NMR paper 13C NMR studies of the binding of medium-chain fatty acids to human serum albumin.

		BioNMR > Educational resources > Journal club
[NMR paper] 13C NMR studies of the binding of medium-chain fatty acids to human serum albumin.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-22-2010, 03:33 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

13C NMR studies of the binding of medium-chain fatty acids to human serum albumin.

13C NMR studies of the binding of medium-chain fatty acids to human serum albumin.

Related Articles 13C NMR studies of the binding of medium-chain fatty acids to human serum albumin.

J Lipid Res. 1994 Mar;35(3):458-67

Authors: Kenyon MA, Hamilton JA

Binding of the medium-chain fatty acids (MCFA), octanoic (OCT) and decanoic (DEC) acid, to human serum albumin (HSA) has been studied by 13C NMR spectroscopy. NMR spectra at 35 degrees C showed an apparently homogeneous binding environment (a single, narrow resonance for the 13C-enriched carboxyl carbon) at different mole ratios and pH values. Changes in the chemical shift of this peak with mole ratio and protein concentration demonstrated rapid equilibration (< or = msec) of bound and unbound MCFA and permitted a direct quantitation of bound/unbound MCFA. Spectra of OCT/HSA mixtures at 6 degrees C revealed at least three distinct binding sites that fill sequentially. The observed heterogeneity of binding at low temperature, compared to 35 degrees C, is attributed to a slower exchange rate of OCT between binding sites. The highest affinity sites for both OCT and DEC have properties similar to those of binding sites for longer-chain fatty acids, such as the close proximity of the fatty acid carboxylate to basic amino acid residue(s). Interestingly, chemical shift data showed that the first mole of OCT and DEC either bind differently to the same site or bind to different sites on HSA. The rapid desorption of MCFA from HSA binding sites has implications for dietary regimens with medium chain triglycerols.

PMID: 8014580 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
DEER in Biological Multispin-Systems: A Case Study on the Fatty Acid Binding to Human Serum Albumin DEER in Biological Multispin-Systems: A Case Study on the Fatty Acid Binding to Human Serum Albumin Publication year: 2011 Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 10 March 2011</br> Matthias J.N., Junk , Hans W., Spiess , Dariush, Hinderberger</br> In this study, self-assembled systems of human serum albumin (HSA) and spin-labeled fatty acids are characterized by double electron–electron resonance (DEER). HSA, being the most important transport protein of the human blood, is capable to host up to seven paramagnetic fatty acid...	nmrlearner	Journal club	0	03-11-2011 05:00 PM
[NMR paper] NMR analysis of synthetic human serum albumin alpha-helix 28 identifies structural di NMR analysis of synthetic human serum albumin alpha-helix 28 identifies structural distortion upon amadori modification. Related Articles NMR analysis of synthetic human serum albumin alpha-helix 28 identifies structural distortion upon amadori modification. J Biol Chem. 2005 Jun 17;280(24):22582-9 Authors: Howard MJ, Smales CM The non-enzymatic reaction between reducing sugars and long-lived proteins in vivo results in the formation of glycation and advanced glycation end products, which alter the properties of proteins including charge,...	nmrlearner	Journal club	0	11-25-2010 08:21 PM
[NMR paper] Analysis of competitive binding of ligands to human serum albumin using NMR relaxatio Analysis of competitive binding of ligands to human serum albumin using NMR relaxation measurements. Related Articles Analysis of competitive binding of ligands to human serum albumin using NMR relaxation measurements. J Pharm Biomed Anal. 2004 Feb 4;34(2):247-54 Authors: Cui YF, Bai GY, Li CG, Ye CH, Liu ML The competitive binding of two ligands, ibuprofen (IBP) and salicylic acid (SAL), to human serum albumin (HSA) was studied by using nuclear magnetic resonance (NMR) relaxation measurements. When the concentration of one ligand was...	nmrlearner	Journal club	0	11-24-2010 09:25 PM
[NMR paper] Interaction of chicken liver basic fatty acid-binding protein with fatty acids: a 13C Interaction of chicken liver basic fatty acid-binding protein with fatty acids: a 13C NMR and fluorescence study. Related Articles Interaction of chicken liver basic fatty acid-binding protein with fatty acids: a 13C NMR and fluorescence study. Biochemistry. 2001 Oct 23;40(42):12604-11 Authors: Beringhelli T, Goldoni L, Capaldi S, Bossi A, Perduca M, Monaco HL Two different groups of liver fatty acid-binding proteins (L-FABPs) are known: the mammalian type and the basic type. Very few members of this second group of L-FABPs have been...	nmrlearner	Journal club	0	11-19-2010 08:44 PM
[NMR paper] Direct binding of ethanol to bovine serum albumin: a fluorescent and 13C NMR multiple Direct binding of ethanol to bovine serum albumin: a fluorescent and 13C NMR multiplet relaxation study. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Direct binding of ethanol to bovine serum albumin: a fluorescent and 13C NMR multiplet relaxation study. Biochemistry. 1996 Jan 9;35(1):340-7 Authors: Avdulov NA, Chochina SV, Daragan VA, Schroeder F, Mayo KH, Wood WG Molecular mechanisms of ethanol interaction with proteins are not well-understood. In the present study, direct...	nmrlearner	Journal club	0	08-22-2010 02:27 PM
[NMR paper] The serum albumin-binding domain of streptococcal protein G is a three-helical bundle The serum albumin-binding domain of streptococcal protein G is a three-helical bundle: a heteronuclear NMR study. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles The serum albumin-binding domain of streptococcal protein G is a three-helical bundle: a heteronuclear NMR study. FEBS Lett. 1996 Jan 8;378(2):190-4 Authors: Kraulis PJ, Jonasson P, Nygren PA, UhlÃ©n M, Jendeberg L, Nilsson B, KÃ¶rdel J Streptococcal protein G (SPG) is a cell surface receptor protein with a...	nmrlearner	Journal club	0	08-22-2010 02:27 PM
[NMR paper] The binding of 5-fluorouracil to native and modified human serum albumin: UV, CD, and The binding of 5-fluorouracil to native and modified human serum albumin: UV, CD, and 1H and 19F NMR investigation. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles The binding of 5-fluorouracil to native and modified human serum albumin: UV, CD, and 1H and 19F NMR investigation. J Pharm Biomed Anal. 1995 Aug;13(9):1087-93 Authors: Bertucci C, Ascoli G, Uccello-Barretta G, Di Bari L, Salvadori P 5-Fluorouracil (FU) is an important and widely used antineoplastic drug...	nmrlearner	Journal club	0	08-22-2010 03:50 AM
[NMR paper] 13C NMR studies of the binding of medium-chain fatty acids to human serum albumin. 13C NMR studies of the binding of medium-chain fatty acids to human serum albumin. Related Articles 13C NMR studies of the binding of medium-chain fatty acids to human serum albumin. J Lipid Res. 1994 Mar;35(3):458-67 Authors: Kenyon MA, Hamilton JA Binding of the medium-chain fatty acids (MCFA), octanoic (OCT) and decanoic (DEC) acid, to human serum albumin (HSA) has been studied by 13C NMR spectroscopy. NMR spectra at 35 degrees C showed an apparently homogeneous binding environment (a single, narrow resonance for the 13C-enriched carboxyl...	nmrlearner	Journal club	0	08-22-2010 03:33 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off