13C direct-detection biomolecular NMR spectroscopy in living cells.

		BioNMR > Educational resources > Journal club
13C direct-detection biomolecular NMR spectroscopy in living cells.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

06-18-2011, 01:10 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

13C direct-detection biomolecular NMR spectroscopy in living cells.

13C direct-detection biomolecular NMR spectroscopy in living cells.

13C direct-detection biomolecular NMR spectroscopy in living cells.

Angew Chem Int Ed Engl. 2011 Mar 1;50(10):2339-41

Authors: Bertini I, Felli IC, Gonnelli L, Kumar M V V, Pierattelli R

PMID: 21351349 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Speeding up direct 15N detection: hCaN 2D NMR experiment Speeding up direct 15N detection: hCaN 2D NMR experiment Abstract Experiments detecting low gyromagnetic nuclei have recently been proposed to utilize the relatively slow relaxation properties of these nuclei in comparison to 1H. Here we present a new type of 15N direct-detection experiment. Like the previously proposed CaN experiment (Takeuchi et al. in J Biomol NMR 47:271â??282, 2010), the hCaN experiment described here sequentially connects amide 15N resonances, but utilizes the initial high polarization and the faster recovery of the 1H nucleus to shorten the recycling delay. This...	nmrlearner	Journal club	0	11-14-2011 08:45 AM
STD and trNOESY NMR study of receptor-ligand interactions in living cancer cells. STD and trNOESY NMR study of receptor-ligand interactions in living cancer cells. STD and trNOESY NMR study of receptor-ligand interactions in living cancer cells. Chembiochem. 2011 Mar 21;12(5):695-9 Authors: Potenza D, Vasile F, Belvisi L, Civera M, Araldi EM	nmrlearner	Journal club	0	07-19-2011 07:52 PM
Direct Detection of Nitrogen-14 in Solid-State NMR Spectroscopy Direct Detection of Nitrogen-14 in Solid-State NMR Spectroscopy Publication year: 2011 Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 20 April 2011</br> Luke A., O’Dell</br> Highlights:? Experimental methods for the direct detection of 14N are surveyed ? Advantages, disadvantages and practicalities of each technique are discussed ? Includes single-crystal, ultra-wideline, MAS and overtone spectroscopy</br></br> More...	nmrlearner	Journal club	0	04-21-2011 03:00 PM
[Question from NMRWiki Q&A forum] How to set up 15N direct detection on UnityInova? How to set up 15N direct detection on UnityInova? Has anyone recorded directly detected 15N spectra on UnityInova? We have a triple resonance HCN probe. The sample I've used was natural abundance 1M NaNO3 - sodium nitrate. I've tried to set up cable analogously to 13C detection - through the second preamp, but could not see any signal at all. Check if somebody has answered this question on NMRWiki QA forum	nmrlearner	News from other NMR forums	0	08-22-2010 02:30 AM
[Question from NMRWiki Q&A forum] What has been done with direct detection of Deuterium? What has been done with direct detection of Deuterium? Hello, I am trying to investigate what has been done in NMR using direct detection of deuterium in liquids. - Could you suggest any citations? What kind of sensitivity gain there is in using broadband probe vs. lock channel? Has anything been done/can be done to detect scalar couplings with protons like in COSY or TOCSY? Are HD couplings other than geminal detectable? Thanks.	nmrlearner	News from other NMR forums	0	08-22-2010 02:30 AM
[NMR paper] Direct observation of cell wall structure in living plant tissues by solid-state C NM Direct observation of cell wall structure in living plant tissues by solid-state C NMR spectroscopy. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Direct observation of cell wall structure in living plant tissues by solid-state C NMR spectroscopy. Plant Physiol. 1990 Jan;92(1):61-5 Authors: Jarvis MC, Apperley DC Solid-state (13)C nuclear magnetic resonance (NMR) spectra of the following intact plant tissues were recorded by the crosspolarization...	nmrlearner	Journal club	0	08-21-2010 10:48 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off