[ASAP] Significance of the Protein Interface Configuration for Allostery in Imidazole Glycerol Phosphate Synthase

		BioNMR > Educational resources > Journal club
[ASAP] Significance of the Protein Interface Configuration for Allostery in Imidazole Glycerol Phosphate Synthase

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

07-16-2020, 09:01 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

[ASAP] Significance of the Protein Interface Configuration for Allostery in Imidazole Glycerol Phosphate Synthase

[ASAP] Significance of the Protein Interface Configuration for Allostery in Imidazole Glycerol Phosphate Synthase

Biochemistry
DOI: 10.1021/acs.biochem.0c00332

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Allosteric Communication Disrupted by a Small MoleculeBinding to the Imidazole Glycerol Phosphate Synthase Protein–ProteinInterface Allosteric Communication Disrupted by a Small MoleculeBinding to the Imidazole Glycerol Phosphate Synthase Protein–ProteinInterface http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.6b00859/20161111/images/medium/bi-2016-00859x_0008.gif Biochemistry DOI: 10.1021/acs.biochem.6b00859 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/SQoUyC3SKLE More...	nmrlearner	Journal club	0	11-19-2016 08:35 PM
Effects of solvent concentration and composition on protein dynamics: 13C MAS NMR studies of elastin in glycerol-water mixtures Effects of solvent concentration and composition on protein dynamics: 13C MAS NMR studies of elastin in glycerol-water mixtures Publication date: Available online 25 April 2015 Source:Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics</br> Author(s): Dominik Demuth , Nils Haase , Daniel Malzacher , Michael Vogel</br> We use 13C CP MAS NMR to investigate the dependence of elastin dynamics on the concentration and composition of the solvent at various temperatures. For elastin in pure glycerol, line-shape analysis shows that larger-scale fluctuations of...	nmrlearner	Journal club	0	04-26-2015 03:28 AM
Protonation States of the Tryptophan Synthase Internal Aldimine Active Site from Solid-State NMR Spectroscopy: Direct Observation of the Protonated Schiff Base Linkage to Pyridoxal-5?-Phosphate Protonation States of the Tryptophan Synthase Internal Aldimine Active Site from Solid-State NMR Spectroscopy: Direct Observation of the Protonated Schiff Base Linkage to Pyridoxal-5?-Phosphate Bethany G. Caulkins, Baback Bastin, Chen Yang, Thomas J. Neubauer, Robert P. Young, Eduardo Hilario, Yu-ming M. Huang, Chia-en A. Chang, Li Fan, Michael F. Dunn, Michael J. Marsella and Leonard J. Mueller http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja506267d/aop/images/medium/ja-2014-06267d_0003.gif Journal of the American Chemical Society...	nmrlearner	Journal club	0	09-04-2014 02:25 AM
[NMR paper] Crystallographic and NMR evidence for flexibility in oligosaccharyltransferases and its catalytic significance. Crystallographic and NMR evidence for flexibility in oligosaccharyltransferases and its catalytic significance. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-cellhub.gif Related Articles Crystallographic and NMR evidence for flexibility in oligosaccharyltransferases and its catalytic significance. Structure. 2013 Jan 8;21(1):32-41 Authors: Nyirenda J, Matsumoto S, Saitoh T, Maita N, Noda NN, Inagaki F, Kohda D Abstract Oligosaccharyltransferase (OST) is a membrane-bound...	nmrlearner	Journal club	0	07-03-2013 01:46 PM
NMR insights into protein allostery NMR insights into protein allostery 15 March 2012 Publication year: 2012 Source:Archives of Biochemistry and Biophysics, Volume 519, Issue 2</br> </br> Allosterism is one of nature’s principal methods for regulating protein function. Allosterism utilizes ligand binding at one site to regulate the function of the protein by modulating the structure and dynamics of a distant binding site. In this review, we first survey solution NMR techniques and how they may be applied to the study of allostery. Subsequently, we describe several examples of application of NMR to protein...	nmrlearner	Journal club	0	02-03-2013 10:13 AM
Glycerol and Glycerol Carbonate as ultraviscous solvents for mixture analysis by NMR Glycerol and Glycerol Carbonate as ultraviscous solvents for mixture analysis by NMR Publication year: 2011 Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 1 July 2011</br> Pedro, Lameiras , Leslie, Boudesocque , Zéphirin, Mouloungui , Jean-Hugues, Renault , Jean-Michel, Wieruszeski , ...</br> NMR of weakly polar analytes in an apolar ultraviscous solvent has recently been proposed for mixture analysis as a pertinent alternative to the DOSY experiment. The present article reports the first use of glycerol and glycerol carbonate as polar...	nmrlearner	Journal club	0	07-03-2011 05:40 AM
[NMR paper] Phosphate ions in bone: identification of a calcium-organic phosphate complex by 31P Phosphate ions in bone: identification of a calcium-organic phosphate complex by 31P solid-state NMR spectroscopy at early stages of mineralization. Related Articles Phosphate ions in bone: identification of a calcium-organic phosphate complex by 31P solid-state NMR spectroscopy at early stages of mineralization. Calcif Tissue Int. 2003 May;72(5):610-26 Authors: Wu Y, Ackerman JL, Strawich ES, Rey C, Kim HM, Glimcher MJ Previous 31P cross-polarization and differential cross-polarization magic angle spinning (CP/MAS and DCP/MAS) solid-state NMR...	nmrlearner	Journal club	0	11-24-2010 09:01 PM
[NMR paper] Molecular conformation of porcine amelogenins and its significance in protein-mineral Molecular conformation of porcine amelogenins and its significance in protein-mineral interaction: 1H-NMR photo-CIDNP study. Related Articles Molecular conformation of porcine amelogenins and its significance in protein-mineral interaction: 1H-NMR photo-CIDNP study. J Biol Buccale. 1990 Sep;18(3):189-94 Authors: Aoba T, Kawano K, Moreno EC The present 1H-nmr study was undertaken to investigate the molecular structure of porcine amelogenins in solution using photo-CIDNP (chemically induced dynamic nuclear polarization). The proteins of interest...	nmrlearner	Journal club	0	08-21-2010 11:04 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off