NMR paper 1H NMR-based absolute quantitation of human lipoproteins and their lipid contents dir

		BioNMR > Educational resources > Journal club
[NMR paper] 1H NMR-based absolute quantitation of human lipoproteins and their lipid contents dir

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-22-2010, 03:29 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,714

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

1H NMR-based absolute quantitation of human lipoproteins and their lipid contents dir

1H NMR-based absolute quantitation of human lipoproteins and their lipid contents directly from plasma.

Related Articles 1H NMR-based absolute quantitation of human lipoproteins and their lipid contents directly from plasma.

J Lipid Res. 1994 Dec;35(12):2292-304

Authors: Ala-Korpela M, Korhonen A, Keisala J, HÃ¶rkkÃ¶ S, Korpi P, Ingman LP, Jokisaari J, Savolainen MJ, KesÃ¤niemi YA

A new method is presented for absolute quantitation of lipid and protein contents of human lipoproteins directly from plasma. The method enables complete lipoprotein lipid profiles to be obtained in a total time of less than one hour. Absolute concentrations of triglycerides, phospholipids, total cholesterol, free cholesterol, esterified cholesterol, total proteins, and total masses can be estimated for the very low density (VLDL) and low density (LDL) lipoprotein fractions. For the high density lipoprotein (HDL) fraction all components except triglycerides can be quantitated. The method is a combination of 1H NMR spectroscopy and a sophisticated lineshape fitting analysis technique. In this paper we present the calibration of the method using 15 plasma samples followed by a double-blind test of 51 plasma samples from 43 individuals. In total, 66 plasma samples were analyzed. Comparison of the 1H NMR-based results with the data of the biochemical assays showed excellent agreement; the correlation coefficient for VLDL triglycerides was 0.98, for LDL cholesterol 0.88, and for HDL cholesterol 0.93. This method can be directly integrated to many kinds of biomedical NMR studies to offer additional biochemically important quantitative lipoprotein information, the measurement of which is usually too laborious by conventional biochemical methods and too high-priced to be adapted into the study protocols. Moreover, the method also has considerable potential to be developed for a routine clinical assay.

PMID: 7897326 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] GFT NMR based resonance assignment for the 21 kDa human protein UFC1. GFT NMR based resonance assignment for the 21 kDa human protein UFC1. Related Articles GFT NMR based resonance assignment for the 21 kDa human protein UFC1. J Biomol NMR. 2005 Jul;32(3):261 Authors: Liu G, Aramini J, Atreya HS, Eletsky A, Xiao R, Acton T, Ma L, Montelione GT, Szyperski T	nmrlearner	Journal club	0	12-01-2010 06:56 PM
[NMR paper] Lipid analysis of human HDL and LDL by MALDI-TOF mass spectrometry and (31)P-NMR. Lipid analysis of human HDL and LDL by MALDI-TOF mass spectrometry and (31)P-NMR. Related Articles Lipid analysis of human HDL and LDL by MALDI-TOF mass spectrometry and (31)P-NMR. J Lipid Res. 2001 Sep;42(9):1501-8 Authors: Schiller J, Zschörnig O, Petkovi? M, Müller M, Arnhold J, Arnold K The analysis of HDL and LDL is important for the further understanding of atherosclerosis because changes of the protein and lipid moieties occur under pathological conditions. Because destruction of lipids leads to the formation of well-defined products...	nmrlearner	Journal club	0	11-19-2010 08:44 PM
Cu and Fe metallic ions-mediated oxidation of low-density lipoproteins studied by NMR Cu and Fe metallic ions-mediated oxidation of low-density lipoproteins studied by NMR, TEM and Z-scan technique. Related Articles Cu and Fe metallic ions-mediated oxidation of low-density lipoproteins studied by NMR, TEM and Z-scan technique. Chem Phys Lipids. 2010 Jun;163(6):545-51 Authors: Gómez SL, Monteiro AM, Rabbani SR, Bloise AC, Carneiro SM, Alves S, Gidlund M, Abdalla DS, Neto AM In this work we report on a study of the morphological changes of LDL induced in vitro by metallic ions (Cu(2+) and Fe(3+)). These modifications were...	nmrlearner	Journal club	0	10-22-2010 06:02 AM
[NMR paper] NMR-based discovery of lead inhibitors that block DNA binding of the human papillomav NMR-based discovery of lead inhibitors that block DNA binding of the human papillomavirus E2 protein. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles NMR-based discovery of lead inhibitors that block DNA binding of the human papillomavirus E2 protein. J Med Chem. 1997 Sep 26;40(20):3144-50 Authors: Hajduk PJ, Dinges J, Miknis GF, Merlock M, Middleton T, Kempf DJ, Egan DA, Walter KA, Robins TS, Shuker SB, Holzman TF, Fesik SW The E2 protein is required for the replication of human...	nmrlearner	Journal club	0	08-22-2010 05:08 PM
[NMR paper] A model for human cytochrome P450 2D6 based on homology modeling and NMR studies of s A model for human cytochrome P450 2D6 based on homology modeling and NMR studies of substrate binding. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles A model for human cytochrome P450 2D6 based on homology modeling and NMR studies of substrate binding. Biochemistry. 1996 Apr 9;35(14):4540-50 Authors: Modi S, Paine MJ, Sutcliffe MJ, Lian LY, Primrose WU, Wolf CR, Roberts GC The cytochrome P450 responsible for the debrisoquine/sparteine polymorphism (P450 2D6) has been produced in large...	nmrlearner	Journal club	0	08-22-2010 02:27 PM
[NMR paper] 1H NMR-based determination of the three-dimensional structure of the human plasma fib 1H NMR-based determination of the three-dimensional structure of the human plasma fibronectin fragment containing inter-chain disulfide bonds. Related Articles 1H NMR-based determination of the three-dimensional structure of the human plasma fibronectin fragment containing inter-chain disulfide bonds. J Biol Chem. 1993 Apr 25;268(12):8580-9 Authors: Kar L, Lai CS, Wolff CE, Nettesheim D, Sherman S, Johnson ME Human plasma fibronectin is a plasma glycoprotein that plays an important role in many biological processes. It consists of two...	nmrlearner	Journal club	0	08-21-2010 11:53 PM
[NMR paper] Identification of trapped and boundary lipid binding sites in M13 coat protein/lipid Identification of trapped and boundary lipid binding sites in M13 coat protein/lipid complexes by deuterium NMR spectroscopy. Related Articles Identification of trapped and boundary lipid binding sites in M13 coat protein/lipid complexes by deuterium NMR spectroscopy. Biochemistry. 1990 Apr 24;29(16):3828-34 Authors: Van Gorkom LC, HorvÃ¡th LI, Hemminga MA, Sternberg B, Watts A The major coat protein of M13 bacteriophage has been incorporated into bilayers of 1,2-dimyristoyl-sn-glycero-3-phosphocholine, deuterated in the trimethyl segments of...	nmrlearner	Journal club	0	08-21-2010 10:48 PM
[NMR paper] Dynamic structure of the lower density lipoproteins. II. Deuterium NMR studies of the Dynamic structure of the lower density lipoproteins. II. Deuterium NMR studies of the monolayer of very low and low density lipoproteins. Related Articles Dynamic structure of the lower density lipoproteins. II. Deuterium NMR studies of the monolayer of very low and low density lipoproteins. Biochem Cell Biol. 1990 Jan;68(1):189-98 Authors: Chana RS, Treleaven WD, Parmar YI, Cushley RJ The order of phosphatidylcholine (PC) acyl chains in the surface monolayer of very low density lipoproteins (VLDL) and low density lipoproteins (LDL) has been...	nmrlearner	Journal club	0	08-21-2010 10:48 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off