[ASAP] Solving the Conundrum: Widespread Proteins Annotated for Urea Metabolism in Bacteria Are Carboxyguanidine Deiminases Mediating Nitrogen Assimilation from Guanidine
[ASAP] Solving the Conundrum: Widespread Proteins Annotated for Urea Metabolism in Bacteria Are Carboxyguanidine Deiminases Mediating Nitrogen Assimilation from Guanidine
[ASAP] Ramping Recombinant Protein Expression in Bacteria
Ramping Recombinant Protein Expression in Bacteria
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.0c00411/20200603/images/medium/bi0c00411_0002.gif
Biochemistry
DOI: 10.1021/acs.biochem.0c00411
http://feeds.feedburner.com/~r/acs/bichaw/~4/U2VaWVAIeI8
More...
nmrlearner
Journal club
0
06-05-2020 08:56 AM
[ASAP] Urea-Assisted Reconstitution of Discoidal High-Density Lipoprotein
Urea-Assisted Reconstitution of Discoidal High-Density Lipoprotein
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.0c00075/20200409/images/medium/bi0c00075_0007.gif
Biochemistry
DOI: 10.1021/acs.biochem.0c00075
http://feeds.feedburner.com/~r/acs/bichaw/~4/1vAmh7yGQXU
More...
nmrlearner
Journal club
0
04-20-2020 05:10 PM
[ASAP] Coupling Green Fluorescent Protein Expression with Chemical Modification to Probe Functionally Relevant Riboswitch Conformations in Live Bacteria
Coupling Green Fluorescent Protein Expression with Chemical Modification to Probe Functionally Relevant Riboswitch Conformations in Live Bacteria
https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.8b00316/20180626/images/medium/bi-2018-003163_0005.gif
Biochemistry
DOI: 10.1021/acs.biochem.8b00316
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/Etm4Uq6nj6M
More...
nmrlearner
Journal club
0
06-27-2018 01:51 AM
[Question from NMRWiki Q&A forum] What are the biggest differences in solving NMR structures of DNA or RNA vs. proteins?
What are the biggest differences in solving NMR structures of DNA or RNA vs. proteins?
I've only solved NMR structure of globular proteins (actually in a complex with a short stretch of DNA), but never a structure of nucleic acid alone.
What are (are there?) the major differences in the approaches when working on a structure of nucleic acid - in terms of choice of the NMR technique, maybe specific parameters and structure simulation?
Are distance restraints alone sufficient to obtain structure of DNA or RNA?
Thank you in advance!
nmrlearner
News from other NMR forums
0
06-28-2016 04:38 AM
[NMR paper] Investigation of proteins in living bacteria with in-cell NMR experiments.
Investigation of proteins in living bacteria with in-cell NMR experiments.
Related Articles Investigation of proteins in living bacteria with in-cell NMR experiments.
Top Curr Chem. 2008;273:203-14
Authors: Dötsch V
Abstract
In recent years NMR methods have been developed that enable the observation of proteins insideliving bacterial cells. Because of the sensitivity of the chemical shift to environmental changesthese in-cell NMR experiments can be used to study protein conformation, molecular interaction ordynamics in a*protein's natural...
nmrlearner
Journal club
0
04-24-2013 09:48 PM
The relative and regional stabilities of the hamster, mouse, rabbit and bovine prion proteins towards urea unfolding assessed by NMR and CD spectroscopies.
The relative and regional stabilities of the hamster, mouse, rabbit and bovine prion proteins towards urea unfolding assessed by NMR and CD spectroscopies.
The relative and regional stabilities of the hamster, mouse, rabbit and bovine prion proteins towards urea unfolding assessed by NMR and CD spectroscopies.
Biochemistry. 2011 Jul 29;
Authors: Julien O, Chatterjee S, Bjorndahl TC, Sweeting B, Acharya S, Semenchenko V, Chakrabartty A, Pai EF, Wishart DS, Sykes BD, Cashman NR
The residue specific urea-induced unfolding patterns of recombinant...
nmrlearner
Journal club
0
08-02-2011 11:40 AM
[NMR paper] Nitrogen-15 NMR studies of nitrogen metabolism in Picea glauca buds.
Nitrogen-15 NMR studies of nitrogen metabolism in Picea glauca buds.
Related Articles Nitrogen-15 NMR studies of nitrogen metabolism in Picea glauca buds.
Plant Physiol Biochem. 2004 Dec;42(10):803-9
Authors: Bagh K, Hiraoki T, Thorpe TA, Vogel HJ
In vivo (15)N nuclear magnetic resonance (NMR) as well as (15)N solid-state magic angle spinning (MAS) NMR spectroscopy were used to investigate nitrogen metabolism in cultured white spruce (Picea glauca) buds. Long-term as well as short-term experiments were carried out involving the use of...
nmrlearner
Journal club
0
11-24-2010 10:03 PM
[Question from NMRWiki Q&A forum] Best pulse sequence for HSQC in Guanidine-HCl
Best pulse sequence for HSQC in Guanidine-HCl
Hello,
I would like to acquire a series of HSQC spectra of an 15N-labeled 12 kDa protein unfolding (over the course of hours) in 3M GuaHCl. I have access to a Varian 600 spectrometer with a conventional probe. Due to the high ionic strength of the sample, tuning and shimming is a little difficult. However, I can record a sort-of-decent HSQC using a WaterGate gradient enhanced WGgNhsqc sequence. I am limited to using a protein concentration of about 250 uM. I've determined the pw90 to be 12.125 at a tpwr of 63.
Using WGgNhsqc, the protein...
![[ASAP] Solving the Conundrum: Widespread Proteins Annotated for Urea Metabolism in Bacteria Are Carboxyguanidine Deiminases Mediating Nitrogen Assimilation from Guanidine](http://www.bionmr.com/forum/iconimages/journal-club-9/%5Basap%5D-solving-conundrum-widespread-proteins-annotated-urea-metabolism-bacteria-carboxyguanidine-deiminases-mediating-nitrogen-assimilation-guanidine_ltr.gif)
[ASAP] Solving the Conundrum: Widespread Proteins Annotated for Urea Metabolism in Bacteria Are Carboxyguanidine Deiminases Mediating Nitrogen Assimilation from Guanidine
Linear Mode
