NMR paper 1H NMR studies on the oxidized ferredoxin from Clostridium pasteurianum.

		BioNMR > Educational resources > Journal club
[NMR paper] 1H NMR studies on the oxidized ferredoxin from Clostridium pasteurianum.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-21-2010, 11:41 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,734

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

1H NMR studies on the oxidized ferredoxin from Clostridium pasteurianum.

1H NMR studies on the oxidized ferredoxin from Clostridium pasteurianum.

Related Articles 1H NMR studies on the oxidized ferredoxin from Clostridium pasteurianum.

Biochem Int. 1992 Mar;26(4):577-85

Authors: Ganadu ML, Bonomi F, Pagani S, Boelens R

The 8Fe-8S ferredoxin from Clostridium pasteurianum was investigated by 1D and 2D 1H NMR. Spectra of a well-structured, full native preparation of the oxidized protein in 1 M NaCl at pH 8.0 are presented. Assignments of non-isotropically shifted resonances in the diamagnetic region of the spectrum, namely those of the unique aromatic residues F30 and Y2, are presented for the first time.

PMID: 1610368 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Synechocystis ferredoxin/ferredoxin-NADP(+)-reductase/NADP+ complex: Structural model obtained by NMR-restrained docking. Synechocystis ferredoxin/ferredoxin-NADP(+)-reductase/NADP+ complex: Structural model obtained by NMR-restrained docking. Related Articles Synechocystis ferredoxin/ferredoxin-NADP(+)-reductase/NADP+ complex: Structural model obtained by NMR-restrained docking. FEBS Lett. 2005 Aug 29;579(21):4585-90 Authors: Palma PN, Lagoutte B, Krippahl L, Moura JJ, Guerlesquin F Ferredoxin (Fd) and ferredoxin-NADP(+)-reductase (FNR) are two terminal physiological partners of the photosynthetic electron transport chain. Based on a nuclear magnetic resonance...	nmrlearner	Journal club	0	12-01-2010 06:56 PM
[NMR paper] 13C-, 15N- and 31P-NMR studies of oxidized and reduced low molecular mass thioredoxin 13C-, 15N- and 31P-NMR studies of oxidized and reduced low molecular mass thioredoxin reductase and some mutant proteins. Related Articles 13C-, 15N- and 31P-NMR studies of oxidized and reduced low molecular mass thioredoxin reductase and some mutant proteins. Eur J Biochem. 2004 Apr;271(8):1437-52 Authors: Eisenreich W, Kemter K, Bacher A, Mulrooney SB, Williams CH, Müller F Thioredoxin reductase (TrxR) from Escherichia coli, the mutant proteins E159Y and C138S, and the mutant protein C138S treated with phenylmercuric acetate were...	nmrlearner	Journal club	0	11-24-2010 09:51 PM
[NMR paper] NMR spectroscopic studies of the hydrogenosomal [2Fe-2S] ferredoxin from Trichomonas NMR spectroscopic studies of the hydrogenosomal ferredoxin from Trichomonas vaginalis: hyperfine-shifted 1H resonances. Related Articles NMR spectroscopic studies of the hydrogenosomal ferredoxin from Trichomonas vaginalis: hyperfine-shifted 1H resonances. J Inorg Biochem. 1998 Dec;72(3-4):127-31 Authors: Liu HY, Germanas JP The hyperfine-shifted 1H NMR resonances of oxidized and reduced Trichomonas vaginalis ferredoxin, a functionally unique ferredoxin, have been studied. The oxidized protein spectrum displayed a pattern of six broad...	nmrlearner	Journal club	0	11-17-2010 11:15 PM
[NMR paper] Solution structure of the oxidized Fe7S8 ferredoxin from the thermophilic bacterium B Solution structure of the oxidized Fe7S8 ferredoxin from the thermophilic bacterium Bacillus schlegelii by 1H NMR spectroscopy. Related Articles Solution structure of the oxidized Fe7S8 ferredoxin from the thermophilic bacterium Bacillus schlegelii by 1H NMR spectroscopy. Biochemistry. 1998 Jul 7;37(27):9812-26 Authors: Aono S, Bentrop D, Bertini I, Donaire A, Luchinat C, Niikura Y, Rosato A The solution structure of the paramagnetic seven-iron ferredoxin from Bacillus schlegelii in its oxidized form has been determined by 1H NMR. The protein,...	nmrlearner	Journal club	0	11-17-2010 11:15 PM
[NMR paper] Homonuclear and heteronuclear NMR studies of oxidized Desulfovibrio vulgaris flavodox Homonuclear and heteronuclear NMR studies of oxidized Desulfovibrio vulgaris flavodoxin. Sequential assignments and identification of secondary structure elements. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Homonuclear and heteronuclear NMR studies of oxidized Desulfovibrio vulgaris flavodoxin. Sequential assignments and identification of secondary structure elements. Eur J Biochem. 1993 Apr 1;213(1):167-84 Authors: Knauf MA, LÃ¶hr F,...	nmrlearner	Journal club	0	08-21-2010 11:53 PM
[NMR paper] 1H-NMR studies on partially and fully reduced 2(4Fe-4S) ferredoxin from Clostridium p 1H-NMR studies on partially and fully reduced 2(4Fe-4S) ferredoxin from Clostridium pasteurianum. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles 1H-NMR studies on partially and fully reduced 2(4Fe-4S) ferredoxin from Clostridium pasteurianum. Eur J Biochem. 1992 Mar 1;204(2):831-9 Authors: Bertini I, Briganti F, Luchinat C, Messori L, Monnanni R, Scozzafava A, Vallini G The ferredoxin from Clostridium pasteurianum, containing two...	nmrlearner	Journal club	0	08-21-2010 11:41 PM
[NMR paper] 1H NMR studies of oxidized high-potential iron protein from Chromatium vinosum. Nucle 1H NMR studies of oxidized high-potential iron protein from Chromatium vinosum. Nuclear Overhauser effect measurements. Related Articles 1H NMR studies of oxidized high-potential iron protein from Chromatium vinosum. Nuclear Overhauser effect measurements. Biochemistry. 1990 Jun 12;29(23):5633-7 Authors: Cowan JA, Sola M 1H nuclear Overhauser effect experiments on the isotropically shifted signals of oxidized Chromatium vinosum HiPIP have been used to identify the four beta-CH2 geminal couples of the cysteine ligands. A partial assignment to...	nmrlearner	Journal club	0	08-21-2010 10:48 PM
[NMR paper] 13C-NMR of Clostridium pasteurianum ferredoxin after reductive methylation of the ami 13C-NMR of Clostridium pasteurianum ferredoxin after reductive methylation of the amines using formaldehyde. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles 13C-NMR of Clostridium pasteurianum ferredoxin after reductive methylation of the amines using formaldehyde. Biochim Biophys Acta. 1990 Apr 19;1038(2):146-51 Authors: Gluck M, Sweeney WV Clostridium pasteurianum 2(4Fe-4S) ferredoxin has been reductively methylated using formaldehyde and sodium cyanoborohydride....	nmrlearner	Journal club	0	08-21-2010 10:48 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off