NMR paper 1H NMR studies on the interaction of beta-carboline derivatives with human serum albu

		BioNMR > Educational resources > Journal club
[NMR paper] 1H NMR studies on the interaction of beta-carboline derivatives with human serum albu

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-17-2010, 11:06 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

1H NMR studies on the interaction of beta-carboline derivatives with human serum albu

1H NMR studies on the interaction of beta-carboline derivatives with human serum albumin.

Related Articles 1H NMR studies on the interaction of beta-carboline derivatives with human serum albumin.

J Magn Reson. 1998 Feb;130(2):281-6

Authors: Veglia G, Delfini M, Giudice MR, Gaggelli E, Valensin G

1H NMR studies were performed on two beta-carboline derivatives interacting with human serum albumin. The spin-lattice relaxation rates of the two derivatives, having side chains of different length and polarity, were used to demonstrate a diverse motional behavior in solution together with slightly different relaxation pathways. Single- and double-selective excitation made it possible to evaluate dynamics in the free and protein-bound states. Occurrence of a relatively long hydrophilic chain interacting with the proton-acceptor nitrogen of the beta-carboline moiety was shown to yield lower association constants, slower dissociation rates, and diverse interacting modes with the indole hydrophobic site of the protein.

PMID: 9500891 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR900 blog] The Human Serum Metabolome The Human Serum Metabolome N. Psychogios, D.D. Hau, J. Peng, A.C. Guo, R. Mandal, S. Bouatra, I. Sinelnikov, R. Krishnamurthy, R. Eisner, B. Gautam, N. Young, J. Xia, C. Knox, E. Dong, P. Huang, Z. Hollander, T.L. Pedersen, S.R. Smith, F. Bamforth, R. Greiner, B. McManus, J.W. Newman, T. Goodfriend, D.S. Wishart, "The Human Serum Metabolome," PLoS ONE 6 (2011) e16957. (open access article) http://dx.doi.org/10.1371/journal.pone.0016957 Abstract: Continuing improvements in analytical technology along with an increased interest in performing comprehensive, quantitative metabolic...	nmrlearner	News from NMR blogs	0	02-17-2011 09:44 PM
[NMR paper] Determination of protein-ligand binding affinity by NMR: observations from serum albu Determination of protein-ligand binding affinity by NMR: observations from serum albumin model systems. Related Articles Determination of protein-ligand binding affinity by NMR: observations from serum albumin model systems. Magn Reson Chem. 2005 Jun;43(6):463-70 Authors: Fielding L, Rutherford S, Fletcher D The usefulness of bovine serum albumin (BSA) as a model protein for testing NMR methods for the study of protein-ligand interactions is discussed. Isothermal titration calorimetry established the binding affinity and stoichiometry of the...	nmrlearner	Journal club	0	11-25-2010 08:21 PM
[NMR paper] Structural studies of the putative helix 8 in the human beta(2) adrenergic receptor: Structural studies of the putative helix 8 in the human beta(2) adrenergic receptor: an NMR study. Related Articles Structural studies of the putative helix 8 in the human beta(2) adrenergic receptor: an NMR study. Biochim Biophys Acta. 2004 May 27;1663(1-2):74-81 Authors: Katragadda M, Maciejewski MW, Yeagle PL The recently reported crystal structure of bovine rhodopsin revealed a cytoplasmic helix (helix 8) in addition to the seven transmembrane helices. This domain is roughly perpendicular to the transmembrane bundle in the presence of an...	nmrlearner	Journal club	0	11-24-2010 09:51 PM
[NMR paper] Interaction between beta-Purothionin and dimyristoylphosphatidylglycerol: a (31)P-NMR Interaction between beta-Purothionin and dimyristoylphosphatidylglycerol: a (31)P-NMR and infrared spectroscopic study. Related Articles Interaction between beta-Purothionin and dimyristoylphosphatidylglycerol: a (31)P-NMR and infrared spectroscopic study. Biophys J. 2002 Oct;83(4):2074-83 Authors: Richard JA, Kelly I, Marion D, Pézolet M, Auger M The interaction of beta-purothionin, a small basic and antimicrobial protein from the endosperm of wheat seeds, with multilamellar vesicles of dimyristoylphosphatidylglycerol (DMPG) was investigated...	nmrlearner	Journal club	0	11-24-2010 08:58 PM
[NMR paper] 13C NMR studies of the binding of medium-chain fatty acids to human serum albumin. 13C NMR studies of the binding of medium-chain fatty acids to human serum albumin. Related Articles 13C NMR studies of the binding of medium-chain fatty acids to human serum albumin. J Lipid Res. 1994 Mar;35(3):458-67 Authors: Kenyon MA, Hamilton JA Binding of the medium-chain fatty acids (MCFA), octanoic (OCT) and decanoic (DEC) acid, to human serum albumin (HSA) has been studied by 13C NMR spectroscopy. NMR spectra at 35 degrees C showed an apparently homogeneous binding environment (a single, narrow resonance for the 13C-enriched carboxyl...	nmrlearner	Journal club	0	08-22-2010 03:33 AM
[NMR paper] 13C NMR studies of the binding of medium-chain fatty acids to human serum albumin. 13C NMR studies of the binding of medium-chain fatty acids to human serum albumin. Related Articles 13C NMR studies of the binding of medium-chain fatty acids to human serum albumin. J Lipid Res. 1994 Mar;35(3):458-67 Authors: Kenyon MA, Hamilton JA Binding of the medium-chain fatty acids (MCFA), octanoic (OCT) and decanoic (DEC) acid, to human serum albumin (HSA) has been studied by 13C NMR spectroscopy. NMR spectra at 35 degrees C showed an apparently homogeneous binding environment (a single, narrow resonance for the 13C-enriched carboxyl...	nmrlearner	Journal club	0	08-22-2010 03:33 AM
[NMR paper] Synthesis and interaction studies of 13C labeled lactone derivatives with a model pro Synthesis and interaction studies of 13C labeled lactone derivatives with a model protein using 13C NMR. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Synthesis and interaction studies of 13C labeled lactone derivatives with a model protein using 13C NMR. Bioorg Med Chem. 1993 Nov;1(5):389-97 Authors: Franot C, Benezra C, Lepoittevin JP Two molecules 9 and 14, representatives of two series of electrophilic lactone derivatives, have been synthesized, and labeled with...	nmrlearner	Journal club	0	08-22-2010 03:01 AM
[NMR paper] Comparative studies of the interaction of human and bovine platelet factor 4 with hep Comparative studies of the interaction of human and bovine platelet factor 4 with heparin using histidine NMR resonances as spectroscopic probes. Related Articles Comparative studies of the interaction of human and bovine platelet factor 4 with heparin using histidine NMR resonances as spectroscopic probes. J Protein Chem. 1993 Jun;12(3):303-9 Authors: Talpas CJ, Lee L The pKa values of His-38 and His-50 of the heparin-binding protein, bovine platelet factor 4, are 5.6 and 6.5, respectively, as determined by 1H NMR spectroscopy. The 1H NMR...	nmrlearner	Journal club	0	08-21-2010 11:53 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off