[ASAP] In Situ, Protein-Mediated Generation of a Photochemically Active Chlorophyll Analogue in a Mutant Bacterial Photosynthetic Reaction Center

		BioNMR > Educational resources > Journal club
[ASAP] In Situ, Protein-Mediated Generation of a Photochemically Active Chlorophyll Analogue in a Mutant Bacterial Photosynthetic Reaction Center

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

04-10-2021, 03:40 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

[ASAP] In Situ, Protein-Mediated Generation of a Photochemically Active Chlorophyll Analogue in a Mutant Bacterial Photosynthetic Reaction Center

[ASAP] In Situ, Protein-Mediated Generation of a Photochemically Active Chlorophyll Analogue in a Mutant Bacterial Photosynthetic Reaction Center

Biochemistry
DOI: 10.1021/acs.biochem.1c00137

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[ASAP] Unraveling the Reaction Mechanisms of SiO Anodes for Li-Ion Batteries by Combining in Situ 7Li and ex Situ 7Li/29Si Solid-State NMR Spectroscopy Unraveling the Reaction Mechanisms of SiO Anodes for Li-Ion Batteries by Combining in Situ 7Li and ex Situ 7Li/29Si Solid-State NMR Spectroscopy Keitaro Kitada, Oliver Pecher, Pieter C. M. M. Magusin, Matthias F. Groh, Robert S. Weatherup, Clare P. Grey https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.9b01589/20190423/images/medium/ja-2019-01589u_0009.gif Journal of the American Chemical Society DOI: 10.1021/jacs.9b01589 http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA...	nmrlearner	Journal club	0	04-23-2019 07:54 PM
Assignment of NMR resonances of protons covalently bound to photochemically active cofactors in photosynthetic reaction centers by 13C–1H photo-CIDNP MAS-J-HMQC experiment From The DNP-NMR Blog: Assignment of NMR resonances of protons covalently bound to photochemically active cofactors in photosynthetic reaction centers by 13C–1H photo-CIDNP MAS-J-HMQC experiment Bielytskyi, Pavlo, Daniel Gräsing, Stefan Zahn, Kaustubh R. Mote, A. Alia, P.K. Madhu, and Jörg Matysik. “Assignment of NMR Resonances of Protons Covalently Bound to Photochemically Active Cofactors in Photosynthetic Reaction Centers by 13C–1H Photo-CIDNP MAS-J-HMQC Experiment.” Journal of Magnetic Resonance 298 (January 2019): 64–76. https://doi.org/10.1016/j.jmr.2018.11.013.	nmrlearner	News from NMR blogs	0	03-24-2019 10:41 PM
[NMR paper] Assignment of NMR resonances of protons covalently bound to photochemically active cofactors in photosynthetic reaction centers by 13C–1H photo-CIDNP MAS-J-HMQC experiment Assignment of NMR resonances of protons covalently bound to photochemically active cofactors in photosynthetic reaction centers by 13C–1H photo-CIDNP MAS-J-HMQC experiment Publication date: January 2019 Source: Journal of Magnetic Resonance, Volume 298 Author(s): Pavlo Bielytskyi, Daniel Gräsing, Stefan Zahn, Kaustubh R. Mote, A. Alia, P.K. Madhu, Jörg Matysik Abstract	nmrlearner	Journal club	0	01-07-2019 05:49 AM
[NMR paper] 13C->1H transfer of light-induced hyperpolarization allows for selective detection of protons in frozen photosynthetic reaction center 13C->1H transfer of light-induced hyperpolarization allows for selective detection of protons in frozen photosynthetic reaction center Publication date: Available online 8 June 2018 Source:Journal of Magnetic Resonance</br> Author(s): Pavlo Bielytskyi, Daniel Gräsing, Kaustubh R. Mote, Karthick Babu Sai Sankar Gupta, Shimon Vega, P.K. Madhu, A. Alia, Jörg Matysik</br> In the present study, we exploit the light-induced hyperpolarization occurring on 13C nuclei due to the solid-state photochemically induced dynamic nuclear polarization (photo-CIDNP)...	nmrlearner	Journal club	0	06-08-2018 06:35 PM
[NMR paper] Analysis of the electronic structure of the Special Pair of a Bacterial Photosynthetic Reaction Center by (13) C Photochemically Induced Dynamic Nuclear Polarization Magic-Angle Spinning NMR Using a Double-Quantum Axis. Analysis of the electronic structure of the Special Pair of a Bacterial Photosynthetic Reaction Center by (13) C Photochemically Induced Dynamic Nuclear Polarization Magic-Angle Spinning NMR Using a Double-Quantum Axis. Related Articles Analysis of the electronic structure of the Special Pair of a Bacterial Photosynthetic Reaction Center by (13) C Photochemically Induced Dynamic Nuclear Polarization Magic-Angle Spinning NMR Using a Double-Quantum Axis. Photochem Photobiol. 2017 Jul 26;: Authors: Najdanova M, Gräsing D, Alia A, Matysik J ...	nmrlearner	Journal club	0	07-27-2017 04:37 PM
Photochemically Induced Dynamic Nuclear Polarization Observed by Solid-State NMR in a Uniformly (13)C-Isotope-Labeled Photosynthetic Reaction Center From The DNP-NMR Blog: Photochemically Induced Dynamic Nuclear Polarization Observed by Solid-State NMR in a Uniformly (13)C-Isotope-Labeled Photosynthetic Reaction Center Paul, S., et al., Photochemically Induced Dynamic Nuclear Polarization Observed by Solid-State NMR in a Uniformly (13)C-Isotope-Labeled Photosynthetic Reaction Center. J Phys Chem B, 2015. 119(43): p. 13897-903. http://www.ncbi.nlm.nih.gov/pubmed/26110356	nmrlearner	News from NMR blogs	0	03-30-2016 08:39 PM
Photochemically Induced Dynamic Nuclear Polarization Observed by Solid-State NMR in a Uniformly C-Isotope-Labeled Photosynthetic Reaction Center From The DNP-NMR Blog: Photochemically Induced Dynamic Nuclear Polarization Observed by Solid-State NMR in a Uniformly C-Isotope-Labeled Photosynthetic Reaction Center Paul, S., et al., Photochemically Induced Dynamic Nuclear Polarization Observed by Solid-State NMR in a Uniformly C-Isotope-Labeled Photosynthetic Reaction Center. J Phys Chem B, 2015. http://www.ncbi.nlm.nih.gov/pubmed/26110356	nmrlearner	News from NMR blogs	0	07-20-2015 09:54 PM
[NMR paper] Photochemically Induced Dynamic Nuclear Polarization Observed by Solid-state NMR in a Uniformly (13)C-isotope Labeled Photosynthetic Reaction Center. Photochemically Induced Dynamic Nuclear Polarization Observed by Solid-state NMR in a Uniformly (13)C-isotope Labeled Photosynthetic Reaction Center. Related Articles Photochemically Induced Dynamic Nuclear Polarization Observed by Solid-state NMR in a Uniformly (13)C-isotope Labeled Photosynthetic Reaction Center. J Phys Chem B. 2015 Jun 25; Authors: Paul S, Bode BE, Matysik J, Alia A Abstract A sample of solubilized and quinone-depleted reaction centers (RC) from the purple bacterium Rhodobacter (R.) sphaeroides wild-type (WT)...	nmrlearner	Journal club	0	06-26-2015 09:55 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off