Related Articles1H NMR studies on the interaction of beta-carboline derivatives with human serum albumin.
J Magn Reson. 1998 Feb;130(2):281-6
Authors: Veglia G, Delfini M, Giudice MR, Gaggelli E, Valensin G
1H NMR studies were performed on two beta-carboline derivatives interacting with human serum albumin. The spin-lattice relaxation rates of the two derivatives, having side chains of different length and polarity, were used to demonstrate a diverse motional behavior in solution together with slightly different relaxation pathways. Single- and double-selective excitation made it possible to evaluate dynamics in the free and protein-bound states. Occurrence of a relatively long hydrophilic chain interacting with the proton-acceptor nitrogen of the beta-carboline moiety was shown to yield lower association constants, slower dissociation rates, and diverse interacting modes with the indole hydrophobic site of the protein.
[NMR900 blog] The Human Serum Metabolome
The Human Serum Metabolome
N. Psychogios, D.D. Hau, J. Peng, A.C. Guo, R. Mandal, S. Bouatra, I. Sinelnikov, R. Krishnamurthy, R. Eisner, B. Gautam, N. Young, J. Xia, C. Knox, E. Dong, P. Huang, Z. Hollander, T.L. Pedersen, S.R. Smith, F. Bamforth, R. Greiner, B. McManus, J.W. Newman, T. Goodfriend, D.S. Wishart, "The Human Serum Metabolome," PLoS ONE 6 (2011) e16957. (open access article) http://dx.doi.org/10.1371/journal.pone.0016957
Abstract: Continuing improvements in analytical technology along with an increased interest in performing comprehensive, quantitative metabolic...
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02-17-2011 09:44 PM
[NMR paper] Determination of protein-ligand binding affinity by NMR: observations from serum albu
Determination of protein-ligand binding affinity by NMR: observations from serum albumin model systems.
Related Articles Determination of protein-ligand binding affinity by NMR: observations from serum albumin model systems.
Magn Reson Chem. 2005 Jun;43(6):463-70
Authors: Fielding L, Rutherford S, Fletcher D
The usefulness of bovine serum albumin (BSA) as a model protein for testing NMR methods for the study of protein-ligand interactions is discussed. Isothermal titration calorimetry established the binding affinity and stoichiometry of the...
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11-25-2010 08:21 PM
[NMR paper] Structural studies of the putative helix 8 in the human beta(2) adrenergic receptor:
Structural studies of the putative helix 8 in the human beta(2) adrenergic receptor: an NMR study.
Related Articles Structural studies of the putative helix 8 in the human beta(2) adrenergic receptor: an NMR study.
Biochim Biophys Acta. 2004 May 27;1663(1-2):74-81
Authors: Katragadda M, Maciejewski MW, Yeagle PL
The recently reported crystal structure of bovine rhodopsin revealed a cytoplasmic helix (helix 8) in addition to the seven transmembrane helices. This domain is roughly perpendicular to the transmembrane bundle in the presence of an...
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11-24-2010 09:51 PM
[NMR paper] Interaction between beta-Purothionin and dimyristoylphosphatidylglycerol: a (31)P-NMR
Interaction between beta-Purothionin and dimyristoylphosphatidylglycerol: a (31)P-NMR and infrared spectroscopic study.
Related Articles Interaction between beta-Purothionin and dimyristoylphosphatidylglycerol: a (31)P-NMR and infrared spectroscopic study.
Biophys J. 2002 Oct;83(4):2074-83
Authors: Richard JA, Kelly I, Marion D, Pézolet M, Auger M
The interaction of beta-purothionin, a small basic and antimicrobial protein from the endosperm of wheat seeds, with multilamellar vesicles of dimyristoylphosphatidylglycerol (DMPG) was investigated...
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11-24-2010 08:58 PM
[NMR paper] 13C NMR studies of the binding of medium-chain fatty acids to human serum albumin.
13C NMR studies of the binding of medium-chain fatty acids to human serum albumin.
Related Articles 13C NMR studies of the binding of medium-chain fatty acids to human serum albumin.
J Lipid Res. 1994 Mar;35(3):458-67
Authors: Kenyon MA, Hamilton JA
Binding of the medium-chain fatty acids (MCFA), octanoic (OCT) and decanoic (DEC) acid, to human serum albumin (HSA) has been studied by 13C NMR spectroscopy. NMR spectra at 35 degrees C showed an apparently homogeneous binding environment (a single, narrow resonance for the 13C-enriched carboxyl...
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08-22-2010 03:33 AM
[NMR paper] 13C NMR studies of the binding of medium-chain fatty acids to human serum albumin.
13C NMR studies of the binding of medium-chain fatty acids to human serum albumin.
Related Articles 13C NMR studies of the binding of medium-chain fatty acids to human serum albumin.
J Lipid Res. 1994 Mar;35(3):458-67
Authors: Kenyon MA, Hamilton JA
Binding of the medium-chain fatty acids (MCFA), octanoic (OCT) and decanoic (DEC) acid, to human serum albumin (HSA) has been studied by 13C NMR spectroscopy. NMR spectra at 35 degrees C showed an apparently homogeneous binding environment (a single, narrow resonance for the 13C-enriched carboxyl...
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[NMR paper] Synthesis and interaction studies of 13C labeled lactone derivatives with a model pro
Synthesis and interaction studies of 13C labeled lactone derivatives with a model protein using 13C NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Synthesis and interaction studies of 13C labeled lactone derivatives with a model protein using 13C NMR.
Bioorg Med Chem. 1993 Nov;1(5):389-97
Authors: Franot C, Benezra C, Lepoittevin JP
Two molecules 9 and 14, representatives of two series of electrophilic lactone derivatives, have been synthesized, and labeled with...
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08-22-2010 03:01 AM
[NMR paper] Comparative studies of the interaction of human and bovine platelet factor 4 with hep
Comparative studies of the interaction of human and bovine platelet factor 4 with heparin using histidine NMR resonances as spectroscopic probes.
Related Articles Comparative studies of the interaction of human and bovine platelet factor 4 with heparin using histidine NMR resonances as spectroscopic probes.
J Protein Chem. 1993 Jun;12(3):303-9
Authors: Talpas CJ, Lee L
The pKa values of His-38 and His-50 of the heparin-binding protein, bovine platelet factor 4, are 5.6 and 6.5, respectively, as determined by 1H NMR spectroscopy. The 1H NMR...