NMR paper 19F-NMR studies of retinol transfer between cellular retinol binding proteins and pho

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[NMR paper] 19F-NMR studies of retinol transfer between cellular retinol binding proteins and pho

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NMR processing:

MDD

NMR assignment:

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Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

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TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

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NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

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FANDAS

MestReS

V-NMR

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B-factor

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Shiftx2

Sparta+

Camshift

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ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

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Shifty

Camcoil

Poulsen_rc_CS

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MAXOCC

Format conversion & validation:

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08-22-2010, 03:03 PM

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19F-NMR studies of retinol transfer between cellular retinol binding proteins and pho

19F-NMR studies of retinol transfer between cellular retinol binding proteins and phospholipid vesicles.

Related Articles 19F-NMR studies of retinol transfer between cellular retinol binding proteins and phospholipid vesicles.

FEBS Lett. 1997 Feb 3;402(2-3):116-20

Authors: Rong D, Lin CL, d'Avignon DA, Lovey AJ, Rosenberger M, Li E

The cellular retinol binding proteins, CRBP and CRBP II, are implicated in the cellular uptake of retinol and intracellular trafficking of retinol between sites of metabolic processing. 19F-NMR studies of retinol transfer between CRBP and CRBP II and phospholipid vesicles, using either fluorine-labeled ligand or protein, demonstrated that there was significantly more transfer of retinol from CRBP II to lipid vesicles than from CRBP. Differences in how readily protein-bound retinol is released to lipid bilayers may lead to differences in how these two proteins modulate intracellular retinol metabolism.

PMID: 9037178 [PubMed - indexed for MEDLINE]

Source: PubMed

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