Author(s): Pavlo Bielytskyi, Daniel Gräsing, Kaustubh R. Mote, Karthick Babu Sai Sankar Gupta, Shimon Vega, P.K. Madhu, A. Alia, Jörg Matysik
Abstract
In the present study, we exploit the light-induced hyperpolarization occurring on 13C nuclei due to the solid-state photochemically induced dynamic nuclear polarization (photo-CIDNP) effect to boost the NMR signal intensity of selected protons via inverse cross-polarization. Such hyperpolarization transfer is implemented into 1H-detected two-dimensional 13C–1H correlation magic-angle-spinning (MAS) NMR experiment to study protons in frozen photosynthetic reaction centers (RCs). As a first trial, the performance of such an experiment is tested on selectively 13C labeled RCs from the purple bacteria of Rhodobacter sphaeroides. We observed response from the protons belonging to the photochemically active cofactors in their native protein environment. Such an approach is a potential heteronuclear spin-torch experiment which could be complementary to the classical heteronuclear correlation (HETCOR) experiments for mapping proton chemical shifts of photosynthetic cofactors and to understand the role of the proton pool around the electron donors in the electron transfer process occurring during photosynthesis.
[NMR paper] 13C->1H transfer of light-induced hyperpolarization allows for selective detection of protons in frozen photosynthetic reaction center
13C->1H transfer of light-induced hyperpolarization allows for selective detection of protons in frozen photosynthetic reaction center
Publication date: Available online 8 June 2018
Source:Journal of Magnetic Resonance</br>
Author(s): Pavlo Bielytskyi, Daniel Gräsing, Kaustubh R. Mote, Karthick Babu Sai Sankar Gupta, Shimon Vega, P.K. Madhu, A. Alia, Jörg Matysik</br>
In the present study, we exploit the light-induced hyperpolarization occurring on 13C nuclei due to the solid-state photochemically induced dynamic nuclear polarization (photo-CIDNP)...
Hyperpolarization of Frozen Hydrocarbon Gases by Dynamic Nuclear Polarization at 1.2 K #DNPNMR
From The DNP-NMR Blog:
Hyperpolarization of Frozen Hydrocarbon Gases by Dynamic Nuclear Polarization at 1.2 K #DNPNMR
Vuichoud, B., et al., Hyperpolarization of Frozen Hydrocarbon Gases by Dynamic Nuclear Polarization at 1.2 K. J Phys Chem Lett, 2016. 7(16): p. 3235-9.
https://www.ncbi.nlm.nih.gov/pubmed/27483034
[NMR paper] Photochemically Induced Dynamic Nuclear Polarization Observed by Solid-state NMR in a Uniformly (13)C-isotope Labeled Photosynthetic Reaction Center.
Photochemically Induced Dynamic Nuclear Polarization Observed by Solid-state NMR in a Uniformly (13)C-isotope Labeled Photosynthetic Reaction Center.
Related Articles Photochemically Induced Dynamic Nuclear Polarization Observed by Solid-state NMR in a Uniformly (13)C-isotope Labeled Photosynthetic Reaction Center.
J Phys Chem B. 2015 Jun 25;
Authors: Paul S, Bode BE, Matysik J, Alia A
Abstract
A sample of solubilized and quinone-depleted reaction centers (RC) from the purple bacterium Rhodobacter (R.) sphaeroides wild-type (WT)...
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06-26-2015 09:55 PM
[NMR paper] Transfer Rate Edited Experiment for the Selective Detection of Chemical Exchange via Saturaion Transfer (TRE-CEST)
Transfer Rate Edited Experiment for the Selective Detection of Chemical Exchange via Saturaion Transfer (TRE-CEST)
Publication date: Available online 7 May 2015
Source:Journal of Magnetic Resonance</br>
Author(s): Joshua I. Friedman , Ding Xia , Ravinder R. Regatte , Alexej Jerschow</br>
Chemical Exchange Saturation Transfer (CEST) magnetic resonance experiments have become valuable tools in magnetic resonance for the detection of low concentration solutes with far greater sensitivity than direct detection methods. Accurate measures of rates of chemical exchange...
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05-10-2015 07:49 PM
Solid-state NMR applied to photosynthetic light-harvesting complexes.
Solid-state NMR applied to photosynthetic light-harvesting complexes.
Solid-state NMR applied to photosynthetic light-harvesting complexes.
Photosynth Res. 2011 Aug 13;
Authors: Pandit A, de Groot HJ
This short review describes how solid-state NMR has provided a mechanistic and electronic picture of pigment-protein and pigment-pigment interactions in photosynthetic antenna complexes. NMR results on purple bacterial antenna complexes show how the packing of the protein and the pigments inside the light-harvesting oligomers induces mutual...
13C -> 1H transfer of light-induced hyperpolarization allows for selective detection of protons in frozen photosynthetic reaction center
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