19F NMR Studies of a Desolvated Near-NativeProtein Folding Intermediate

		BioNMR > Educational resources > Journal club
19F NMR Studies of a Desolvated Near-NativeProtein Folding Intermediate

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-16-2013, 08:36 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

19F NMR Studies of a Desolvated Near-NativeProtein Folding Intermediate

19F NMR Studies of a Desolvated Near-NativeProtein Folding Intermediate

Biochemistry
DOI: 10.1021/bi4010057

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] 19F NMR Studies of a Desolvated Near-Native Protein Folding Intermediate. 19F NMR Studies of a Desolvated Near-Native Protein Folding Intermediate. 19F NMR Studies of a Desolvated Near-Native Protein Folding Intermediate. Biochemistry. 2013 Aug 1; Authors: Kitevski-Leblanc JL, Hoang J, Thach W, Larda ST, Prosser RS Abstract While many proteins are recognized to undergo folding via an intermediate, the microscopic nature of folding intermediates is less understood. In this study, 19F NMR and near UV circular dichroism (CD) are used to characterize a transition to a thermal folding intermediate of calmodulin, a...	nmrlearner	Journal club	0	08-04-2013 03:18 AM
Real-Time NMR Characterizationof Structure and Dynamicsin a Transiently Populated Protein Folding Intermediate Real-Time NMR Characterizationof Structure and Dynamicsin a Transiently Populated Protein Folding Intermediate Enrico Rennella, Thomas Cutuil, Paul Schanda, Isabel Ayala, Vincent Forge and Bernhard Brutscher http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja302598j/aop/images/medium/ja-2012-02598j_0006.gif Journal of the American Chemical Society DOI: 10.1021/ja302598j http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/TMtxa4gIZm4	nmrlearner	Journal club	0	05-08-2012 05:37 AM
[NMR paper] A protein folding intermediate of ribonuclease T1 characterized at high resolution by A protein folding intermediate of ribonuclease T1 characterized at high resolution by 1D and 2D real-time NMR spectroscopy. Related Articles A protein folding intermediate of ribonuclease T1 characterized at high resolution by 1D and 2D real-time NMR spectroscopy. J Mol Biol. 1999 Jan 15;285(2):829-42 Authors: Balbach J, Steegborn C, Schindler T, Schmid FX The rate-limiting step during the refolding of S54G/P55N ribonuclease T1 is determined by the slow trans-->cis prolyl isomerisation of Pro39. We investigated the refolding of this variant by...	nmrlearner	Journal club	0	11-18-2010 07:05 PM
Probing the Folding Intermediate of Bacillus subtilis RNase P protein by NMR. Probing the Folding Intermediate of Bacillus subtilis RNase P protein by NMR. Related Articles Probing the Folding Intermediate of Bacillus subtilis RNase P protein by NMR. Biochemistry. 2010 Sep 15; Authors: Chang YC, Franch WR, Oas TG Protein folding intermediates are often imperative to overall folding processes and consequent biological functions. However, the low population and transient nature of the intermediate states often hinder the biochemical and biophysical characterization. Previous studies have demonstrated that Bacillus...	nmrlearner	Journal club	0	09-17-2010 04:14 PM
A Transient and Low-Populated Protein-Folding Intermediate at Atomic Resolution - Sec A Transient and Low-Populated Protein-Folding Intermediate at Atomic Resolution - Securities Industry News (blog) (subscription) <img alt="" height="1" width="1" /> A Transient and Low-Populated Protein-Folding Intermediate at Atomic Resolution Securities Industry News (blog) (subscription) In this work, we used chemical shifts and bond-vector orientation constraints obtained from nuclear magnetic resonance relaxation dispersion spectroscopy, ... Read More	nmrlearner	Online News	0	09-10-2010 12:48 AM
[NMR paper] Detection and characterization of an early folding intermediate of T4 lysozyme using Detection and characterization of an early folding intermediate of T4 lysozyme using pulsed hydrogen exchange and two-dimensional NMR. Related Articles Detection and characterization of an early folding intermediate of T4 lysozyme using pulsed hydrogen exchange and two-dimensional NMR. Biochemistry. 1992 May 26;31(20):4749-56 Authors: Lu J, Dahlquist FW Two-dimensional 1H-15N NMR techniques combined with pulsed hydrogen-deuterium exchange have been used to characterize the folding pathway of T4 lysozyme. In the unfolded state, there is little...	nmrlearner	Journal club	0	08-21-2010 11:41 PM
[NMR paper] Detection and characterization of a folding intermediate in barnase by NMR. Detection and characterization of a folding intermediate in barnase by NMR. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.nature.com-images-lo_nature.gif Related Articles Detection and characterization of a folding intermediate in barnase by NMR. Nature. 1990 Aug 2;346(6283):488-90 Authors: Bycroft M, Matouschek A, Kellis JT, Serrano L, Fersht AR Protein engineering is being developed for mapping the energetics and pathway of protein folding. From kinetic studies on wild-type and mutant proteins, the sequence and energetics of...	nmrlearner	Journal club	0	08-21-2010 11:04 PM
[NMR paper] Real-time NMR studies on a transient folding intermediate of barstar. Real-time NMR studies on a transient folding intermediate of barstar. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Real-time NMR studies on a transient folding intermediate of barstar. Protein Sci. 1999 Jun;8(6):1286-91 Authors: Killick TR, Freund SM, Fersht AR The refolding of barstar, the intracellular...	nmrlearner	Journal club	0	08-21-2010 04:03 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off