Research and Markets: Protein-Ligand Interactions. Methods and Principles in ... - Business Wire (press release)
Research and Markets: Protein-Ligand Interactions. Methods and Principles in ... - Business Wire (press release)
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Research and Markets: Protein-Ligand Interactions. Methods and Principles in ...
Business Wire (press release)
The first part provides a basic understanding of the factors governing protein-ligand interaction, followed by a comparison of the four key experimental methods (calorimetry, surface plasmon resonance, NMR and X-ray crystallography) used in generating ...
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08-29-2012 04:31 PM
[Question from NMRWiki Q&A forum] Observing protein-ligand interaction using weakly soluble compounds
Observing protein-ligand interaction using weakly soluble compounds
I am trying to setup an experiment using HSQC with titration of a compound to determine the Kd of the protein-ligand interaction. My difficulties have been in the sample preparation/formulation, as these novel compounds are very weakly soluble in aqueous buffers. I must be able to quantify the soluble concentration of compound over ~6-8 points to generate the binding curve (signal intensity/chemical shift vs. ).
Previous observation of this protein by HSQC and other NMR techniques have used a buffer containing 5 mM...
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07-31-2012 08:27 PM
Automated NMR Resonance Assignment of Large Proteins for Protein-Ligand Interaction Studies.
Automated NMR Resonance Assignment of Large Proteins for Protein-Ligand Interaction Studies.
Automated NMR Resonance Assignment of Large Proteins for Protein-Ligand Interaction Studies.
J Am Chem Soc. 2010 Dec 16;
Authors: Gossert AD, Hiller S, Ferna?ndez C
The detection and structural characterization of protein-ligand interactions by solution NMR is central to functional biology research as well as to drug discovery. Here we present a robust and highly automated procedure for obtaining the resonance assignments necessary for studies of such...
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12-18-2010 12:00 PM
Automated NMR Resonance Assignment of Large Proteins for Protein-Ligand Interaction Studies
Automated NMR Resonance Assignment of Large Proteins for Protein-Ligand Interaction Studies
Alvar D. Gossert, Sebastian Hiller and Ce?sar Ferna?ndez
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja108383x/aop/images/medium/ja-2010-08383x_0004.gif
Journal of the American Chemical Society
DOI: 10.1021/ja108383x
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/E3PMYeBSCeE
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12-17-2010 12:50 AM
[NMR paper] NMR and MD studies on the interaction between ligand peptides and alpha-bungarotoxin.
NMR and MD studies on the interaction between ligand peptides and alpha-bungarotoxin.
Related Articles NMR and MD studies on the interaction between ligand peptides and alpha-bungarotoxin.
J Mol Biol. 2004 Jun 18;339(5):1169-77
Authors: Bernini A, Ciutti A, Spiga O, Scarselli M, Klein S, Vannetti S, Bracci L, Lozzi L, Lelli B, Falciani C, Neri P, Niccolai N
The interaction between alpha-bungarotoxin and linear synthetic peptides, mimotope of the nicotinic acetylcholine receptor binding site, has been characterised extensively by several...
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11-24-2010 09:51 PM
[NMR paper] NMR methods for the determination of protein-ligand dissociation constants.
NMR methods for the determination of protein-ligand dissociation constants.
Related Articles NMR methods for the determination of protein-ligand dissociation constants.
Curr Top Med Chem. 2003;3(1):39-53
Authors: Fielding L
This article is a review with 83 references of the application of NMR to the measurement of the dissociation constants of protein-ligand complexes. After briefly discussing some general concepts of molecular stability, the text turns to consider which NMR parameters are reporters of complex formation. The available data...
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11-24-2010 09:01 PM
[NMR paper] Carbohydrate-protein interaction studies by laser photo CIDNP NMR methods.
Carbohydrate-protein interaction studies by laser photo CIDNP NMR methods.
Related Articles Carbohydrate-protein interaction studies by laser photo CIDNP NMR methods.
Glycoconj J. 1997 Jun;14(4):531-4
Authors: Siebert HC, Kaptein R, Beintema JJ, Soedjanaatmadja UM, Wright CS, Rice A, Kleineidam RG, Kruse S, Schauer R, Pouwels PJ, Kamerling JP, Gabius HJ, Vliegenthart JF
The side chains of tyrosine, tryptophan and histidine are able to produce CIDNP (Chemically Induced Dynamic Nuclear Polarization) signals after laser irradiation in the...
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08-22-2010 03:31 PM
[NMR paper] Carbohydrate-protein interaction studies by laser photo CIDNP NMR methods.
Carbohydrate-protein interaction studies by laser photo CIDNP NMR methods.
Related Articles Carbohydrate-protein interaction studies by laser photo CIDNP NMR methods.
Glycoconj J. 1997 Jun;14(4):531-4
Authors: Siebert HC, Kaptein R, Beintema JJ, Soedjanaatmadja UM, Wright CS, Rice A, Kleineidam RG, Kruse S, Schauer R, Pouwels PJ, Kamerling JP, Gabius HJ, Vliegenthart JF
The side chains of tyrosine, tryptophan and histidine are able to produce CIDNP (Chemically Induced Dynamic Nuclear Polarization) signals after laser irradiation in the...