NMR paper 1H-NMR studies on water structures in the rat brain tissues with cerebral tumour or i

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[NMR paper] 1H-NMR studies on water structures in the rat brain tissues with cerebral tumour or i

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NMR processing:

MDD

NMR assignment:

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Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

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GeNMR

I-TASSER

Refinement:

Amber

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Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

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TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

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NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

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SAVES2 or SAVES4

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Methyl S2

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Shifty

Camcoil

Poulsen_rc_CS

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08-21-2010, 10:48 PM

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1H-NMR studies on water structures in the rat brain tissues with cerebral tumour or i

1H-NMR studies on water structures in the rat brain tissues with cerebral tumour or ischaemia.

Related Articles 1H-NMR studies on water structures in the rat brain tissues with cerebral tumour or ischaemia.

Acta Neurochir Suppl (Wien). 1990;51:125-7

Authors: Iwama T, Andoh T, Sakai N, Yamada H, Era S, Kuwata K, Sogami M, Watari H

Spin-lattice relaxation times (T1) of water protons and cross-relaxation times (T(IS)) between irradiated protein protons and observed water protons were measured to study water structure in various rat brain tissue. A tumour-bearing rat model and an incomplete forebrain ischaemia model of stroke prone spontaneously hypertensive rats (SHRSP) were used for the experiments. T(IS) and T1 were measured by the inversion recovery FT method with and without f2-irradiation at 7.13 p.p.m. and gamma H2/2 pi approximately 69 Hz, respectively, using a 360 MHz FT-NMR spectrometer. All of experiments on T1 and T(IS) yielded a single kind of component. In spite of small changes in T1 and water content, T(IS) values in peritumoural oedematous and ischaemic brain tissue were significantly shorter than those of normal brain and far shorter than those of brain tumour, indicating a large amount of hydration of marco-molecules in the oedematous and/or ischaemic rat brain tissue. T(IS) might be sensitive parameter for studying the water structure in various brain tissue.

PMID: 2089876 [PubMed - indexed for MEDLINE]

Source: PubMed

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