the advantage of 24-SEMA as a highly sensitive SLF...
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We propose a ligand screening method, called TINS (target immobilized NMR screening), which reduces the amount of target required for the fragment-based approach to drug discovery. Binding is detected by...
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Backbone Amide Dynamics Studies of Apo-L75F-TrpR, a Temperature-Sensitive Mutant of t
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Backbone amide dynamics studies of apo-L75F-TrpR, a temperature sensitive mutant of t
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Biochemistry. 2010 Aug 18;
Authors: Goel A, Tripet BP, Tyler RC, Nebert LD, Copie V
...
24-SEMA as a Sensitive and Offset Compensated SLF Sequence
Linear Mode
