[ASAP] Magnetically Aligned Lipid Bilayers with High Cholesterol for Solid-State NMR of Membrane Proteins

		BioNMR > Educational resources > Journal club
[ASAP] Magnetically Aligned Lipid Bilayers with High Cholesterol for Solid-State NMR of Membrane Proteins

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

07-19-2022, 05:10 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

[ASAP] Magnetically Aligned Lipid Bilayers with High Cholesterol for Solid-State NMR of Membrane Proteins

[ASAP] Magnetically Aligned Lipid Bilayers with High Cholesterol for Solid-State NMR of Membrane Proteins

Biochemistry
DOI: 10.1021/acs.biochem.2c00262

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Membrane proteins in magnetically aligned phospholipid polymer discs for solid-state NMR spectroscopy. Membrane proteins in magnetically aligned phospholipid polymer discs for solid-state NMR spectroscopy. http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Membrane proteins in magnetically aligned phospholipid polymer discs for solid-state NMR spectroscopy. Biochim Biophys Acta Biomembr. 2020 May 01;:183333 Authors: Park SH, Wu J, Yao Y, Singh C, Tian Y, Marassi FM, Opella SJ Abstract Well-hydrated phospholipid bilayers provide a near-native...	nmrlearner	Journal club	0	05-07-2020 09:07 PM
Rotational Diffusion of Membrane Proteins Probed by Anisotropic T2 and T1? NMR Relaxation in Aligned Lipid Bilayers Rotational Diffusion of Membrane Proteins Probed by Anisotropic T2 and T1? NMR Relaxation in Aligned Lipid Bilayers Publication date: 2 February 2018 Source:Biophysical Journal, Volume 114, Issue 3, Supplement 1</br> Author(s): Alexander Nevzorov, Emmanuel Awosanya</br> </br></br> </br></br> More...	nmrlearner	Journal club	0	02-07-2018 03:41 PM
[NMR paper] Solid-State NMR of Highly 13C-Enriched Cholesterol in Lipid Bilayers. Solid-State NMR of Highly 13C-Enriched Cholesterol in Lipid Bilayers. Related Articles Solid-State NMR of Highly 13C-Enriched Cholesterol in Lipid Bilayers. Methods. 2018 Jan 20;: Authors: Della Ripa LA, Petros ZA, Cioffi AG, Piehl DW, Courtney JM, Burke MD, Rienstra CM Abstract Cholesterol (Chol) is vital for cell function as it is essential to a myriad of biochemical and biophysical processes. The atomistic details of Chol's interactions with phospholipids and proteins is therefore of fundamental interest, and NMR offers unique...	nmrlearner	Journal club	0	01-26-2018 12:08 PM
[NMR paper] Optimization of cross-polarization at low radiofrequency fields for sensitivity enhancement in solid-state NMR of membrane proteins reconstituted in magnetically aligned bicelles. Optimization of cross-polarization at low radiofrequency fields for sensitivity enhancement in solid-state NMR of membrane proteins reconstituted in magnetically aligned bicelles. Optimization of cross-polarization at low radiofrequency fields for sensitivity enhancement in solid-state NMR of membrane proteins reconstituted in magnetically aligned bicelles. J Magn Reson. 2015 Apr 28;256:14-22 Authors: Koroloff SN, Nevzorov AA Abstract Solid-state NMR (ssNMR) of oriented membrane proteins (MPs) is capable of providing...	nmrlearner	Journal club	0	05-13-2015 02:01 PM
[NMR paper] Optimization of cross-polarization at low radiofrequency fields for sensitivity enhancement in solid-state NMR of membrane proteins reconstituted in magnetically aligned bicelles Optimization of cross-polarization at low radiofrequency fields for sensitivity enhancement in solid-state NMR of membrane proteins reconstituted in magnetically aligned bicelles Publication date: Available online 28 April 2015 Source:Journal of Magnetic Resonance</br> Author(s): Sophie N. Koroloff , Alexander A. Nevzorov</br> Solid-state NMR (ssNMR) of oriented membrane proteins (MPs) is capable of providing structural and dynamic information at nearly physiological conditions. However, NMR experiments performed on oriented membrane proteins generally suffer from...	nmrlearner	Journal club	0	04-28-2015 12:40 PM
[NMR paper] Dipolar Assisted Assignment Protocol (DAAP) for MAS solid-state NMR of rotationally aligned membrane proteins in phospholipid bilayers. Dipolar Assisted Assignment Protocol (DAAP) for MAS solid-state NMR of rotationally aligned membrane proteins in phospholipid bilayers. Dipolar Assisted Assignment Protocol (DAAP) for MAS solid-state NMR of rotationally aligned membrane proteins in phospholipid bilayers. J Magn Reson. 2014 Mar 1;242C:224-232 Authors: Das BB, Zhang H, Opella SJ Abstract A method for making resonance assignments in magic angle spinning solid-state NMR spectra of membrane proteins that utilizes the range of heteronuclear dipolar coupling...	nmrlearner	Journal club	0	04-06-2014 02:01 AM
Dipolar Assisted Assignment Protocol (DAAP) for MAS solid-state NMR of Rotationally Aligned Membrane Proteins in Phospholipid Bilayers Dipolar Assisted Assignment Protocol (DAAP) for MAS solid-state NMR of Rotationally Aligned Membrane Proteins in Phospholipid Bilayers Publication date: Available online 1 March 2014 Source:Journal of Magnetic Resonance</br> Author(s): Bibhuti B. Das , Hua Zhang , Stanley J. Opella</br> A method for making resonance assignments in magic angle spinning solid-state NMR spectra of membrane proteins that utilizes the range of hetero-nuclear dipolar coupling frequencies in combination with conventional chemical shift based assignment methods is demonstrated. The...	nmrlearner	Journal club	0	03-01-2014 11:11 AM
Multidimensional oriented solid-state NMR experiments enable the sequential assignment of uniformly 15N labeled integral membrane proteins in magnetically aligned lipid bilayers Multidimensional oriented solid-state NMR experiments enable the sequential assignment of uniformly 15N labeled integral membrane proteins in magnetically aligned lipid bilayers Abstract Oriented solid-state NMR is the most direct methodology to obtain the orientation of membrane proteins with respect to the lipid bilayer. The method consists of measuring 1H-15N dipolar couplings (DC) and 15N anisotropic chemical shifts (CSA) for membrane proteins that are uniformly aligned with respect to the membrane bilayer. A significant advantage of this approach is that tilt and azimuthal...	nmrlearner	Journal club	0	10-10-2011 06:27 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off