[ASAP] Structural, Dynamic, and Functional Characterization of a DnaX Mini-intein Derived from Spirulina platensis Provides Important Insights into Intein-Mediated Catalysis of Protein Splicing
[ASAP] Structural, Dynamic, and Functional Characterization of a DnaX Mini-intein Derived from Spirulina platensis Provides Important Insights into Intein-Mediated Catalysis of Protein Splicing
[ASAP] Identification and Characterization of a Minimal Functional Splicing Regulatory Protein, PTBP1
Identification and Characterization of a Minimal Functional Splicing Regulatory Protein, PTBP1
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.0c00664/20201207/images/medium/bi0c00664_0006.gif
Biochemistry
DOI: 10.1021/acs.biochem.0c00664
http://feeds.feedburner.com/~r/acs/bichaw/~4/_CVvGWcVZ0Q
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12-08-2020 01:36 PM
[ASAP] Protein Splicing Activity of the Haloferax volcanii PolB-c Intein Is Sensitive to Homing Endonuclease Domain Mutations
Protein Splicing Activity of the Haloferax volcanii PolB-c Intein Is Sensitive to Homing Endonuclease Domain Mutations
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.0c00512/20200902/images/medium/bi0c00512_0007.gif
Biochemistry
DOI: 10.1021/acs.biochem.0c00512
http://feeds.feedburner.com/~r/acs/bichaw/~4/mpEhtA77PW8
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09-13-2020 09:18 AM
[ASAP] Allosteric Influence of Extremophile Hairpin Motif Mutations on the Protein Splicing Activity of a Hyperthermophilic Intein
Allosteric Influence of Extremophile Hairpin Motif Mutations on the Protein Splicing Activity of a Hyperthermophilic Intein
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.0c00348/20200624/images/medium/bi0c00348_0008.gif
Biochemistry
DOI: 10.1021/acs.biochem.0c00348
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06-25-2020 02:58 AM
[NMR paper] NMR Structure and Dynamics of TonB Investigated by Scar-Less Segmental Isotopic Labeling Using a Salt-Inducible Split Intein.
NMR Structure and Dynamics of TonB Investigated by Scar-Less Segmental Isotopic Labeling Using a Salt-Inducible Split Intein.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--www.frontiersin.org-alerts-logo-Logo_LinkOut.jpg Related Articles NMR Structure and Dynamics of TonB Investigated by Scar-Less Segmental Isotopic Labeling Using a Salt-Inducible Split Intein.
Front Chem. 2020;8:136
Authors: Ciragan A, Backlund SM, Mikula KM, Beyer HM, Samuli Ollila OH, Iwaï H
Abstract
The growing understanding of...
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04-09-2020 07:27 PM
[NMR paper] Solution NMR structure of CsgE: Structural insights into a chaperone and regulator protein important for functional amyloid formation.
Solution NMR structure of CsgE: Structural insights into a chaperone and regulator protein important for functional amyloid formation.
Related Articles Solution NMR structure of CsgE: Structural insights into a chaperone and regulator protein important for functional amyloid formation.
Proc Natl Acad Sci U S A. 2016 Jun 13;
Authors: Shu Q, Krezel AM, Cusumano ZT, Pinkner JS, Klein R, Hultgren SJ, Frieden C
Abstract
Curli, consisting primarily of major structural subunit CsgA, are functional amyloids produced on the surface of...
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06-15-2016 11:12 PM
[NMR paper] A Streamlined Method for Preparing Split Intein for NMR Study.
A Streamlined Method for Preparing Split Intein for NMR Study.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles A Streamlined Method for Preparing Split Intein for NMR Study.
Protein Expr Purif. 2014 Apr 18;
Authors: Lee YZ, Lee YT, Lin YJ, Chen YJ, Sue SC
Abstract
A protein ligase, intein, mediates a protein-splicing reaction. It can be split into two complementary fragments and reconstituted as a whole intein scaffold to perform protein...
nmrlearner
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04-23-2014 06:31 PM
(1)H, (13)C, and (15)N NMR assignments of the Pyrococcus abyssi DNA polymerase II intein.
(1)H, (13)C, and (15)N NMR assignments of the Pyrococcus abyssi DNA polymerase II intein.
(1)H, (13)C, and (15)N NMR assignments of the Pyrococcus abyssi DNA polymerase II intein.
Biomol NMR Assign. 2011 Apr 26;
Authors: Liu J, Du Z, Albracht CD, Naidu RO, Mills KV, Wang C
Protein splicing is a precise post-translational process mediated by inteins. Inteins are intervening proteins that cleave themselves from a precursor protein while joining the flanking sequences. Here we report the (15)N, (13)C, and (1)H chemical shift assignments of the intein...
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04-27-2011 04:03 PM
[NMR paper] Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically l
Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies.
Related Articles Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies.
Nat Biotechnol. 2005 Jun;23(6):736-40
Authors: Züger S, Iwai H
Segmental isotopic labeling of proteins using protein ligation is a recently established in vitro method for incorporating isotopes into one domain or region of a protein to reduce the complexity of NMR spectra, thereby facilitating...
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[ASAP] Structural, Dynamic, and Functional Characterization of a DnaX Mini-intein Derived from Spirulina platensis Provides Important Insights into Intein-Mediated Catalysis of Protein Splicing
Linear Mode
