NMR as a Unique Tool in Assessment and Complex Determination of Weak Protein-Protein Interactions.
NMR as a Unique Tool in Assessment and Complex Determination of Weak Protein-Protein Interactions.
NMR as a Unique Tool in Assessment and Complex Determination of Weak Protein-Protein Interactions.
Top Curr Chem. 2011 Aug 2;
Authors: Vinogradova O, Qin J
Protein-protein interactions are crucial for a wide variety of biological processes. These interactions range from high affinity (K (d) < nM) to very low affinity (K (d) > mM). While much is known about the nature of high affinity protein complexes, our knowledge about structural characteristics...
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08-03-2011 12:00 PM
13C-labeled heparan sulfate analogue as a tool to study protein/heparan sulfate interaction by NMR spectroscopy. Application to the CXCL12? chemokine.
13C-labeled heparan sulfate analogue as a tool to study protein/heparan sulfate interaction by NMR spectroscopy. Application to the CXCL12? chemokine.
13C-labeled heparan sulfate analogue as a tool to study protein/heparan sulfate interaction by NMR spectroscopy. Application to the CXCL12? chemokine.
J Am Chem Soc. 2011 Jun 2;
Authors: Laguri C, Sapay N, Simorre JP, Brutscher B, Imberty A, Gans P, Lortat-Jacob H
Heparan sulfate, a polysaccharide of the glycosaminoglycan family characterized by a unique level of complexity, has emerged as a key...
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06-04-2011 11:26 AM
[NMR paper] Spin labels as a tool to identify and characterize protein-ligand interactions by NMR
Spin labels as a tool to identify and characterize protein-ligand interactions by NMR spectroscopy.
Related Articles Spin labels as a tool to identify and characterize protein-ligand interactions by NMR spectroscopy.
Chembiochem. 2002 Mar 1;3(2-3):167-73
Authors: Jahnke W
NMR spectroscopy based discovery and optimization of lead compounds for a given molecular target requires the development of methods with maximum sensitivity and robustness. It is shown here that organic nitroxide radicals ("spin labels") can be used to boost the sensitivity...
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11-24-2010 08:49 PM
[NMR paper] NMR structure of human apolipoprotein C-II in the presence of sodium dodecyl sulfate.
NMR structure of human apolipoprotein C-II in the presence of sodium dodecyl sulfate.
Related Articles NMR structure of human apolipoprotein C-II in the presence of sodium dodecyl sulfate.
Biochemistry. 2001 May 8;40(18):5414-21
Authors: MacRaild CA, Hatters DM, Howlett GJ, Gooley PR
The structure and protein-detergent interactions of apolipoprotein C-II (apoC-II) in the presence of SDS micelles have been investigated using circular dichroism and heteronuclear NMR techniques applied to (15)N-labeled protein. Micellar SDS, a commonly used...
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11-19-2010 08:32 PM
[NMR paper] Porcine cerebroside sulfate activator (saposin B) secondary structure: CD, FTIR, and
Porcine cerebroside sulfate activator (saposin B) secondary structure: CD, FTIR, and NMR studies.
Related Articles Porcine cerebroside sulfate activator (saposin B) secondary structure: CD, FTIR, and NMR studies.
Mol Genet Metab. 1998 Jan;63(1):14-25
Authors: Waring AJ, Chen Y, Faull KF, Stevens R, Sherman MA, Fluharty AL
Cerebroside sulfate activator protein (CSAct or saposin B) is one of a group of heat stable, low-molecular-weight proteins that appear to share a common structural motif. These have been referred to as saposin-like proteins...
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11-17-2010 11:06 PM
[NMR paper] Partial purification and substrate specificity of heparan sulfate alpha-N-acetylgluco
Partial purification and substrate specificity of heparan sulfate alpha-N-acetylglucosaminyltransferase I: synthesis, NMR spectroscopic characterization and in vitro assays of two aryl tetrasaccharides.
Related Articles Partial purification and substrate specificity of heparan sulfate alpha-N-acetylglucosaminyltransferase I: synthesis, NMR spectroscopic characterization and in vitro assays of two aryl tetrasaccharides.
Glycobiology. 1997 Jul;7(5):587-95
Authors: Fritz TA, Agrawal PK, Esko JD, Krishna NR
Studies of heparan sulfate biosynthesis...
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08-22-2010 03:31 PM
[NMR paper] Partial purification and substrate specificity of heparan sulfate alpha-N-acetylgluco
Partial purification and substrate specificity of heparan sulfate alpha-N-acetylglucosaminyltransferase I: synthesis, NMR spectroscopic characterization and in vitro assays of two aryl tetrasaccharides.
Related Articles Partial purification and substrate specificity of heparan sulfate alpha-N-acetylglucosaminyltransferase I: synthesis, NMR spectroscopic characterization and in vitro assays of two aryl tetrasaccharides.
Glycobiology. 1997 Jul;7(5):587-95
Authors: Fritz TA, Agrawal PK, Esko JD, Krishna NR
Studies of heparan sulfate biosynthesis...
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08-22-2010 03:03 PM
[NMR paper] Location of M13 coat protein in sodium dodecyl sulfate micelles as determined by NMR.
Location of M13 coat protein in sodium dodecyl sulfate micelles as determined by NMR.
Related Articles Location of M13 coat protein in sodium dodecyl sulfate micelles as determined by NMR.
Biochemistry. 1994 Nov 8;33(44):12990-7
Authors: Papavoine CH, Konings RN, Hilbers CW, van de Ven FJ
The major coat protein (gVIIIp) of bacteriophage M13 solubilized in sodium dodecyl sulfate (SDS) detergent micelles was used as a model system to study this protein in the lipid-bound form. In order to probe the position of gVIIIp relative to the SDS...
13C-Labeled Heparan Sulfate Analogue as a Tool To Study Protein/Heparan Sulfate Interactions by NMR Spectroscopy: Application to the CXCL12? Chemokine
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