NMR paper 1H NMR Self-Diffusion in Polymer-Surfactant Nanocapsules and Cryogels with Enzyme.

		BioNMR > Educational resources > Journal club
[NMR paper] 1H NMR Self-Diffusion in Polymer-Surfactant Nanocapsules and Cryogels with Enzyme.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-17-2010, 11:15 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,574

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

1H NMR Self-Diffusion in Polymer-Surfactant Nanocapsules and Cryogels with Enzyme.

1H NMR Self-Diffusion in Polymer-Surfactant Nanocapsules and Cryogels with Enzyme.

Related Articles 1H NMR Self-Diffusion in Polymer-Surfactant Nanocapsules and Cryogels with Enzyme.

J Colloid Interface Sci. 1998 Oct 1;206(1):168-176

Authors: Shapiro YE, Pykhteeva EG, Levashov AV

The multicomponent self-diffusion in nanocapsules and cryogel biocatalytic systems containing alpha-chymotrypsin has been studied with the NMR-PGSE method at various temperatures and compared with the diffusion of such systems without enzyme. Unilamellar vesicles have been formed in water after "coating" with Brij-97 of the poly-(N,N-diallyl-N,N-didodecyl ammonium bromide), poly-DDAB, nanocapsules. The latter have been obtained by UV-irradiation of reversed hydrated micelles from DDAB in cyclohexane. Cryogels were made from poly(vinyl alcohol), PVA, aqueous solutions by a freezing-thawing cyclic process. Both compartmented systems were used as vehicles of the enzyme entrapped in inner aqueous cavities. The activation energies of self-diffusion for both these systems have been calculated. These data contain information concerning morphology and molecular packing. Encapsulation of alpha-chymotrypsin in the poly-DDAB/Brij-97 vesicles and the PVA cryogel lowers the Ds values for all molecules and shifts the cloud point toward the lower temperature. On the contrary, the syneresis point for the PVA cryogel is shifted for 8 degrees toward the higher temperature by the entrapment of the enzyme. Besides, entrapment of alpha-chymotrypsin in the cryogel promotes the increase of the Ea values for the PVA chain on 1.5 kJ/mol below the syneresis point. Such a difference indicates the influence of the H-bond system of PVA hydroxyl groups and water molecules on the interference of the protein globule. Entrapment of alpha-chymotrypsin leads to consolidation of this H-bond system. Copyright 1998 Academic Press.

PMID: 9761640 [PubMed - as supplied by publisher]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Li Ion Diffusion in the Anode Material Li12Si7: Ultrafast Quasi-1D Diffusion and Two Distinct Fast 3D Jump Processes Separately Revealed by 7Li NMR Relaxometry Li Ion Diffusion in the Anode Material Li12Si7: Ultrafast Quasi-1D Diffusion and Two Distinct Fast 3D Jump Processes Separately Revealed by 7Li NMR Relaxometry Alexander Kuhn, Puravankara Sreeraj, Rainer Po?ttgen, Hans-Dieter Wiemho?fer, Martin Wilkening and Paul Heitjans http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja2020108/aop/images/medium/ja-2011-020108_0005.gif Journal of the American Chemical Society DOI: 10.1021/ja2020108 http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA...	nmrlearner	Journal club	0	06-28-2011 04:32 AM
[NMR paper] NMR spectroscopy of proteins encapsulated in a positively charged surfactant. NMR spectroscopy of proteins encapsulated in a positively charged surfactant. Related Articles NMR spectroscopy of proteins encapsulated in a positively charged surfactant. J Magn Reson. 2005 Jul;175(1):158-62 Authors: Lefebvre BG, Liu W, Peterson RW, Valentine KG, Wand AJ Traditionally, large proteins, aggregation-prone proteins, and membrane proteins have been difficult to examine by modern multinuclear and multidimensional solution NMR spectroscopy. A major limitation presented by these protein systems is that their slow molecular...	nmrlearner	Journal club	0	11-25-2010 08:21 PM
[NMR paper] NMR structure of lung surfactant peptide SP-B(11-25). NMR structure of lung surfactant peptide SP-B(11-25). Related Articles NMR structure of lung surfactant peptide SP-B(11-25). Biochemistry. 2002 Jul 30;41(30):9627-36 Authors: Kurutz JW, Lee KY Surfactant protein B (SP-B) is a 79-residue essential component of lung surfactant, the film of lipid and protein lining the alveoli, and is the subject of great interest for its role in lung surfactant replacement therapies. Here we report circular dichroism results and the solution NMR structure of SP-B(11-25) (CRALIKRIQAMIPKG) dissolved in CD(3)OH at...	nmrlearner	Journal club	0	11-24-2010 08:58 PM
[NMR paper] What NMR can tell us about where lung surfactant proteins live. What NMR can tell us about where lung surfactant proteins live. Related Articles What NMR can tell us about where lung surfactant proteins live. Biochem Soc Trans. 1997 Aug;25(3):1103-7 Authors: Morrow MR, Taneva S, Dico AS, Hancock J, Keough KM 2H-NMR is beginning to provide some insights into the way in which the hydrophobic surfactant proteins SP-B and SP-C interact with phospholipid bilayers in multilamellar structures. Both proteins have a significant effect on slow bilayer motions. In many ways, the effect of SP-C on the surrounding...	nmrlearner	Journal club	0	08-22-2010 05:08 PM
[NMR paper] The NMR structure of the pulmonary surfactant-associated polypeptide SP-C in an apola The NMR structure of the pulmonary surfactant-associated polypeptide SP-C in an apolar solvent contains a valyl-rich alpha-helix. Related Articles The NMR structure of the pulmonary surfactant-associated polypeptide SP-C in an apolar solvent contains a valyl-rich alpha-helix. Biochemistry. 1994 May 17;33(19):6015-23 Authors: Johansson J, Szyperski T, Curstedt T, WÃ¼thrich K The nuclear magnetic resonance (NMR) structure of the pulmonary surfactant-associated lipoplypeptide C (SP-C) was determined in a mixed solvent of C2H3Cl/C2H3OH/ 1 M HCl...	nmrlearner	Journal club	0	08-22-2010 03:33 AM
[NMR paper] The NMR structure of the pulmonary surfactant-associated polypeptide SP-C in an apola The NMR structure of the pulmonary surfactant-associated polypeptide SP-C in an apolar solvent contains a valyl-rich alpha-helix. Related Articles The NMR structure of the pulmonary surfactant-associated polypeptide SP-C in an apolar solvent contains a valyl-rich alpha-helix. Biochemistry. 1994 May 17;33(19):6015-23 Authors: Johansson J, Szyperski T, Curstedt T, WÃ¼thrich K The nuclear magnetic resonance (NMR) structure of the pulmonary surfactant-associated lipoplypeptide C (SP-C) was determined in a mixed solvent of C2H3Cl/C2H3OH/ 1 M HCl...	nmrlearner	Journal club	0	08-22-2010 03:33 AM
[NMR paper] 2H NMR studies of the effect of pulmonary surfactant SP-C on the 1,2-dipalmitoyl-sn-g 2H NMR studies of the effect of pulmonary surfactant SP-C on the 1,2-dipalmitoyl-sn-glycero-3-phosphocholine headgroup: a model for transbilayer peptides in surfactant and biological membranes. Related Articles 2H NMR studies of the effect of pulmonary surfactant SP-C on the 1,2-dipalmitoyl-sn-glycero-3-phosphocholine headgroup: a model for transbilayer peptides in surfactant and biological membranes. Biochemistry. 1993 Oct 26;32(42):11338-44 Authors: Morrow MR, Taneva S, Simatos GA, Allwood LA, Keough KM Surfactant protein C (SP-C) was...	nmrlearner	Journal club	0	08-22-2010 03:01 AM
Self-diffusion in Polymer Systems studied by Magnetic Field-Gradient Spin-Echo NMR Me Self-diffusion in Polymer Systems studied by Magnetic Field-Gradient Spin-Echo NMR Methods Publication year: 2010 Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 13 April 2010</br> Harald, Walderhaug , Olle, Söderman , Daniel, Topgaard</br> More...	nmrlearner	Journal club	0	08-16-2010 03:50 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off