[ASAP] Irreversible Inactivation of SARS-CoV-2 by Lectin Engagement with Two Glycan Clusters on the Spike Protein

		BioNMR > Educational resources > Journal club
[ASAP] Irreversible Inactivation of SARS-CoV-2 by Lectin Engagement with Two Glycan Clusters on the Spike Protein

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

06-21-2023, 04:00 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,574

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

[ASAP] Irreversible Inactivation of SARS-CoV-2 by Lectin Engagement with Two Glycan Clusters on the Spike Protein

[ASAP] Irreversible Inactivation of SARS-CoV-2 by Lectin Engagement with Two Glycan Clusters on the Spike Protein

Biochemistry
DOI: 10.1021/acs.biochem.3c00109

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Antcin A, a phytosterol regulates SARS-CoV-2 spike protein ... - Wiley Antcin A, a phytosterol regulates SARS-CoV-2 spike protein ... - Wiley Antcin A, a phytosterol regulates SARS-CoV-2 spike protein ... Wiley Read here	nmrlearner	Online News	0	04-12-2023 09:19 AM
[ASAP] Energetics of Spike Protein Opening of SARS-CoV-1 and SARS-CoV-2 and Its Variants of Concern: Implications in Host Receptor Scanning and Transmission Energetics of Spike Protein Opening of SARS-CoV-1 and SARS-CoV-2 and Its Variants of Concern: Implications in Host Receptor Scanning and Transmission https://pubs.acs.org/cms/10.1021/acs.biochem.2c00301/asset/images/medium/bi2c00301_0006.gif Biochemistry DOI: 10.1021/acs.biochem.2c00301 More...	nmrlearner	Journal club	0	10-06-2022 06:14 PM
[ASAP] NMR Experiments Provide Insights into Ligand-Binding to the SARS-CoV-2 Spike Protein Receptor-Binding Domain NMR Experiments Provide Insights into Ligand-Binding to the SARS-CoV-2 Spike Protein Receptor-Binding Domain Robert Creutznacher, Thorben Maass, Barbora Veselkova, George Ssebyatika, Thomas Krey, Martin Empting, Norbert Tautz, Martin Frank, Knut Ko?lbel, Charlotte Uetrecht, and Thomas Peters https://pubs.acs.org/cms/10.1021/jacs.2c05603/asset/images/medium/ja2c05603_0004.gif Journal of the American Chemical Society DOI: 10.1021/jacs.2c05603	nmrlearner	Journal club	0	07-16-2022 01:22 AM
[ASAP] Identifying Distinct Structural Features of the SARS-CoV-2 Spike Protein Fusion Domain Essential for Membrane Interaction Identifying Distinct Structural Features of the SARS-CoV-2 Spike Protein Fusion Domain Essential for Membrane Interaction https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.1c00543/20210927/images/medium/bi1c00543_0006.gif Biochemistry DOI: 10.1021/acs.biochem.1c00543 http://feeds.feedburner.com/~r/acs/bichaw/~4/NkCMDqCUOyo More...	nmrlearner	Journal club	0	09-29-2021 12:23 PM
[ASAP] Comparative Perturbation-Based Modeling of the SARS-CoV-2 Spike Protein Binding with Host Receptor and Neutralizing Antibodies: Structurally Adaptable Allosteric Communication Hotspots Define Spike Sites Targeted by Global Circulating Mutation Comparative Perturbation-Based Modeling of the SARS-CoV-2 Spike Protein Binding with Host Receptor and Neutralizing Antibodies: Structurally Adaptable Allosteric Communication Hotspots Define Spike Sites Targeted by Global Circulating Mutations https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.1c00139/20210426/images/medium/bi1c00139_0013.gif Biochemistry DOI: 10.1021/acs.biochem.1c00139 http://feeds.feedburner.com/~r/acs/bichaw/~4/2WX5bA-38FE More...	nmrlearner	Journal club	0	04-27-2021 10:40 AM
[NMR paper] Automated glycan assembly of 19F labelled glycan probes enables high-throughput NMR studies of protein-glycan interactions Automated glycan assembly of 19F labelled glycan probes enables high-throughput NMR studies of protein-glycan interactions Protein-glycan interactions mediate important biological processes, including pathogen host invasion and cellular communication. Major challenges to monitoring these low affinity interactions are the required high sensitivity of a biophysical assay and to cover a breath of synthetic well-defined structures. Here, we showcase an expedite approach that integrates automated glycan assembly (AGA) of 19 F labelled probes and high-throughput NMR methods, enabling the study...	nmrlearner	Journal club	0	03-30-2021 11:12 PM
Structural investigation of glycan recognition by the ERAD quality control lectin Yos9 Structural investigation of glycan recognition by the ERAD quality control lectin Yos9 Abstract Yos9 is an essential component of the endoplasmic reticulum associated protein degradation (ERAD) system that is responsible for removing terminally misfolded proteins from the ER lumen and mediating proteasomal degradation in the cytosol. Glycoproteins that fail to attain their native conformation in the ER expose a distinct oligosaccharide structure, a terminal Î±1,6-linked mannose residue, that is specifically recognized by the mannose 6-phoshate...	nmrlearner	Journal club	0	11-25-2018 06:02 AM
[NMR paper] Fluorinated Carbohydrates as Lectin Ligands: Dissecting Glycan-Cyanovirin Interactions by Using 19 F NMR Spectroscopy. Fluorinated Carbohydrates as Lectin Ligands: Dissecting Glycan-Cyanovirin Interactions by Using 19 F NMR Spectroscopy. Fluorinated Carbohydrates as Lectin Ligands: Dissecting Glycan-Cyanovirin Interactions by Using 19 F NMR Spectroscopy. Chemistry. 2013 Feb 28; Authors: Matei E, André S, Glinschert A, Infantino AS, Oscarson S, Gabius HJ, Gronenborn AM Abstract NMR spectroscopy and isothermal titration calorimetry (ITC) are powerful methods to investigate ligand-protein interactions. Here, we present a versatile and sensitive fluorine NMR...	nmrlearner	Journal club	0	03-01-2013 09:57 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off