NMR paper 1H NMR relaxation investigation of acetylcholinesterase inhibitors from huperzine A a

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[NMR paper] 1H NMR relaxation investigation of acetylcholinesterase inhibitors from huperzine A a

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11-24-2010, 09:25 PM

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1H NMR relaxation investigation of acetylcholinesterase inhibitors from huperzine A a

1H NMR relaxation investigation of acetylcholinesterase inhibitors from huperzine A and derivative.

Related Articles 1H NMR relaxation investigation of acetylcholinesterase inhibitors from huperzine A and derivative.

Bioorg Med Chem Lett. 2004 Mar 22;14(6):1585-8

Authors: Li Y, Li Q, Sun M, Song G, Jiang S, Zhu D

The binding properties of huperzine A (1) with Torpediniforms Nacline acetylcholinesterase (TnAChE) were investigated by (1)H NMR methods. The noselective, selective and double-selective spin-lattice relaxation rates were acquired in absent and present of TnAChE at a ratio [ligand]/[protein]=1:0.005. The selective relaxation rates shown protons of 1 had dipole-dipole interaction with protein active site protons. The motional correlation time of bound ligand was calculated by double-selective relaxation rate at 1 tau(2,3)=40.5 ns at 298 K, which showed 1 had high affinity with TnAChE. The experiments give a possible method to use TnAChE to locate the new huperzine A derivatives as AChE inhibitors.

PMID: 15006409 [PubMed - indexed for MEDLINE]

Source: PubMed

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