[NMR paper] (19)F Fast Magic-Angle Spinning NMR Spectroscopy on Microcrystalline Complexes of Fluorinated Ligands and the Carbohydrate Recognition Domain of Galectin-3
(19)F Fast Magic-Angle Spinning NMR Spectroscopy on Microcrystalline Complexes of Fluorinated Ligands and the Carbohydrate Recognition Domain of Galectin-3
Structural characterization of protein-ligand binding interfaces at atomic resolution is essential for improving the design of specific and potent inhibitors. Herein, we explored fast ^(19)F- and ¹H-detected magic angle spinning NMR spectroscopy to investigate the interaction between two fluorinated ligand diastereomers with the microcrystalline galectin-3 carbohydrate recognition domain. The detailed environment around the fluorine atoms was mapped by 2D ^(13)C-^(19)F and ¹H-^(19)F dipolar...
[NMR paper] Fast Magic-Angle-Spinning NMR Reveals the Evasive Hepatitis B Virus Capsid*C-Terminal Domain
Fast Magic-Angle-Spinning NMR Reveals the Evasive Hepatitis B Virus Capsid*C-Terminal Domain
Experimentally determined protein structures often feature missing domains. One example is the C terminal domain (CTD) of the hepatitis B virus capsid protein, a functionally central part of this assembly, crucial in regulating nucleic-acid interactions, cellular trafficking, nuclear import, particle assembly and maturation. However, its structure remained elusive to all current techniques, including NMR. Here we show that the recently developed proton-detected fast magic-angle-spinning...
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[NMR paper] Setting the magic angle for fast magic-angle spinning probes
Setting the magic angle for fast magic-angle spinning probes
Publication date: August 2018
Source: Journal of Magnetic Resonance, Volume 293
Author(s): Susanne Penzel, Albert A. Smith, Matthias Ernst, Beat H. Meier
Abstract
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[NMR paper] Setting the magic angle for fast magic-angle spinning probes
Setting the magic angle for fast magic-angle spinning probes
Publication date: Available online 15 June 2018
Source:Journal of Magnetic Resonance</br>
Author(s): Susanne Penzel, Albert A. Smith, Matthias Ernst, Beat H. Meier</br>
Fast magic-angle spinning, coupled with 1H detection is a powerful method to improve spectral resolution and signal to noise in solid-state NMR spectra. Commercial probes now provide spinning frequencies in excess of 100 kHz. Then, one has sufficient resolution in the 1H dimension to directly detect protons, which have a gyromagnetic...
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06-15-2018 07:18 PM
[NMR paper] Chemical Shifts of the Carbohydrate Binding Domain of Galectin-3 from Magic Angle Spinning NMR and Hybrid Quantum Mechanics/Molecular Mechanics Calculations.
Chemical Shifts of the Carbohydrate Binding Domain of Galectin-3 from Magic Angle Spinning NMR and Hybrid Quantum Mechanics/Molecular Mechanics Calculations.
Chemical Shifts of the Carbohydrate Binding Domain of Galectin-3 from Magic Angle Spinning NMR and Hybrid Quantum Mechanics/Molecular Mechanics Calculations.
J Phys Chem B. 2018 Mar 02;:
Authors: Kraus J, Gupta R, Lu M, Yehl JB, Case DA, Gronenborn AM, Akke M, Polenova T
Abstract
Magic angle spinning NMR spectroscopy is uniquely suited to probe the structure and dynamics of...
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03-03-2018 12:01 PM
[NMR paper] Ultra fast magic angle spinning solid - state NMR spectroscopy of intact bone.
Ultra fast magic angle spinning solid - state NMR spectroscopy of intact bone.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-7315-19-Wiley_FullText_120x30_orange.png Related Articles Ultra fast magic angle spinning solid - state NMR spectroscopy of intact bone.
Magn Reson Chem. 2016 Feb;54(2):132-5
Authors: Singh C, Rai RK, Kayastha AM, Sinha N
Abstract
Ultra fast magic angle spinning (MAS) has been a potent method to significantly average out homogeneous/inhomogeneous line...
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01-28-2017 08:29 PM
[NMR paper] Site-Resolved Backbone and Side-Chain Intermediate Dynamics in a Carbohydrate-Binding Module Protein Studied by Magic-Angle Spinning NMR Spectroscopy.
Site-Resolved Backbone and Side-Chain Intermediate Dynamics in a Carbohydrate-Binding Module Protein Studied by Magic-Angle Spinning NMR Spectroscopy.
Site-Resolved Backbone and Side-Chain Intermediate Dynamics in a Carbohydrate-Binding Module Protein Studied by Magic-Angle Spinning NMR Spectroscopy.
Chemistry. 2015 Jun 12;
Authors: Ivanir-Dabora H, Nimerovsky E, Madhu PK, Goldbourt A
Abstract
Magic-angle spinning solid-state NMR spectroscopy has been applied to study the dynamics of CBM3b-Cbh9A from Clostridium thermocellum...
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06-16-2015 07:24 PM
[NMR paper] Site-specific 13C chemical shift anisotropy measurements in a uniformly 15N,13C-labeled microcrystalline protein by 3D magic-angle spinning NMR spectroscopy.
Site-specific 13C chemical shift anisotropy measurements in a uniformly 15N,13C-labeled microcrystalline protein by 3D magic-angle spinning NMR spectroscopy.
Related Articles Site-specific 13C chemical shift anisotropy measurements in a uniformly 15N,13C-labeled microcrystalline protein by 3D magic-angle spinning NMR spectroscopy.
J Am Chem Soc. 2005 Aug 31;127(34):11946-7
Authors: Wylie BJ, Franks WT, Graesser DT, Rienstra CM
In this Communication, we introduce a 3D magic-angle spinning recoupling experiment that correlates chemical shift...
(19)F Fast Magic-Angle Spinning NMR Spectroscopy on Microcrystalline Complexes of Fluorinated Ligands and the Carbohydrate Recognition Domain of Galectin-3
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