NMR paper 1H-NMR assignments and local environments of aromatic residues in bovine, human and g

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[NMR paper] 1H-NMR assignments and local environments of aromatic residues in bovine, human and g

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08-21-2010, 11:45 PM

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1H-NMR assignments and local environments of aromatic residues in bovine, human and g

1H-NMR assignments and local environments of aromatic residues in bovine, human and guinea pig variants of alpha-lactalbumin.

Related Articles 1H-NMR assignments and local environments of aromatic residues in bovine, human and guinea pig variants of alpha-lactalbumin.

Eur J Biochem. 1992 Dec 15;210(3):699-709

Authors: Alexandrescu AT, Broadhurst RW, Wormald C, Chyan CL, Baum J, Dobson CM

1H-NMR assignments have been defined for the aromatic-ring protons of the bovine, guinea pig and human variants of alpha-lactalbumin. Spin-system networks were identified by means of double-quantum-filtered two-dimensional J-correlated spectroscopy and two-dimensional relayed coherence spectroscopy data. Analysis of two-dimensional nuclear-Overhauser-enhancement spectroscopy data of the proteins indicated that in each case two clusters of aromatic residues exist. The two clusters are also evident in the crystal structure of the human protein, and this evidence, in conjunction with sequence differences between the three proteins, permitted sequence-specific assignments to be made for the majority of aromatic residues. Remaining ambiguities in the assignments could be resolved by analysis of photochemically induced dynamic nuclear polarization (PCIDNP) effects. Comparison of the PCIDNP spectra of the three proteins indicated the presence of only minor differences in the surface exposure of conserved aromatic residues. Taken together, these results indicate that the environments of the conserved aromatic residues in bovine, guinea pig and human alpha-lactalbumin in solution are very similar to each other, and that the solution and the crystal forms of at least the human protein are similar.

PMID: 1483454 [PubMed - indexed for MEDLINE]

Source: PubMed

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