[ASAP] Innate Conformational Dynamics Drive Binding Specificity in Anti-Apoptotic Proteins Mcl-1 and Bcl-2

		BioNMR > Educational resources > Journal club
[ASAP] Innate Conformational Dynamics Drive Binding Specificity in Anti-Apoptotic Proteins Mcl-1 and Bcl-2

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

05-17-2023, 05:43 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

[ASAP] Innate Conformational Dynamics Drive Binding Specificity in Anti-Apoptotic Proteins Mcl-1 and Bcl-2

[ASAP] Innate Conformational Dynamics Drive Binding Specificity in Anti-Apoptotic Proteins Mcl-1 and Bcl-2

Biochemistry
DOI: 10.1021/acs.biochem.2c00709

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[ASAP] Aromatic Interactions Drive the Coupled Folding and Binding of the Intrinsically Disordered Sesbania mosaic Virus VPg Protein Aromatic Interactions Drive the Coupled Folding and Binding of the Intrinsically Disordered Sesbania mosaic Virus VPg Protein https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.0c00721/20201203/images/medium/bi0c00721_0009.gif Biochemistry DOI: 10.1021/acs.biochem.0c00721 http://feeds.feedburner.com/~r/acs/bichaw/~4/MoSUods3vk8 More...	nmrlearner	Journal club	0	12-04-2020 03:46 PM
[ASAP] Conformational Dynamics in the Binding-Protein-Independent Mutant of the Escherichia coli Maltose Transporter, MalG511, and Its Interaction with Maltose Binding Protein Conformational Dynamics in the Binding-Protein-Independent Mutant of the Escherichia coli Maltose Transporter, MalG511, and Its Interaction with Maltose Binding Protein https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.8b00266/20180511/images/medium/bi-2018-00266j_0010.gif Biochemistry DOI: 10.1021/acs.biochem.8b00266 http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/bichaw/~4/dvY2l8iYVK4 More...	nmrlearner	Journal club	0	05-12-2018 02:10 AM
[NMR paper] High-Quality NMR Structure of Human Anti-Apoptotic Protein Domain Mcl-1(171-327) for Cancer Drug Design. High-Quality NMR Structure of Human Anti-Apoptotic Protein Domain Mcl-1(171-327) for Cancer Drug Design. Related Articles High-Quality NMR Structure of Human Anti-Apoptotic Protein Domain Mcl-1(171-327) for Cancer Drug Design. PLoS One. 2014;9(5):e96521 Authors: Liu G, Poppe L, Aoki K, Yamane H, Lewis J, Szyperski T Abstract A high-quality NMR solution structure is presented for protein hMcl-1(171-327) which comprises residues 171-327 of the human anti-apoptotic protein Mcl-1 (hMcl-1). Since this construct contains the three...	nmrlearner	Journal club	0	05-03-2014 10:42 PM
[NMR paper] Targeting of p53 Peptide Analogues to Anti-Apoptotic Bcl-2 Family Proteins as Revealed by NMR Spectroscopy. Targeting of p53 Peptide Analogues to Anti-Apoptotic Bcl-2 Family Proteins as Revealed by NMR Spectroscopy. Related Articles Targeting of p53 Peptide Analogues to Anti-Apoptotic Bcl-2 Family Proteins as Revealed by NMR Spectroscopy. Biochem Biophys Res Commun. 2013 Dec 13; Authors: Shin JS, Ha JH, Chi SW Abstract Inhibition of the interaction between the p53 tumor suppressor and its negative regulator MDM2 is of great importance to cancer therapy. The anti-apoptotic Bcl-2 family proteins are also attractive anti-cancer molecular...	nmrlearner	Journal club	0	12-18-2013 04:00 PM
Targeting of p53 Peptide Analogues to Anti-Apoptotic Bcl-2 Family Proteins as Revealed by NMR Spectroscopy Targeting of p53 Peptide Analogues to Anti-Apoptotic Bcl-2 Family Proteins as Revealed by NMR Spectroscopy Publication date: Available online 14 December 2013 Source:Biochemical and Biophysical Research Communications</br> Author(s): Jae-Sun Shin , Ji-Hyang Ha , Seung-Wook Chi</br> Inhibition of the interaction between the p53 tumor suppressor and its negative regulator MDM2 is of great importance to cancer therapy. The anti-apoptotic Bcl-2 family proteins are also attractive anti-cancer molecular targets, as they are key regulators of apoptotic cell death....	nmrlearner	Journal club	0	12-14-2013 04:48 PM
[NMR paper] Unique Structure and Dynamics of the EphA5 Ligand Binding Domain Mediate Its Binding Specificity as Revealed by X-ray Crystallography, NMR and MD Simulations. Unique Structure and Dynamics of the EphA5 Ligand Binding Domain Mediate Its Binding Specificity as Revealed by X-ray Crystallography, NMR and MD Simulations. Unique Structure and Dynamics of the EphA5 Ligand Binding Domain Mediate Its Binding Specificity as Revealed by X-ray Crystallography, NMR and MD Simulations. PLoS One. 2013;8(9):e74040 Authors: Huan X, Shi J, Lim L, Mitra S, Zhu W, Qin H, Pasquale EB, Song J Abstract The 16 EphA and EphB receptors represent the largest family of receptor tyrosine kinases, and their interactions...	nmrlearner	Journal club	0	10-03-2013 03:31 PM
Interaction of a putative BH3 domain of clusterin with anti-apoptotic Bcl-2 family proteins as revealed by NMR spectroscopy. Interaction of a putative BH3 domain of clusterin with anti-apoptotic Bcl-2 family proteins as revealed by NMR spectroscopy. Interaction of a putative BH3 domain of clusterin with anti-apoptotic Bcl-2 family proteins as revealed by NMR spectroscopy. Biochem Biophys Res Commun. 2011 Apr 19; Authors: Lee DH, Ha JH, Kim Y, Bae KH, Park JY, Choi WS, Yoon HS, Park SG, Park BC, Yi GS, Chi SW Clusterin (CLU) is a multifunctional glycoprotein that is overexpressed in prostate and breast cancers. Although CLU is known to be involved in the regulation...	nmrlearner	Journal club	0	04-30-2011 12:36 PM
[NMR paper] NMR studies of the anti-apoptotic protein Bcl-xL in micelles. NMR studies of the anti-apoptotic protein Bcl-xL in micelles. Related Articles NMR studies of the anti-apoptotic protein Bcl-xL in micelles. Biochemistry. 2000 Sep 12;39(36):11024-33 Authors: Losonczi JA, Olejniczak ET, Betz SF, Harlan JE, Mack J, Fesik SW The Bcl-2 family of proteins play a pivotal role in the regulation of programmed cell death. One of the postulated mechanisms for the function of these proteins involves the formation of ion channels in membranes. As a first step to structurally characterize these proteins in a membrane...	nmrlearner	Journal club	0	11-19-2010 08:29 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off