NMR paper 1H resonance assignments, secondary structure and general topology of single-chain mo

		BioNMR > Educational resources > Journal club
[NMR paper] 1H resonance assignments, secondary structure and general topology of single-chain mo

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-21-2010, 11:45 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

1H resonance assignments, secondary structure and general topology of single-chain mo

1H resonance assignments, secondary structure and general topology of single-chain monellin in solution as determined by 1H 2D-NMR.

Related Articles 1H resonance assignments, secondary structure and general topology of single-chain monellin in solution as determined by 1H 2D-NMR.

J Biomol NMR. 1992 Nov;2(6):557-72

Authors: Tomic MT, Somoza JR, Wemmer DE, Park YW, Cho JM, Kim SH

We determined the resonance assignments, secondary structure and general topology of the 11-kDa sweet protein single-chain monellin (SCM), using two-dimensional proton nuclear magnetic resonance spectroscopy (2D-NMR). SCM is a genetically engineered protein whose design is based on the crystal structure of natural, two-chain monellin (Kim et al., 1989). Analysis of the NMR spectra shows that the secondary structure of SCM consists of a five-strand anti-parallel beta-sheet and a 15-residue alpha-helix. Tertiary NOE constraints place the alpha-helix on the hydrophobic side of the beta-sheet, and indicate that the sheet is partially wrapped around the helix. The general structural features determined for SCM are similar to those of native monellin (Ogata et al., 1987). Some differences between the SCM structure in solution and the crystal structure of monellin are discussed.

PMID: 1490107 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Multinuclear NMR resonance assignments and the secondary structure of Escherichia col Multinuclear NMR resonance assignments and the secondary structure of Escherichia coli thioesterase/protease I: a member of a new subclass of lipolytic enzymes. Related Articles Multinuclear NMR resonance assignments and the secondary structure of Escherichia coli thioesterase/protease I: a member of a new subclass of lipolytic enzymes. J Biomol NMR. 1998 May;11(4):363-80 Authors: Lin TH, Chen C, Huang RF, Lee YL, Shaw JF, Huang TH Escherichia coli thioesterase/protease I is a 183 amino acid protein with a molecular mass of 20,500. This...	nmrlearner	Journal club	0	11-17-2010 11:06 PM
[NMR paper] 1H, 15N and 13C NMR assignments, secondary structure and overall topology of the Esch 1H, 15N and 13C NMR assignments, secondary structure and overall topology of the Escherichia coli GlgS protein. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles 1H, 15N and 13C NMR assignments, secondary structure and overall topology of the Escherichia coli GlgS protein. Eur J Biochem. 1997 Jun 1;246(2):301-10 Authors: Beglova N, Fischer D, Hengge-Aronis R, Gehring K GlgS is a 7892-Da protein which is involved in glycogen...	nmrlearner	Journal club	0	08-22-2010 03:31 PM
[NMR paper] 1H, 15N and 13C NMR assignments, secondary structure and overall topology of the Esch 1H, 15N and 13C NMR assignments, secondary structure and overall topology of the Escherichia coli GlgS protein. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles 1H, 15N and 13C NMR assignments, secondary structure and overall topology of the Escherichia coli GlgS protein. Eur J Biochem. 1997 Jun 1;246(2):301-10 Authors: Beglova N, Fischer D, Hengge-Aronis R, Gehring K GlgS is a 7892-Da protein which is involved in glycogen...	nmrlearner	Journal club	0	08-22-2010 03:03 PM
[NMR paper] 1H, 13C, and 15N NMR resonance assignments, secondary structure, and backbone topolog 1H, 13C, and 15N NMR resonance assignments, secondary structure, and backbone topology of a variant of human interleukin-3. Related Articles 1H, 13C, and 15N NMR resonance assignments, secondary structure, and backbone topology of a variant of human interleukin-3. Biochemistry. 1995 May 16;34(19):6540-51 Authors: Feng Y, Klein BK, Vu L, Aykent S, McWherter CA Interleukin-3 (IL-3) is a cytokine which stimulates the proliferation and differentiation of hematopoietic progenitors into multiple cell lineages. The 1H, 15N, and 13C NMR resonances of...	nmrlearner	Journal club	0	08-22-2010 03:41 AM
[NMR paper] Secondary structure and topology of interleukin-1 receptor antagonist protein determi Secondary structure and topology of interleukin-1 receptor antagonist protein determined by heteronuclear three-dimensional NMR spectroscopy. Related Articles Secondary structure and topology of interleukin-1 receptor antagonist protein determined by heteronuclear three-dimensional NMR spectroscopy. Biochemistry. 1992 Jun 16;31(23):5237-45 Authors: Stockman BJ, Scahill TA, Roy M, Ulrich EL, Strakalaitis NA, Brunner DP, Yem AW, Deibel MR Interleukin-1 (IL-1) proteins, such as IL-1 beta, play a key role in immune and inflammatory responses....	nmrlearner	Journal club	0	08-21-2010 11:41 PM
[NMR paper] Secondary structure and side-chain 1H and 13C resonance assignments of calmodulin in Secondary structure and side-chain 1H and 13C resonance assignments of calmodulin in solution by heteronuclear multidimensional NMR spectroscopy. Related Articles Secondary structure and side-chain 1H and 13C resonance assignments of calmodulin in solution by heteronuclear multidimensional NMR spectroscopy. Biochemistry. 1991 Sep 24;30(38):9216-28 Authors: Ikura M, Spera S, Barbato G, Kay LE, Krinks M, Bax A Heteronuclear 2D and 3D NMR experiments were carried out on recombinant Drosophila calmodulin (CaM), a protein of 148 residues and with...	nmrlearner	Journal club	0	08-21-2010 11:12 PM
[NMR paper] Secondary structure and side-chain 1H and 13C resonance assignments of calmodulin in Secondary structure and side-chain 1H and 13C resonance assignments of calmodulin in solution by heteronuclear multidimensional NMR spectroscopy. Related Articles Secondary structure and side-chain 1H and 13C resonance assignments of calmodulin in solution by heteronuclear multidimensional NMR spectroscopy. Biochemistry. 1991 Sep 24;30(38):9216-28 Authors: Ikura M, Spera S, Barbato G, Kay LE, Krinks M, Bax A Heteronuclear 2D and 3D NMR experiments were carried out on recombinant Drosophila calmodulin (CaM), a protein of 148 residues and with...	nmrlearner	Journal club	0	08-21-2010 11:12 PM
[NMR paper] Determination of the secondary structure and molecular topology of interleukin-1 beta Determination of the secondary structure and molecular topology of interleukin-1 beta by use of two- and three-dimensional heteronuclear 15N-1H NMR spectroscopy. Related Articles Determination of the secondary structure and molecular topology of interleukin-1 beta by use of two- and three-dimensional heteronuclear 15N-1H NMR spectroscopy. Biochemistry. 1990 May 15;29(19):4668-82 Authors: Driscoll PC, Gronenborn AM, Wingfield PT, Clore GM A study of the regular secondary structure elements of recombinant human interleukin-1 beta has been...	nmrlearner	Journal club	0	08-21-2010 10:48 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off