[NMR paper] 1H NMR Metabolomics Reveals Association of High Expression of Inositol 1, 4, 5 Trisphosphate Receptor and Metabolites in Breast Cancer Patients.
Related Articles1H NMR Metabolomics Reveals Association of High Expression of Inositol 1, 4, 5 Trisphosphate Receptor and Metabolites in Breast Cancer Patients.
PLoS One. 2017;12(1):e0169330
Authors: Singh A, Sharma RK, Chagtoo M, Agarwal G, George N, Sinha N, Godbole MM
Abstract
1H NMR is used to detect alterations in metabolites and their linkage to metabolic processes in a number of pathological conditions including breast cancer. Inositol 1, 4, 5 trisphosphate (IP3R) receptor is an intracellular calcium channel known to regulate metabolism and cellular bioenergetics. Its expression is up regulated in a number of cancers. However, its linkage to metabolism in disease conditions has not been evaluated. This study was designed to determine the association if any, of these metabolites with altered expression of IP3R in breast cancer. We used 1H NMR to identify metabolites in the serum of breast cancer patients (n = 27) and performed Real-time Polymerase Chain Reaction analysis for quantifying the expression of IP3R type 3 and type 2 in tissues from breast cancer patients (n = 40). Principal Component Analysis (PCA) and Partial Least Square-Discriminant Analysis (PLS-DA) clearly distinguished patients with high/low IP3R expression from healthy subjects. The present study revealed high expression of IP3R type 2 and type 3 in human breast tumor tissue compared to adjacent non-tumorous tissue. Moreover, patients with >= 2-fold increase in IP3R (high IP3R group) had significantly higher concentration of metabolic intermediates compared to those with < 2-fold increase in IP3R (low IP3R group). We observed an increase in lipoprotein content and the levels of metabolites like lactate, lysine and alanine and a decrease in the levels of pyruvate and glucose in serum of high IP3R group patients when compared to those in healthy subjects. Receiver operating characteristic (ROC) curve analysis was performed to show the clinical utility of metabolites. In addition to the human studies, functional relevance of IP3Rs in causing metabolic disruption was observed in MCF-7 and MDA MB-231 cells. Results from our studies bring forth the importance of metabolic (or metabolomics) profiling of serum by 1H NMR in conjunction with tissue expression studies for characterizing breast cancer patients. The results from this study provide new insights into relationship of breast cancer metabolites with IP3R.
[NMR paper] (1)H NMR-based metabolomics studies of urine reveal differences between type 1 diabetic patients with high and low HbAc1 values.
(1)H NMR-based metabolomics studies of urine reveal differences between type 1 diabetic patients with high and low HbAc1 values.
(1)H NMR-based metabolomics studies of urine reveal differences between type 1 diabetic patients with high and low HbAc1 values.
J Pharm Biomed Anal. 2013 Apr 22;83C:43-48
Authors: Deja S, Barg E, M?ynarz P, Basiak A, Willak-Janc E
Abstract
The aim of this study was to investigate relation between level of HbAc1 and concentration of metabolites in urine of T1D patients. To test this hypothesis the (1)H NMR...
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05-25-2013 12:05 PM
1H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system
1H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system
Abstract Hypoxia can promote invasive behavior in cancer cells and alters the response to therapeutic intervention as a result of changes in the expression many genes, including genes involved in intermediary metabolism. Although metabolomics technologies are capable of simultaneously measuring a wide range of metabolites in an untargeted manner, these methods have been relatively under utilized in the study of cancer cell responses to hypoxia. Thus, 1H NMR metabolomics was...
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03-09-2011 04:19 AM
(1)H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system.
(1)H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system.
(1)H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system.
J Biomol NMR. 2011 Mar 4;
Authors: Weljie AM, Bondareva A, Zang P, Jirik FR
Hypoxia can promote invasive behavior in cancer cells and alters the response to therapeutic intervention as a result of changes in the expression many genes, including genes involved in intermediary metabolism. Although metabolomics...
1H NMR Metabolomics Reveals Association of High Expression of Inositol 1, 4, 5 Trisphosphate Receptor and Metabolites in Breast Cancer Patients.
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