[NMR paper] Automated glycan assembly of 19F labelled glycan probes enables high-throughput NMR studies of protein-glycan interactions
Automated glycan assembly of 19F labelled glycan probes enables high-throughput NMR studies of protein-glycan interactions
Protein-glycan interactions mediate important biological processes, including pathogen host invasion and cellular communication. Major challenges to monitoring these low affinity interactions are the required high sensitivity of a biophysical assay and to cover a breath of synthetic well-defined structures. Here, we showcase an expedite approach that integrates automated glycan assembly (AGA) of 19 F labelled probes and high-throughput NMR methods, enabling the study...
nmrlearner
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03-30-2021 11:12 PM
[ASAP] In vitro-Constructed Ribosomes Enable Multi-site Incorporation of Noncanonical Amino Acids into Proteins
In vitro-Constructed Ribosomes Enable Multi-site Incorporation of Noncanonical Amino Acids into Proteins
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.0c00829/20210111/images/medium/bi0c00829_0006.gif
Biochemistry
DOI: 10.1021/acs.biochem.0c00829
http://feeds.feedburner.com/~r/acs/bichaw/~4/ka6wq8GcKfY
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nmrlearner
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01-13-2021 08:38 AM
[NMR paper] Sensitivity enhancement of homonuclear multidimensional NMR correlations for labile sites in proteins, polysaccharides, and nucleic acids.
Sensitivity enhancement of homonuclear multidimensional NMR correlations for labile sites in proteins, polysaccharides, and nucleic acids.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.nature.com-images-lo_npg.gif http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--www.nature.com-images-npg_logo.gif Sensitivity enhancement of homonuclear multidimensional NMR correlations for labile sites in proteins, polysaccharides, and nucleic acids.
Nat Commun. 2020 10 21;11(1):5317
Authors: Novakovic M,...
[ASAP] Improved Method for the Incorporation of Heme Cofactors into Recombinant Proteins Using Escherichia coli Nissle 1917
Improved Method for the Incorporation of Heme Cofactors into Recombinant Proteins Using Escherichia coli Nissle 1917
https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.8b00242/20180425/images/medium/bi-2018-00242b_0009.gif
Biochemistry
DOI: 10.1021/acs.biochem.8b00242
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/xEMFb1XNf30
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nmrlearner
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04-26-2018 02:15 AM
[ASAP] Structural Basis for the Specific Cotranslational Incorporation of p-Boronophenylalanine into Biosynthetic Proteins
Structural Basis for the Specific Cotranslational Incorporation of p-Boronophenylalanine into Biosynthetic Proteins
https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.8b00171/20180424/images/medium/bi-2018-00171m_0003.gif
Biochemistry
DOI: 10.1021/acs.biochem.8b00171
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/VDu0AjuJg94
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nmrlearner
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04-25-2018 01:55 AM
[Question from NMRWiki Q&A forum] OH, NH, SH Labile protons in water nmr
OH, NH, SH Labile protons in water nmr
Hello all,
Just to mirror Craig Grimmer's question about labile protons in non-aqueous solvents, does anyone know of any good articles dealing with labile proton behavior in water?
Thanks,
nmrlearner
News from other NMR forums
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03-11-2015 09:59 PM
[NMR paper] Solution NMR analyses of the C-type carbohydrate recognition domain of DC-SIGNR reveal different binding modes for HIV-derived oligosaccharides and smaller glycan fragments.
Solution NMR analyses of the C-type carbohydrate recognition domain of DC-SIGNR reveal different binding modes for HIV-derived oligosaccharides and smaller glycan fragments.
Related Articles Solution NMR analyses of the C-type carbohydrate recognition domain of DC-SIGNR reveal different binding modes for HIV-derived oligosaccharides and smaller glycan fragments.
J Biol Chem. 2013 Jun 20;
Authors: Probert F, Whittaker SB, Crispin M, Mitchell DA, Dixon AM
Abstract
The C-type lectin DC-SIGNR (Dendritic Cell-Specific ICAM-3-Grabbing...