Related Articles1H(C) and 1H(N) total NOE correlations in a single 3D NMR experiment. 15N and 13C time-sharing in t1 and t2 dimensions for simultaneous data acquisition.
J Biomol NMR. 2003 Nov;27(3):193-203
Authors: Xia Y, Yee A, Arrowsmith CH, Gao X
Simultaneous data acquisition in time-sharing (TS) multi-dimensional NMR experiments has been shown an effective means to reduce experimental time, and thus to accelerate structure determination of proteins. This has been accomplished by spin evolution time-sharing of the X and Y heteronuclei, such as (15)N and (13)C, in one of the time dimensions. In this work, we report a new 3D TS experiment, which allows simultaneous (13)C and (15)N spin labeling coherence in both t(1) and t(2) dimensions to give four NOESY spectra in a single 3D experiment. These spectra represent total NOE correlations between (1)H(N) and (1)H(C) resonances. This strategy of double time-sharing (2TS) results in an overall four-fold reduction in experimental time compared with its conventional counterpart. This 3D 2TS CN-CN-H HSQC-NOESY-HSQC pulse sequence also demonstrates improvements in water suppression, (15)N spectral resolution and sensitivity, which were developed based on 2D TS CN-H HSQC and 3D TS H-CN-H NOESY-HSQC experiments. Combining the 3D TS and the 3D 2TS NOESY experiments, NOE assignment ambiguities and errors are considerably reduced. These results will be useful for rapid protein structure determination to complement the effort of discerning the functions of diverse genomic proteins.
Cross-Correlations Between Low-? Nuclei in Solids Via a Common Dipolar Bath
Cross-Correlations Between Low-? Nuclei in Solids Via a Common Dipolar Bath
Publication year: 2011
Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 20 July 2011</br>
Aanatoly K., Khitrin , Jiadi, Xu , Ayyalusamy, Ramamoorthy</br>
Correlation of chemical shifts of low-? nuclei (such as 15N) is an important method for assignment of resonances in uniformly-labeled biological solids. Under static experimental conditions, an efficient mixing of low-? nuclear spin magnetization can be achieved by a thermal contact to the common reservoir of...
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07-21-2011 10:31 PM
[Question from NMRWiki Q&A forum] Correlations across glycosidic bonds
Correlations across glycosidic bonds
I am studying carbohydrates and was wanting to know if it was possible to record correlations through the ether linkage of the glycosidic bond in sugars either from the proton or carbon of one sugar top the adjacent sugar or the protons of a Serine or threonine side chain in a glyco-peptide. Would something like a 13C-HSQC-TOCSY cross the glycosidic bond? if not are there experiments that would?
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03-19-2011 07:01 AM
[Question from NMRWiki Q&A forum] From what experiment only two bond correlations we can get in varian nmr?
From what experiment only two bond correlations we can get in varian nmr?
Hi., i have one compound i need only two bond correlation contours from quaternary carbon to proton. Is there any pulse sequence to find only two bond correlations ie, from quaternary carbon to protons with minimum amount of sample in "varian nmr".
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12-28-2010 05:27 AM
[NMR paper] Detergent-resistant membrane fractions contribute to the total 1H NMR-visible lipid s
Detergent-resistant membrane fractions contribute to the total 1H NMR-visible lipid signal in cells.
Related Articles Detergent-resistant membrane fractions contribute to the total 1H NMR-visible lipid signal in cells.
Eur J Biochem. 2003 May;270(9):2091-100
Authors: Wright LC, Djordjevic JT, Schibeci SD, Himmelreich U, Muljadi N, Williamson P, Lynch GW
Leukocytes and other cells show an enhanced intensity of mobile lipid in their 1H NMR spectra under a variety of conditions. Such conditions include stimulation, which has recently been shown...
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11-24-2010 09:01 PM
Analytical Chemist, Spectroscopy | TOTAL Petrochemicals USA, Inc.
Analytical Chemist, Spectroscopy | TOTAL Petrochemicals USA, Inc.
US - Nationwide, Advanced degree or equivalent experience in Chemistry or related fieldUnderstanding and experience in NMR and FTIRExcellent communication skillsDemonstrated success in working as part of a team
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11-22-2010 07:08 PM
[NMR paper] NMR studies on the structure/function correlations of T-cell-epitope analogs from per
NMR studies on the structure/function correlations of T-cell-epitope analogs from pertussis toxin.
Related Articles NMR studies on the structure/function correlations of T-cell-epitope analogs from pertussis toxin.
Eur J Biochem. 1998 Jun 1;254(2):313-7
Authors: Scarselli M, Esposito G, De Magistris MT, Domenighini M, Rappuoli R, Burroni G, Bernini A, Niccolai N
A synthetic tridecapeptide, corresponding to the 30-42 fragment of the S1 subunit of pertussis toxin, has been structurally characterised by using NMR spectroscopy. The molecule...
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11-17-2010 11:06 PM
[NMR paper] NMR structure of a minimized human agouti related protein prepared by total chemical
NMR structure of a minimized human agouti related protein prepared by total chemical synthesis.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR structure of a minimized human agouti related protein prepared by total chemical synthesis.
FEBS Lett. 1999 May 21;451(2):125-31
Authors: Bolin KA, Anderson DJ, Trulson JA, Thompson DA, Wilken J, Kent SB, Gantz I, Millhauser GL
The structure of the chemically synthesized C-terminal region of the human agouti related protein...
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08-21-2010 04:03 PM
Simultaneous detection of amide and methyl correlations using a time shared NMR experiment: application to binding epitope mapping
Simultaneous detection of amide and methyl correlations using a time shared NMR experiment: application to binding epitope mapping
Peter Würtz, Olli Aitio, Maarit Hellman and Perttu Permi
Journal of Biomolecular NMR; 2007; 39(2) pp 97 - 105
Abstract:
Simultaneous recording of different NMR parameters is an efficient way to reduce the overall experimental time and speed up structural studies of biological macromolecules. This can especially be beneficial in the case of fast NMR-based drug screening applications or for collecting NOE restraints, where prohibitively long data collection...
1H(C) and 1H(N) total NOE correlations in a single 3D NMR experiment. 15N and 13C tim
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