NMR paper 19F-NMR studies of retinol transfer between cellular retinol binding proteins and pho

		BioNMR > Educational resources > Journal club
[NMR paper] 19F-NMR studies of retinol transfer between cellular retinol binding proteins and pho

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-22-2010, 03:31 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,734

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

19F-NMR studies of retinol transfer between cellular retinol binding proteins and pho

19F-NMR studies of retinol transfer between cellular retinol binding proteins and phospholipid vesicles.

Related Articles 19F-NMR studies of retinol transfer between cellular retinol binding proteins and phospholipid vesicles.

FEBS Lett. 1997 Feb 3;402(2-3):116-20

Authors: Rong D, Lin CL, d'Avignon DA, Lovey AJ, Rosenberger M, Li E

The cellular retinol binding proteins, CRBP and CRBP II, are implicated in the cellular uptake of retinol and intracellular trafficking of retinol between sites of metabolic processing. 19F-NMR studies of retinol transfer between CRBP and CRBP II and phospholipid vesicles, using either fluorine-labeled ligand or protein, demonstrated that there was significantly more transfer of retinol from CRBP II to lipid vesicles than from CRBP. Differences in how readily protein-bound retinol is released to lipid bilayers may lead to differences in how these two proteins modulate intracellular retinol metabolism.

PMID: 9037178 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Validation of the binding site structure of the cellular retinol-binding protein (CRB Validation of the binding site structure of the cellular retinol-binding protein (CRBP) by ligand NMR chemical shift perturbations. Related Articles Validation of the binding site structure of the cellular retinol-binding protein (CRBP) by ligand NMR chemical shift perturbations. J Am Chem Soc. 2005 Apr 20;127(15):5310-1 Authors: Wang B, Merz KM We have calculated proton chemical shift perturbations (CSPs) of retinol in the cellular retinol-binding protein (CRBP) through the use of a recently developed computational approach (Wang et al. J....	nmrlearner	Journal club	0	11-25-2010 08:21 PM
[NMR paper] NMR study of the binding of all-trans-retinoic acid to type II human cellular retinoi NMR study of the binding of all-trans-retinoic acid to type II human cellular retinoic acid binding protein. Related Articles NMR study of the binding of all-trans-retinoic acid to type II human cellular retinoic acid binding protein. Biochim Biophys Acta. 1999 Aug 17;1433(1-2):240-52 Authors: Wang L, Yan H Cellular RA binding proteins are thought to play important roles in the (RA), a hormonally active metabolite of vitamin A that has profound effects on cell growth, + differentiation and morphogenesis. Binding of RA to type II human cellular...	nmrlearner	Journal club	0	11-18-2010 08:31 PM
[NMR paper] NMR solution structure of type II human cellular retinoic acid binding protein: impli NMR solution structure of type II human cellular retinoic acid binding protein: implications for ligand binding. Related Articles NMR solution structure of type II human cellular retinoic acid binding protein: implications for ligand binding. Biochemistry. 1998 Sep 15;37(37):12727-36 Authors: Wang L, Li Y, Abildgaard F, Markley JL, Yan H The structure of human apo-cellular retinoic acid binding protein II (apo-CRABPII) in solution at pH 7.3 has been determined by NMR spectroscopy. The sequential assignments of the 1H, 13C, and 15N resonances...	nmrlearner	Journal club	0	11-17-2010 11:15 PM
[NMR paper] Determination of de novo synthesized amino acids in cellular proteins revisited by 13 Determination of de novo synthesized amino acids in cellular proteins revisited by 13C NMR spectroscopy. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_120x27.gif Related Articles Determination of de novo synthesized amino acids in cellular proteins revisited by 13C NMR spectroscopy. NMR Biomed. 1997 Apr;10(2):50-8 Authors: Flögel U, Willker W, Leibfritz D 13C nuclear magnetic resonance spectroscopy was used to determine the absolute amounts to de novo...	nmrlearner	Journal club	0	08-22-2010 03:31 PM
[NMR paper] Determination of de novo synthesized amino acids in cellular proteins revisited by 13 Determination of de novo synthesized amino acids in cellular proteins revisited by 13C NMR spectroscopy. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_120x27.gif Related Articles Determination of de novo synthesized amino acids in cellular proteins revisited by 13C NMR spectroscopy. NMR Biomed. 1997 Apr;10(2):50-8 Authors: Flögel U, Willker W, Leibfritz D 13C nuclear magnetic resonance spectroscopy was used to determine the absolute amounts to de novo...	nmrlearner	Journal club	0	08-22-2010 03:03 PM
[NMR paper] 19F-NMR studies of retinol transfer between cellular retinol binding proteins and pho 19F-NMR studies of retinol transfer between cellular retinol binding proteins and phospholipid vesicles. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles 19F-NMR studies of retinol transfer between cellular retinol binding proteins and phospholipid vesicles. FEBS Lett. 1997 Feb 3;402(2-3):116-20 Authors: Rong D, Lin CL, d'Avignon DA, Lovey AJ, Rosenberger M, Li E The cellular retinol binding proteins, CRBP and CRBP II, are implicated in the cellular uptake of retinol...	nmrlearner	Journal club	0	08-22-2010 03:03 PM
[NMR paper] NMR studies of fluororetinol analogs complexed to two homologous rat cellular retinol NMR studies of fluororetinol analogs complexed to two homologous rat cellular retinol-binding proteins. Related Articles NMR studies of fluororetinol analogs complexed to two homologous rat cellular retinol-binding proteins. Biochim Biophys Acta. 1994 Sep 21;1208(1):136-44 Authors: Rong D, Lovey AJ, Rosenberger M, d'Avignon DA, Li E Comparative 19F-NMR studies of fluororetinol analogs with rat cellular retinol binding protein II (CRBP II) and rat cellular retinol-binding protein (CRBP) were performed to probe differences in the binding...	nmrlearner	Journal club	0	08-22-2010 03:29 AM
[NMR paper] Inter-tryptophan distances in rat cellular retinol binding protein II by solid-state Inter-tryptophan distances in rat cellular retinol binding protein II by solid-state NMR. Related Articles Inter-tryptophan distances in rat cellular retinol binding protein II by solid-state NMR. Biochemistry. 1993 May 4;32(17):4560-3 Authors: McDowell LM, Holl SM, Qian SJ, Li E, Schaefer J Structural constraints for the tryptophans in rat cellular retinol binding protein II (CRBP II) have been obtained by rotational-echo double-resonance (REDOR) solid-state NMR. CRBP II was labeled with L-tryptophan and L-tryptophan. The 13C-19F dipolar...	nmrlearner	Journal club	0	08-21-2010 11:53 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off