[ASAP] Studying Homo-oligomerization and Hetero-oligomerization of MDMX and MDM2 Proteins in Single Living Cells by Using In Situ Fluorescence Correlation Spectroscopy

		BioNMR > Educational resources > Journal club
[ASAP] Studying Homo-oligomerization and Hetero-oligomerization of MDMX and MDM2 Proteins in Single Living Cells by Using In Situ Fluorescence Correlation Spectroscopy

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

04-20-2021, 12:46 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,734

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

[ASAP] Studying Homo-oligomerization and Hetero-oligomerization of MDMX and MDM2 Proteins in Single Living Cells by Using In Situ Fluorescence Correlation Spectroscopy

[ASAP] Studying Homo-oligomerization and Hetero-oligomerization of MDMX and MDM2 Proteins in Single Living Cells by Using In Situ Fluorescence Correlation Spectroscopy

Biochemistry
DOI: 10.1021/acs.biochem.1c00153

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
In-situ measurement of 3-D protein structure inside living eukaryotic cells - Phys.org In-situ measurement of 3-D protein structure inside living eukaryotic cells - Phys.org In-situ measurement of 3-D protein structure inside living eukaryotic cells Phys.org Read here	nmrlearner	Online News	0	08-15-2020 05:51 AM
In-situ measurement of 3D protein structure inside living eukaryotic cells - Science Daily In-situ measurement of 3D protein structure inside living eukaryotic cells - Science Daily In-situ measurement of 3D protein structure inside living eukaryotic cells Science Daily Read here	nmrlearner	Online News	0	02-29-2020 09:52 PM
[ASAP] Single-Molecule Tracking Approaches to Protein Synthesis Kinetics in Living Cells Single-Molecule Tracking Approaches to Protein Synthesis Kinetics in Living Cells https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.8b00917/20181114/images/medium/bi-2018-009172_0004.gif Biochemistry DOI: 10.1021/acs.biochem.8b00917 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/teMgPiYQoO4 More...	nmrlearner	Journal club	0	11-25-2018 06:02 AM
[ASAP] Charged Residues in the C-Terminal Domain of Apolipoprotein A-I Modulate Oligomerization Charged Residues in the C-Terminal Domain of Apolipoprotein A-I Modulate Oligomerization https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b01052/20180403/images/medium/bi-2017-01052v_0008.gif Biochemistry DOI: 10.1021/acs.biochem.7b01052 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/y5DDiSZBMb4 More...	nmrlearner	Journal club	0	04-03-2018 08:27 PM
Quantitative Study of the Oligomerization of YeastPrion Sup35NM Proteins Quantitative Study of the Oligomerization of YeastPrion Sup35NM Proteins http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00966/20171204/images/medium/bi-2017-00966w_0013.gif Biochemistry DOI: 10.1021/acs.biochem.7b00966 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/YZOxEWWpbhQ More...	nmrlearner	Journal club	0	12-05-2017 07:35 PM
[NMR paper] Oligomerization of the antimicrobial peptide Protegrin-5 in a membrane-mimicking environment. Structural studies by high-resolution NMR spectroscopy. Oligomerization of the antimicrobial peptide Protegrin-5 in a membrane-mimicking environment. Structural studies by high-resolution NMR spectroscopy. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Oligomerization of the antimicrobial peptide Protegrin-5 in a membrane-mimicking environment. Structural studies by high-resolution NMR spectroscopy. Eur Biophys J. 2017 Apr;46(3):293-300 Authors: Usachev KS, Kolosova OA, Klochkova EA, Yulmetov AR,...	nmrlearner	Journal club	0	03-21-2017 11:26 PM
[NMR paper] A new carbamidemethyl-linked lanthanoid chelating tag for PCS NMR spectroscopy of proteins in living HeLa cells. A new carbamidemethyl-linked lanthanoid chelating tag for PCS NMR spectroscopy of proteins in living HeLa cells. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles A new carbamidemethyl-linked lanthanoid chelating tag for PCS NMR spectroscopy of proteins in living HeLa cells. J Biomol NMR. 2016 Sep 8; Authors: Hikone Y, Hirai G, Mishima M, Inomata K, Ikeya T, Arai S, Shirakawa M, Sodeoka M, Ito Y Abstract Structural analyses of...	nmrlearner	Journal club	0	09-22-2016 06:31 AM
[NMR paper] Non-invasive Analysis of Proteins in Living Cells Using NMR Spectroscopy. Non-invasive Analysis of Proteins in Living Cells Using NMR Spectroscopy. Non-invasive Analysis of Proteins in Living Cells Using NMR Spectroscopy. Yakugaku Zasshi. 2015;135(3):391-8 Authors: Tochio H, Murayama S, Inomata K, Morimoto D, Ohno A, Shirakawa M Abstract **NMR spectroscopy enables structural analyses of proteins and has been widely used in the structural biology field in recent decades. NMR spectroscopy can be applied to proteins inside living cells, allowing characterization of their structures and dynamics in...	nmrlearner	Journal club	0	03-12-2015 10:33 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off