NMR paper 1H-NMR studies on partially and fully reduced 2(4Fe-4S) ferredoxin from Clostridium p

		BioNMR > Educational resources > Journal club
[NMR paper] 1H-NMR studies on partially and fully reduced 2(4Fe-4S) ferredoxin from Clostridium p

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-21-2010, 11:41 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

1H-NMR studies on partially and fully reduced 2(4Fe-4S) ferredoxin from Clostridium p

1H-NMR studies on partially and fully reduced 2(4Fe-4S) ferredoxin from Clostridium pasteurianum.

Related Articles 1H-NMR studies on partially and fully reduced 2(4Fe-4S) ferredoxin from Clostridium pasteurianum.

Eur J Biochem. 1992 Mar 1;204(2):831-9

Authors: Bertini I, Briganti F, Luchinat C, Messori L, Monnanni R, Scozzafava A, Vallini G

The ferredoxin from Clostridium pasteurianum, containing two Fe4S4 clusters, has been investigated through 1H-NMR spectroscopy in the reduced and partially oxidized states. The 1H-NMR spectrum of fully reduced ferredoxin, obtained by addition of stoichiometric amounts of dithionite, has been characterized. One- and two-dimensional NMR saturation transfer experiments on partially reduced samples have allowed the isotropically shifted signals of the reduced form to be correlated to those of the oxidized form, for which the complete assignment of the beta-CH2 cysteinyl residues is available. In addition, observation of the 1H-NMR signals of the intermediate species with characteristic chemical shift values for each cluster allowed us to assign all the Cys beta-CH2 signals to cluster I or cluster II and to calculate the difference in redox potential between them. Starting from these results, reanalysis of the 1H-NMR features of the two clusters in the oxidized form showed that they are strikingly similar, supporting the idea of a high degree of internal symmetry between them, in agreement with crystallographic results on an homologous ferredoxin. On the other hand, the 1H-NMR properties of the two clusters in the reduced form deviate considerably from each other, suggesting that reduction of the clusters brings about different structural changes and loss of internal symmetry. A theoretical approach is reported to account for the isotropic shifts and the temperature dependence of the NMR signals of the reduced protein.

PMID: 1541295 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Radio frequency assisted homonuclear recoupling - A Floquet description of homonuclear recoupling via surrounding heteronuclei in fully protonated to fully deuterated systems Radio frequency assisted homonuclear recoupling - A Floquet description of homonuclear recoupling via surrounding heteronuclei in fully protonated to fully deuterated systems Publication year: 2011 Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 18 January 2011</br> Michal, Leskes , Ümit, Akbey , Hartmut, Oschkinat , Barth-Jan, van Rossum , Shimon, Vega</br> We present a Floquet theory approach for the analysis of homonuclear recoupling assisted by radio frequency (RF) irradiation of surrounding heteronuclear spins. This description covers a...	nmrlearner	Journal club	0	01-19-2011 03:04 PM
[NMR paper] Synechocystis ferredoxin/ferredoxin-NADP(+)-reductase/NADP+ complex: Structural model obtained by NMR-restrained docking. Synechocystis ferredoxin/ferredoxin-NADP(+)-reductase/NADP+ complex: Structural model obtained by NMR-restrained docking. Related Articles Synechocystis ferredoxin/ferredoxin-NADP(+)-reductase/NADP+ complex: Structural model obtained by NMR-restrained docking. FEBS Lett. 2005 Aug 29;579(21):4585-90 Authors: Palma PN, Lagoutte B, Krippahl L, Moura JJ, Guerlesquin F Ferredoxin (Fd) and ferredoxin-NADP(+)-reductase (FNR) are two terminal physiological partners of the photosynthetic electron transport chain. Based on a nuclear magnetic resonance...	nmrlearner	Journal club	0	12-01-2010 06:56 PM
[NMR paper] 13C-, 15N- and 31P-NMR studies of oxidized and reduced low molecular mass thioredoxin 13C-, 15N- and 31P-NMR studies of oxidized and reduced low molecular mass thioredoxin reductase and some mutant proteins. Related Articles 13C-, 15N- and 31P-NMR studies of oxidized and reduced low molecular mass thioredoxin reductase and some mutant proteins. Eur J Biochem. 2004 Apr;271(8):1437-52 Authors: Eisenreich W, Kemter K, Bacher A, Mulrooney SB, Williams CH, Müller F Thioredoxin reductase (TrxR) from Escherichia coli, the mutant proteins E159Y and C138S, and the mutant protein C138S treated with phenylmercuric acetate were...	nmrlearner	Journal club	0	11-24-2010 09:51 PM
[NMR paper] A proton-NMR investigation of the fully reduced cytochrome c7 from Desulfuromonas ace A proton-NMR investigation of the fully reduced cytochrome c7 from Desulfuromonas acetoxidans. Comparison between the reduced and the oxidized forms. Related Articles A proton-NMR investigation of the fully reduced cytochrome c7 from Desulfuromonas acetoxidans. Comparison between the reduced and the oxidized forms. Eur J Biochem. 1999 Dec;266(2):634-43 Authors: Assfalg M, Banci L, Bertini I, Bruschi M, Giudici-Orticoni MT, Turano P The solution structure via 1H NMR of the fully reduced form of cytochrome c7 has been obtained. The protein...	nmrlearner	Journal club	0	11-18-2010 08:31 PM
[NMR paper] NMR spectroscopic studies of the hydrogenosomal [2Fe-2S] ferredoxin from Trichomonas NMR spectroscopic studies of the hydrogenosomal ferredoxin from Trichomonas vaginalis: hyperfine-shifted 1H resonances. Related Articles NMR spectroscopic studies of the hydrogenosomal ferredoxin from Trichomonas vaginalis: hyperfine-shifted 1H resonances. J Inorg Biochem. 1998 Dec;72(3-4):127-31 Authors: Liu HY, Germanas JP The hyperfine-shifted 1H NMR resonances of oxidized and reduced Trichomonas vaginalis ferredoxin, a functionally unique ferredoxin, have been studied. The oxidized protein spectrum displayed a pattern of six broad...	nmrlearner	Journal club	0	11-17-2010 11:15 PM
[NMR tweet] Dynamic studies of phloem and xylem flow in fully differentiated plants by fast nucle Dynamic studies of phloem and xylem flow in fully differentiated plants by fast nuclear-magnetic-resonance microima... http://ping.fm/nfA2k Published by stevenbird64 (Steven Bird) on 2010-10-03T16:25:36Z Source: Twitter	nmrlearner	Twitter NMR	0	10-03-2010 04:58 PM
[NMR paper] 1H NMR studies on the oxidized ferredoxin from Clostridium pasteurianum. 1H NMR studies on the oxidized ferredoxin from Clostridium pasteurianum. Related Articles 1H NMR studies on the oxidized ferredoxin from Clostridium pasteurianum. Biochem Int. 1992 Mar;26(4):577-85 Authors: Ganadu ML, Bonomi F, Pagani S, Boelens R The 8Fe-8S ferredoxin from Clostridium pasteurianum was investigated by 1D and 2D 1H NMR. Spectra of a well-structured, full native preparation of the oxidized protein in 1 M NaCl at pH 8.0 are presented. Assignments of non-isotropically shifted resonances in the diamagnetic region of the spectrum,...	nmrlearner	Journal club	0	08-21-2010 11:41 PM
[NMR paper] 13C-NMR of Clostridium pasteurianum ferredoxin after reductive methylation of the ami 13C-NMR of Clostridium pasteurianum ferredoxin after reductive methylation of the amines using formaldehyde. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles 13C-NMR of Clostridium pasteurianum ferredoxin after reductive methylation of the amines using formaldehyde. Biochim Biophys Acta. 1990 Apr 19;1038(2):146-51 Authors: Gluck M, Sweeney WV Clostridium pasteurianum 2(4Fe-4S) ferredoxin has been reductively methylated using formaldehyde and sodium cyanoborohydride....	nmrlearner	Journal club	0	08-21-2010 10:48 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off