[NMR paper] 1H NMR metabolic profiling of cod (Gadus morhua) larvae: potential effects of temperature and diet composition during early developmental stages.
Related Articles1H NMR metabolic profiling of cod (Gadus morhua) larvae: potential effects of temperature and diet composition during early developmental stages.
Biol Open. 2015 Nov 6;
Authors: Chauton MS, Galloway TF, Kjørsvik E, Størseth TR, Puvanendran V, van der Meeren T, Karlsen Ø, Rønnestad I, Hamre K
Abstract
Marine aquaculture offers a great source of protein for the increasing human population, and farming of, for example, Atlantic salmon is a global industry. Atlantic cod farming however, is an example of a promising industry where the potential is not yet realized. Research has revealed that a major bottleneck to successful farming of cod is poor quality of the larvae and juveniles. A large research program was designed to increase our understanding of how environmental factors such as temperature and nutrition affects cod larvae development. Data on larvae growth and development were used together with nuclear magnetic resonance. The NMR data indicated that the temperature influenced the metabolome of the larvae; differences were related to osmolytes such as betaine/TMAO, the amino acid taurine, and creatine and lactate which reflect muscle activity. The larvae were fed Artemia from stage 2, and this was probably reflected in a high taurine content of older larvae. Larvae fed with copepods in the nutrition experiment also displayed a high taurine content, together with higher creatine and betaine/TMAO content. Data on the cod larvae metabolome should be coupled to data on gene expression, in order to identify events which are regulated on the genetic level versus regulation resulting from temperature or nutrition during development, to fully understand how the environment affects larval development.
PMID: 26545964 [PubMed - as supplied by publisher]
[NMR paper] Effects of solvent concentration and composition on protein dynamics: 13C MAS NMR studies of elastin in glycerol-water mixtures.
Effects of solvent concentration and composition on protein dynamics: 13C MAS NMR studies of elastin in glycerol-water mixtures.
Related Articles Effects of solvent concentration and composition on protein dynamics: 13C MAS NMR studies of elastin in glycerol-water mixtures.
Biochim Biophys Acta. 2015 Apr 24;
Authors: Demuth D, Haase N, Malzacher D, Vogel M
Abstract
We use 13C CP MAS NMR to investigate the dependence of elastin dynamics on the concentration and composition of the solvent at various temperatures. For elastin in...
nmrlearner
Journal club
0
04-29-2015 03:49 PM
Effects of solvent concentration and composition on protein dynamics: 13C MAS NMR studies of elastin in glycerol-water mixtures
Effects of solvent concentration and composition on protein dynamics: 13C MAS NMR studies of elastin in glycerol-water mixtures
Publication date: Available online 25 April 2015
Source:Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics</br>
Author(s): Dominik Demuth , Nils Haase , Daniel Malzacher , Michael Vogel</br>
We use 13C CP MAS NMR to investigate the dependence of elastin dynamics on the concentration and composition of the solvent at various temperatures. For elastin in pure glycerol, line-shape analysis shows that larger-scale fluctuations of...
nmrlearner
Journal club
0
04-26-2015 03:28 AM
[NMR paper] Optimization of Bicelle Lipid Composition and Temperature for EPR Spectroscopy of Aligned Membranes
Optimization of Bicelle Lipid Composition and Temperature for EPR Spectroscopy of Aligned Membranes
Publication date: Available online 13 October 2014
Source:Journal of Magnetic Resonance</br>
Author(s): Jesse E. McCaffrey , Zachary M. James , David D. Thomas</br>
We have optimized the magnetic alignment of phospholipid bilayered micelles (bicelles) for EPR spectroscopy, by varying lipid composition and temperature. Bicelles have been extensively used in NMR spectroscopy for several decades, in order to obtain aligned samples in a near-native membrane environment...
nmrlearner
Journal club
0
11-14-2014 08:33 AM
Colon cancer calculated: Metabolic profiling
Colon cancer calculated: Metabolic profiling
http://www.spectroscopynow.com/common/images/thumbnails/14077d20717.jpgNuclear magnetic resonance (NMR) spectroscopy can be used to obtain a metabolic fingerprint of cancer of the bowel and so determine how far advanced the disease is, according to UK researchers.
Read the rest at Spectroscopynow.com
nmrlearner
General
0
08-15-2013 07:22 AM
Metabolic profiling of cadmium-induced effects in one pioneer intertidal halophyte Suaeda salsa by NMR-based metabolomics.
Metabolic profiling of cadmium-induced effects in one pioneer intertidal halophyte Suaeda salsa by NMR-based metabolomics.
Metabolic profiling of cadmium-induced effects in one pioneer intertidal halophyte Suaeda salsa by NMR-based metabolomics.
Ecotoxicology. 2011 May 15;
Authors: Liu X, Yang C, Zhang L, Li L, Liu S, Yu J, You L, Zhou D, Xia C, Zhao J, Wu H
Cadmium is a non-essential element to living organisms and has become the severe contaminant in both seawater and sediment in the intertidal zones of the Bohai Sea. The halophyte, Suaeda salsa...
nmrlearner
Journal club
0
05-17-2011 06:21 PM
[NMR paper] In vivo 13C NMR metabolite profiling: potential for understanding and assessing conifer seed quality.
In vivo 13C NMR metabolite profiling: potential for understanding and assessing conifer seed quality.
Related Articles In vivo 13C NMR metabolite profiling: potential for understanding and assessing conifer seed quality.
J Exp Bot. 2005 Aug;56(418):2253-65
Authors: Terskikh VV, Feurtado JA, Borchardt S, Giblin M, Abrams SR, Kermode AR
High-resolution 13C MAS NMR spectroscopy was used to profile a range of primary and secondary metabolites in vivo in intact whole seeds of eight different conifer species native to North America, including six of...
nmrlearner
Journal club
0
12-01-2010 06:56 PM
[NMR paper] The solution NMR structure of glucosylated N-glycans involved in the early stages of
The solution NMR structure of glucosylated N-glycans involved in the early stages of glycoprotein biosynthesis and folding.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles The solution NMR structure of glucosylated N-glycans involved in the early stages of glycoprotein biosynthesis and folding.
EMBO J. 1997 Jul 16;16(14):4302-10
Authors: Petrescu AJ, Butters TD, Reinkensmeier G, Petrescu S, Platt FM, Dwek RA, Wormald MR
Glucosylated oligomannose N-linked...
1H NMR metabolic profiling of cod (Gadus morhua) larvae: potential effects of temperature and diet composition during early developmental stages.
Linear Mode
