19F NMR analysis of the antimicrobial peptide PGLa bound to native cell membranes fro

		BioNMR > Educational resources > Journal club
19F NMR analysis of the antimicrobial peptide PGLa bound to native cell membranes fro

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

10-05-2010, 12:11 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

19F NMR analysis of the antimicrobial peptide PGLa bound to native cell membranes fro

19F NMR analysis of the antimicrobial peptide PGLa bound to native cell membranes from bacterial protoplasts and human erythrocytes.

Related Articles 19F NMR analysis of the antimicrobial peptide PGLa bound to native cell membranes from bacterial protoplasts and human erythrocytes.

J Am Chem Soc. 2010 Jul 7;132(26):8822-4

Authors: Ieronimo M, Afonin S, Koch K, Berditsch M, Wadhwani P, Ulrich AS

(19)F NMR is a unique tool to examine the structure of fluorine-labeled peptides in their native cellular environment, due to an exquisite sensitivity and lack of natural abundance background. For solid-state NMR analysis, we isolated native membranes from erythrocyte ghosts and bacterial protoplasts and prepared them as macroscopically oriented samples. They showed a high purity and quality of alignment according to (31)P NMR, and the membrane-bound antimicrobial peptide PGLa could be detected by (19)F NMR. The characteristic fingerprint splitting of its (19)F reporter group indicated that the peptide helix binds to the native membranes in a surface alignment, albeit with a higher affinity in the prokaryotic than the eukaryotic system.

PMID: 20550126 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Neurotoxin II Bound to Acetylcholine Receptors in Native Membranes Studied by Dynamic Nuclear Polarization NMR Neurotoxin II Bound to Acetylcholine Receptors in Native Membranes Studied by Dynamic Nuclear Polarization NMR Arne H. Linden, Sascha Lange, W. Trent Franks, U?mit Akbey, Edgar Specker, Barth-Jan van Rossum and Hartmut Oschkinat http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja206999c/aop/images/medium/ja-2011-06999c_0003.gif Journal of the American Chemical Society DOI: 10.1021/ja206999c http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/4d1LYPHtCFw	nmrlearner	Journal club	0	11-11-2011 08:26 AM
Probing ground and excited states of phospholamban in model and native lipid membranes by magic angle spinning NMR spectroscopy. Probing ground and excited states of phospholamban in model and native lipid membranes by magic angle spinning NMR spectroscopy. Probing ground and excited states of phospholamban in model and native lipid membranes by magic angle spinning NMR spectroscopy. Biochim Biophys Acta. 2011 Aug 3; Authors: Gustavsson M, Traaseth NJ, Veglia G In this paper, we analyzed the ground and excited states of phospholamban (PLN), a membrane protein that regulates sarcoplasmic reticulum calcium ATPase (SERCA), in different membrane mimetic environments....	nmrlearner	Journal club	0	08-16-2011 01:19 PM
In Situ Structural Characterization of a Recombinant Protein in Native Escherichia coli Membranes with Solid-State Magic-Angle-Spinning NMR In Situ Structural Characterization of a Recombinant Protein in Native Escherichia coli Membranes with Solid-State Magic-Angle-Spinning NMR Riqiang Fu, Xingsheng Wang, Conggang Li, Adriana N. Santiago-Miranda, Gary J. Pielak and Fang Tian http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja204062v/aop/images/medium/ja-2011-04062v_0004.gif Journal of the American Chemical Society DOI: 10.1021/ja204062v http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/BuOPwKpaHdw	nmrlearner	Journal club	0	07-27-2011 11:24 AM
In Situ Structural Characterization of a Recombinant Protein in Native Escherichia coli Membranes with Solid-State MAS NMR. In Situ Structural Characterization of a Recombinant Protein in Native Escherichia coli Membranes with Solid-State MAS NMR. In Situ Structural Characterization of a Recombinant Protein in Native Escherichia coli Membranes with Solid-State MAS NMR. J Am Chem Soc. 2011 Jul 21; Authors: Fu R, Wang X, Li C, Santiago-Miranda AN, Pielak GJ, Tian F The feasibility of using solid state MAS NMR for in situ structural characterization of the LR11 (sorLA) transmembrane domain in native Escherichia coli (E. coli) membranes is presented. LR11 interacts with...	nmrlearner	Journal club	0	07-23-2011 08:54 AM
Solid-State (19)F-NMR of Peptides in Native Membranes. Solid-State (19)F-NMR of Peptides in Native Membranes. Solid-State (19)F-NMR of Peptides in Native Membranes. Top Curr Chem. 2011 May 20; Authors: Koch K, Afonin S, Ieronimo M, Berditsch M, Ulrich AS To understand how membrane-active peptides (MAPs) function in vivo, it is essential to obtain structural information about them in their membrane-bound state. Most biophysical approaches rely on the use of bilayers prepared from synthetic phospholipids, i.e. artificial model membranes. A particularly successful structural method is solid-state NMR,...	nmrlearner	Journal club	0	05-21-2011 07:51 PM
(31)P NMR and AFM studies on the destabilization of cell and model membranes by the major bovine seminal plasma protein, PDC-109. (31)P NMR and AFM studies on the destabilization of cell and model membranes by the major bovine seminal plasma protein, PDC-109. Related Articles (31)P NMR and AFM studies on the destabilization of cell and model membranes by the major bovine seminal plasma protein, PDC-109. IUBMB Life. 2010 Nov;62(11):841-51 Authors: Damai RS, Sankhala RS, Anbazhagan V, Swamy MJ The effect of PDC-109 binding to dimyristoylphosphatidylcholine (DMPC) and dipalmitoylphosphatidylglycerol (DPPG) multilamellar vesicles (MLVs) and supported membranes was investigated by...	nmrlearner	Journal club	0	12-01-2010 04:41 PM
Oligomeric Structure of a Cathelicidin Antimicrobial Peptide in Dodecylphosphocholine Oligomeric Structure of a Cathelicidin Antimicrobial Peptide in Dodecylphosphocholine Micelle Determined by NMR Spectroscopy. Related Articles Oligomeric Structure of a Cathelicidin Antimicrobial Peptide in Dodecylphosphocholine Micelle Determined by NMR Spectroscopy. Biochim Biophys Acta. 2010 Oct 6; Authors: Saravanan R, Bhattacharjya S The broad spectrum of antibacterial activities of host defense cationic antimicrobial peptides (AMPs) arises from their ability to perturb membrane integrity of the microbes. The mechanisms are often thought to...	nmrlearner	Journal club	0	10-12-2010 02:52 PM
Probing membrane topology of the antimicrobial peptide distinctin by solid-state NMR Probing membrane topology of the antimicrobial peptide distinctin by solid-state NMR spectroscopy in zwitterionic and charged lipid bilayers. Related Articles Probing membrane topology of the antimicrobial peptide distinctin by solid-state NMR spectroscopy in zwitterionic and charged lipid bilayers. Biochim Biophys Acta. 2010 Aug 15; Authors: Verardi R, Traaseth NJ, Shi L, Porcelli F, Monfregola L, De Luca S, Amodeo P, Veglia G, Scaloni A Distinctin is a 47-residue antimicrobial peptide, which interacts with negatively charged membranes and is...	nmrlearner	Journal club	0	08-21-2010 01:02 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off