(1)H NMR-Based Metabolite Profiling of Plasma in a Rat Model of Chronic Kidney Disease.
PLoS One. 2014 Jan 20;9(1):e85445
Authors: Kim JA, Choi HJ, Kwon YK, Ryu do H, Kwon TH, Hwang GS
Abstract
Chronic kidney disease (CKD) is characterized by the gradual loss of the kidney function to excrete wastes and fluids from the blood. (1)H NMR-based metabolomics was exploited to investigate the altered metabolic pattern in rats with CKD induced by surgical reduction of the renal mass (i.e., 5/6 nephrectomy (5/6 Nx)), particularly for identifying specific metabolic biomarkers associated with early of CKD. Plasma metabolite profiling was performed in CKD rats (at 4- or 8-weeks after 5/6 Nx) compared to sham-operated rats. Principle components analysis (PCA), partial least squares-discriminant analysis (PLS-DA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) score plots showed a significant separation between the groups. The resulting metabolic profiles demonstrated significantly increased plasma levels of organic anions, including citrate, ?-hydroxybutyrate, lactate, acetate, acetoacetate, and formate in CKD. Moreover, levels of alanine, glutamine, and glutamate were significantly higher. These changes were likely to be associated with complicated metabolic acidosis in CKD for counteracting systemic metabolic acidosis or increased protein catabolism from muscle. In contrast, levels of VLDL/LDL (CH2)n and N-acetylglycoproteins were decreased. Taken together, the observed changes of plasma metabolite profiles in CKD rats provide insights into the disturbed metabolism in early phase of CKD, in particular for the altered metabolism of acid-base and/or amino acids.
[NMR paper] Metabonomic profiling of serum and urine by (1)H NMR-based spectroscopy discriminates patients with chronic obstructive pulmonary disease and healthy individuals.
Metabonomic profiling of serum and urine by (1)H NMR-based spectroscopy discriminates patients with chronic obstructive pulmonary disease and healthy individuals.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.plosone.org-images-pone_120x30.png http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Metabonomic profiling of serum and urine by (1)H NMR-based spectroscopy discriminates patients with chronic obstructive pulmonary disease and healthy...
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01-15-2014 05:16 PM
[NMR paper] 1H NMR Based Targeted Metabolite Profiling for Understanding the Complex Relationship Connecting Oxidative Stress with Endometriosis.
1H NMR Based Targeted Metabolite Profiling for Understanding the Complex Relationship Connecting Oxidative Stress with Endometriosis.
1H NMR Based Targeted Metabolite Profiling for Understanding the Complex Relationship Connecting Oxidative Stress with Endometriosis.
Biomed Res Int. 2013;2013:329058
Authors: Jana SK, Dutta M, Joshi M, Srivastava S, Chakravarty B, Chaudhury K
Abstract
Accumulating evidence indicates the active role of oxidative stress in the development of endometriosis; however, the mechanism of reactive oxygen species...
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08-29-2013 01:53 PM
[NMR paper] Comparison of GC-MS and NMR for metabolite profiling of rice subjected to submergence stress.
Comparison of GC-MS and NMR for metabolite profiling of rice subjected to submergence stress.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Comparison of GC-MS and NMR for metabolite profiling of rice subjected to submergence stress.
J Proteome Res. 2013 Feb 1;12(2):898-909
Authors: Barding GA, Béni S, Fukao T, Bailey-Serres J, Larive CK
Abstract
Natural disasters such as drought, extreme temperatures, and flooding can severely impact crop production....
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07-06-2013 09:57 PM
[NMR paper] NMR-based metabolomics of urine in a mouse model of Alzheimer's disease: identification of oxidative stress biomarkers.
NMR-based metabolomics of urine in a mouse model of Alzheimer's disease: identification of oxidative stress biomarkers.
Related Articles NMR-based metabolomics of urine in a mouse model of Alzheimer's disease: identification of oxidative stress biomarkers.
J Clin Biochem Nutr. 2013 Mar;52(2):133-8
Authors: Fukuhara K, Ohno A, Ota Y, Senoo Y, Maekawa K, Okuda H, Kurihara M, Okuno A, Niida S, Saito Y, Takikawa O
Abstract
Alzheimer's disease (AD) is the most common cause of neurodegenerative dementia among elderly patients. A biomarker...
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03-26-2013 01:30 PM
Nuclear magnetic resonance (NMR)-based drug metabolite profiling.
Nuclear magnetic resonance (NMR)-based drug metabolite profiling.
Nuclear magnetic resonance (NMR)-based drug metabolite profiling.
Methods Mol Biol. 2011;708:299-319
Authors: Lenz EM
The identification of drug metabolites in biofluids such as urine, plasma and bile is an important step in drug discovery and development. Proton nuclear magnetic resonance ((1)H-NMR) spectroscopy can provide detailed information regarding the structural transformation of a compound as a consequence of metabolism. However, successful identification of drug...
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04-09-2011 10:57 AM
Detection of autosomal dominant polycystic kidney disease by NMR spectroscopic fingerprinting of urine.
Detection of autosomal dominant polycystic kidney disease by NMR spectroscopic fingerprinting of urine.
Detection of autosomal dominant polycystic kidney disease by NMR spectroscopic fingerprinting of urine.
Kidney Int. 2011 Mar 9;
Authors: Gronwald W, Klein MS, Zeltner R, Schulze BD, Reinhold SW, Deutschmann M, Immervoll AK, Böger CA, Banas B, Eckardt KU, Oefner PJ
Autosomal dominant polycystic kidney disease (ADPKD) is a frequent cause of kidney failure; however, urinary biomarkers for the disease are lacking. In a step towards identifying such...
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03-11-2011 03:14 PM
1H NMR-based metabolic profiling reveals inherent biological variation in yeast and nematode model systems
1H NMR-based metabolic profiling reveals inherent biological variation in yeast and nematode model systems
Abstract The application of metabolomics to human and animal model systems is poised to provide great insight into our understanding of disease etiology and the metabolic changes that are associated with these conditions. However, metabolomic studies have also revealed that there is significant, inherent biological variation in human samples and even in samples from animal model systems where the animals are housed under carefully controlled conditions. This inherent biological...
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03-03-2011 02:06 AM
Nobel Laureate Presents Chronic Wasting Disease Lecture at UW - University of Wyoming
Nobel Laureate Presents Chronic Wasting Disease Lecture at UW - University of Wyoming News
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University of Wyoming News
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Nobel Laureate Presents Chronic Wasting Disease Lecture at UW
University of Wyoming News
His research interests include molecular structural biology, protein science and structural genomics with an emphasis on using nuclear magnetic resonance ...